Anti-CD80 Antibody Picoband™

B7-1/CD80 antibody

Boster Bio Anti-CD80 Antibody Picoband™ catalog # A00196-1. Tested in ELISA, IHC applications. This antibody reacts with Human, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A00196-1
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: ELISA, IHC

Product Name

Anti-CD80 Antibody Picoband™

View all B7-1/CD80 Antibodies

SKU/Catalog Number

A00196-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD80 Antibody Picoband™ catalog # A00196-1. Tested in ELISA, IHC applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD80 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00196-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human CD80 recombinant protein (Position: V35-L244).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00196-1 is reactive to CD80 in Human, Rat

Applications

A00196-1 is guaranteed for ELISA, IHC Boster Guarantee

Observed Molecular Weight

103 kDa

Calculated molecular weight

33.048kDa

Background of B7-1/CD80

Cluster of Differentiation 80 (also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For CD80 (Source: Uniprot.org, NCBI)

Gene Name

CD80

Full Name

T-lymphocyte activation antigen CD80

Weight

33.048kDa

Alternative Names

Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80 CD80 B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7 CD80 molecule T-lymphocyte activation antigen CD80|B-lymphocyte activation antigen B7|CD80 antigen (CD28 antigen ligand 1, B7-1 antigen)|CTLA-4 counter-receptor B7.1|activation B7-1 antigen|costimulatory factor CD80|costimulatory molecule variant IgV-CD80

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD80, check out the CD80 Infographic

CD80 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD80: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00196-1 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Chen X,Gao A,Zhang F,Yang Z,Wang S,Fang Y,Li J,Wang J,Shi W,Wang L, Zheng Y,Sun Y.ILT4 inhibition prevents TAM- and dysfunctional T cell-mediated immunosuppression and enhances the efficacy of anti-PD-L1 therapy in NSCLC with EGFR activation.Theranostics.
Species: Human,Mouse
A00196-1 usage in article: APP:IHC, SAMPLE:HUMAN AND MOUSE LUNG TISSUE, DILUTION:1:500

Du Hy, Dong Lh, Zhao Bj, Fu J, Wang Qq, Chen F, Ou L, Li N, Sun X, Tang Zm, Song Hf. Acta Pharmacol Sin. 2012 Aug;33(8):1047-54. Doi: 10.1038/Aps.2012.54. Epub 2012 Jun 25. Immunostimulatory And Anti-Neoplasm Effects Of A Novel Palindrome Cpg Olig...

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16 Customer Q&As for Anti-CD80 Antibody Picoband™

Question

Is this A00196-1 anti-CD80 antibody reactive to the isotypes of CD80?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

The immunogen of A00196-1 anti-CD80 antibody is E. coli-derived human CD80 recombinant protein (Position: V35-L244). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-31

Question

I was wanting to use to test anti-CD80 antibody A00196-1 on human lymphoid tissue for research purposes, then I may be interested in using anti-CD80 antibody A00196-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

The products we sell, including anti-CD80 antibody A00196-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-31

Question

Will A00196-1 anti-CD80 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-20

Answer

It shows on the product datasheet, A00196-1 anti-CD80 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-20

Question

See attached the WB image, lot number and protocol we used for lymphoid tissue using anti-CD80 antibody A00196-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-05

Question

I was wanting to use your anti-CD80 antibody for ELISA for human lymphoid tissue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lymphoid tissue identification?

Verified Customer

Verified customer

Asked: 2019-08-30

Answer

You can see on the product datasheet, A00196-1 anti-CD80 antibody has been tested for ELISA, IHC on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lymphoid tissue in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-30

Question

Our lab used your anti-CD80 antibody for ELISA on brain in the past. I am using rat, and We want to use the antibody for IHC next. My question regards examining brain as well as gastrocnemius in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

J. Jackson

Verified customer

Asked: 2019-08-30

Answer

I viewed the website and datasheets of our anti-CD80 antibody and it seems that A00196-1 has been tested on rat in both ELISA and IHC. Thus A00196-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-08-30

Question

My question regarding product A00196-1, anti-CD80 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-03

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00196-1 anti-CD80 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-03

Question

We are currently using anti-CD80 antibody A00196-1 for rat tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-02

Answer

The anti-CD80 antibody (A00196-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-02

Question

Is there a BSA free version of anti-CD80 antibody A00196-1 available?

Verified Customer

Verified customer

Asked: 2018-07-12

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD80 antibody A00196-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-07-12

Question

My team were satisfied with the WB result of your anti-CD80 antibody. However we have seen positive staining in brain membrane using this antibody. Is that expected? Could you tell me where is CD80 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-07-21

Answer

According to literature, brain does express CD80. Generally CD80 expresses in membrane. Regarding which tissues have CD80 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Lymphoid tissue, Pubmed ID: 2794510, 1377173

Boster Scientific Support

Answered: 2017-07-21

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymphoid tissue using anti-CD80 antibody A00196-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-07-03

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-07-03

Question

I see that the anti-CD80 antibody A00196-1 works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-06-06

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-06-06

Question

Does anti-CD80 antibody A00196-1 work for ELISA with lymphoid tissue?

E. Carter

Verified customer

Asked: 2016-11-22

Answer

According to the expression profile of lymphoid tissue, CD80 is highly expressed in lymphoid tissue. So, it is likely that anti-CD80 antibody A00196-1 will work for ELISA with lymphoid tissue.

Boster Scientific Support

Answered: 2016-11-22

Question

Is a blocking peptide available for product anti-CD80 antibody (A00196-1)?

J. Roberts

Verified customer

Asked: 2016-03-14

Answer

We do provide the blocking peptide for product anti-CD80 antibody (A00196-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-03-14

Question

Our lab want to know about using your anti-CD80 antibody for dna-templated studies. Has this antibody been tested with western blotting on human tonsil tissue? We would like to see some validation images before ordering.

A. Li

Verified customer

Asked: 2015-04-13

Answer

We appreciate your inquiry. This A00196-1 anti-CD80 antibody is validated on human tonsil tissue, rat spleen tissue. It is guaranteed to work for ELISA, IHC in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2015-04-13

Question

We have been able to see staining in human gastrocnemius. Do you have any suggestions? Is anti-CD80 antibody supposed to stain gastrocnemius positively?

B. Brown

Verified customer

Asked: 2015-03-27

Answer

According to literature gastrocnemius does express CD80. According to Uniprot.org, CD80 is expressed in gastrocnemius, lymphoid tissue, brain, erythroleukemia, among other tissues. Regarding which tissues have CD80 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Lymphoid tissue, Pubmed ID: 2794510, 1377173

Boster Scientific Support

Answered: 2015-03-27

Order DetailsPrice
A00196-1

100μg

$370
A00196-1-10ug

10μg sample (liquid)

$99
A00196-1-Biotin

100 μg Biotin conjugated

$570
A00196-1-Cy3

100 μg Cy3 conjugated

$570
A00196-1-Dylight488

100 μg Dylight488 conjugated

$570
A00196-1-Dylight550

100 μg Dylight550 conjugated

$570
A00196-1-Dylight594

100 μg Dylight594 conjugated

$570
A00196-1-FITC

100 μg FITC conjugated

$570
A00196-1-HRP

100 μg HRP conjugated

$570
A00196-1-APC

100 μg APC conjugated

$670
A00196-1-PE

100 μg PE conjugated

$670
A00196-1-carrier-free

Carrier Free

$370

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