Anti-CDC25C Antibody (Monoclonal, DCS-193)
|Ig Isotype||Mouse IgG1|
|Product Name||Anti-CDC25C Antibody (Monoclonal, DCS-193)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for CDC25C, cell division cycle 25 homolog C (S. pombe) (CDC25C) detection. Tested with WB in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-CDC25C Antibody (Monoclonal, DCS-193) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1018)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Recombinant human Cdc25c.|
Assay Dilutions Overview
Western blot, 1-2μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
Images And Assay Conditions
Boster Kit Box
Protein Target Info (Source: Uniprot.org)
|Protein Name||cell division cycle 25 homolog C(S. pombe)|
|Alternative Names||Protein Cdc25c ;Cdc25c ;|
|Subcellular Localization||CDC25C: Nucleus |Cdc25c: Nucleus.|
|Molecular Weight||50896 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||CDC25C: Functions as a dosage-dependent inducer in mitoticcontrol. Tyrosine protein phosphatase required for progression ofthe cell cycle. When phosphorylated, highly effective inactivating G2 cells into prophase. Directly dephosphorylates CDK1and activates its kinase activity.|
*You can search these to find other products in these research areas.
|Background||Cell division sycle(CDC25) gene product is a protein-tyrosine phosphatase, activates a partially purified p34(cdc2)/cyclin B complex. The cdc25 protein also shares homology with a protein phosphatase with activity against both tyrosine and serine(and thus probably threonine) phosphate residues. CDC25C is expressed predominantly in G2 phase in healer cells. The human gene encodes a protein with a predicted molecular mass of 53,000 daltons whose C-terminal domain shares about 37% sequence identity with the fission yeast cdc25 mitotic inducer. CDC25C gene is mapped to 5q31.|
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Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact email@example.com
Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to firstname.lastname@example.org.
Q: What are some alternative names that could be used to describe this product?A: Some common names include but are not limited to cdc25 antibody, cdc25c antibody