Anti-CDC25C Antibody (Monoclonal, DCS-193)

Boster Bio Anti-CDC25C Antibody (Monoclonal, DCS-193) catalog # MA1018. Tested in WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: MA1018
Size: 100μg/vial
Reactive Species: Human
Host: Mouse
Application: WB

Product Name

Anti-CDC25C Antibody (Monoclonal, DCS-193)

See all CDC25C products

SKU/Catalog Number

MA1018

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CDC25C Antibody (Monoclonal, DCS-193) catalog # MA1018. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CDC25C Antibody (Monoclonal, DCS-193) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1018)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

DCS-193

Isotype

Mouse IgG1

Immunogen

Recombinant human Cdc25c.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1018 is reactive to CDC25C in Human

Applications

MA1018 is guaranteed for WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 1-2μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CDC25C (Source: Uniprot.Org, NCBI)

Uniprot ID

P30307

Gene ID

995

Gene Name

CDC25C

Full Name

M-phase inducer phosphatase 3

Weight

53.365kDa

Superfamily

MPI phosphatase family

Alternative Names

CDC 25; CDC25; CDC25C; cell division cycle 25 homolog C (S. cerevisiae); cell division cycle 25 homolog C (S. pombe); cell division cycle 25C; Dual specificity phosphatase Cdc25C; EC 3.1.3.48; mitosis inducer CDC25; M-phase inducer phosphatase 3; phosphotyrosine phosphatase CDC25C CDC25, PPP1R60 cell division cycle 25C M-phase inducer phosphatase 3|CDC25 homolog C|dual specificity phosphatase CDC25C|mitosis inducer CDC25|phosphotyrosine phosphatase|protein phosphatase 1, regulatory subunit 60

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CDC25C, check out the CDC25C Infographic

CDC25C infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CDC25C: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1018 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ling H, Lu Lf, He J, Xiao Gh, Jiang H, Su Q. Oncol Rep. 2014 Nov;32(5):2274-82. Doi: 10.3892/Or.2014.3417. Epub 2014 Aug 19. Diallyl Disulfide Selectively Causes Checkpoint Kinase-1 Mediated G2/M Arrest In Human Mgc803 Gastric Cancer Cell Line.

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7 Customer Q&As for Anti-CDC25C Antibody (Monoclonal, DCS-193)

Question

Is there a BSA free version of anti-CDC25C antibody (Monoclonal, DCS-193) MA1018 available?

Verified Customer

Verified customer

Asked: 2019-09-20

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CDC25C antibody (Monoclonal, DCS-193) MA1018 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-20

Question

Is a blocking peptide available for product anti-CDC25C antibody (Monoclonal, DCS-193) (MA1018)?

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

We do provide the blocking peptide for product anti-CDC25C antibody (Monoclonal, DCS-193) (MA1018). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-11

Question

Can you help my question with product MA1018, anti-CDC25C antibody (Monoclonal, DCS-193). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

A. Dhar

Verified customer

Asked: 2019-08-22

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1018 anti-CDC25C antibody (Monoclonal, DCS-193), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-22

Question

Will MA1018 anti-CDC25C antibody (Monoclonal, DCS-193) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-07-11

Answer

It shows on the product datasheet, MA1018 anti-CDC25C antibody (Monoclonal, DCS-193) as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-07-11

Question

I was wanting to use your anti-CDC25C antibody (Monoclonal, DCS-193) for WB for human testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human testis identification?

Verified Customer

Verified customer

Asked: 2017-05-15

Answer

As indicated on the product datasheet, MA1018 anti-CDC25C antibody (Monoclonal, DCS-193) has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-05-15

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-CDC25C antibody (Monoclonal, DCS-193) MA1018. Let me know if you need anything else.

H. Wu

Verified customer

Asked: 2016-02-09

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-02-09

Question

Is this MA1018 anti-CDC25C antibody (Monoclonal, DCS-193) reactive to the isotypes of CDC25C?

O. Jha

Verified customer

Asked: 2015-05-21

Answer

The immunogen of MA1018 anti-CDC25C antibody (Monoclonal, DCS-193) is Recombinant human Cdc25c. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2015-05-21

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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MA1018
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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