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Product Info Summary
| SKU: | MA1018 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-CDC25C Antibody (Monoclonal, DCS-193)
SKU/Catalog Number
MA1018
BM1580 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CDC25C Antibody (Monoclonal, DCS-193) catalog # MA1018. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDC25C Antibody (Monoclonal, DCS-193) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1018)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
DCS-193
Isotype
Mouse IgG1
Immunogen
Recombinant human Cdc25c.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1018 is reactive to CDC25C in Human
Observed Molecular Weight
25 kDa
Background of CDC25C
Cell division sycle (CDC25) gene product is a protein-tyrosine phosphatase, activates a partially purified p34 (cdc2)/cyclin B complex. The cdc25 protein also shares homology with a protein phosphatase with activity against both tyrosine and serine (and thus probably threonine) phosphate residues. CDC25C is expressed predominantly in G2 phase in healer cells. The human gene encodes a protein with a predicted molecular mass of 53,000 daltons whose C-terminal domain shares about 37% sequence identity with the fission yeast cdc25 mitotic inducer. CDC25C gene is mapped to 5q31.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1018 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1-2μg/ml, Human
Validation Images & Assay Conditions
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Boster Kit Box
Specific Publications For Anti-CDC25C Antibody (Monoclonal, DCS-193) (MA1018)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-CDC25C Antibody (Monoclonal, DCS-193)
Question
Is there a BSA free version of anti-CDC25C antibody (Monoclonal, DCS-193) MA1018 available?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CDC25C antibody (Monoclonal, DCS-193) MA1018 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-20
Question
Is a blocking peptide available for product anti-CDC25C antibody (Monoclonal, DCS-193) (MA1018)?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
We do provide the blocking peptide for product anti-CDC25C antibody (Monoclonal, DCS-193) (MA1018). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-11
Question
Can you help my question with product MA1018, anti-CDC25C antibody (Monoclonal, DCS-193). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Dhar
Verified customer
Asked: 2019-08-22
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1018 anti-CDC25C antibody (Monoclonal, DCS-193), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-22
Question
Will MA1018 anti-CDC25C antibody (Monoclonal, DCS-193) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
It shows on the product datasheet, MA1018 anti-CDC25C antibody (Monoclonal, DCS-193) as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-11
Question
I was wanting to use your anti-CDC25C antibody (Monoclonal, DCS-193) for WB for human testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human testis identification?
Verified Customer
Verified customer
Asked: 2017-05-15
Answer
As indicated on the product datasheet, MA1018 anti-CDC25C antibody (Monoclonal, DCS-193) has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-15
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-CDC25C antibody (Monoclonal, DCS-193) MA1018. Let me know if you need anything else.
H. Wu
Verified customer
Asked: 2016-02-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-02-09
Question
Is this MA1018 anti-CDC25C antibody (Monoclonal, DCS-193) reactive to the isotypes of CDC25C?
O. Jha
Verified customer
Asked: 2015-05-21
Answer
The immunogen of MA1018 anti-CDC25C antibody (Monoclonal, DCS-193) is Recombinant human Cdc25c. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-05-21
