Anti-CDC25C Antibody (Monoclonal, DCS-193)

SKU MA1018
Size 100μg/vial
Reactivity Human
Clonality Monoclonal
Clone Number DCS-193
Host Mouse
Ig Isotype Mouse IgG1
Applications WB

Overview

Product Name Anti-CDC25C Antibody (Monoclonal, DCS-193)
SKU/Catalog Number MA1018
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for CDC25C, cell division cycle 25 homolog C (S. pombe) (CDC25C) detection. Tested with WB in Human. No cross reactivity with other proteins.
Cite This Product Anti-CDC25C Antibody (Monoclonal, DCS-193) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1018)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen Recombinant human Cdc25c.
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Western blot, 1-2μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.

Images And Assay Conditions

Boster Kit Box

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id D4ADT2
Gene Name CDC25C
Protein Name cell division cycle 25 homolog C(S. pombe)
Alternative Names Protein Cdc25c ;Cdc25c ;
Subcellular Localization CDC25C: Nucleus |Cdc25c: Nucleus.
Molecular Weight 50896 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function CDC25C: Functions as a dosage-dependent inducer in mitoticcontrol. Tyrosine protein phosphatase required for progression ofthe cell cycle. When phosphorylated, highly effective inactivating G2 cells into prophase. Directly dephosphorylates CDK1and activates its kinase activity.
Research Areas Human

*You can search these to find other products in these research areas.
Background Cell division sycle(CDC25) gene product is a protein-tyrosine phosphatase, activates a partially purified p34(cdc2)/cyclin B complex. The cdc25 protein also shares homology with a protein phosphatase with activity against both tyrosine and serine(and thus probably threonine) phosphate residues. CDC25C is expressed predominantly in G2 phase in healer cells. The human gene encodes a protein with a predicted molecular mass of 53,000 daltons whose C-terminal domain shares about 37% sequence identity with the fission yeast cdc25 mitotic inducer. CDC25C gene is mapped to 5q31.

Other Recommended Resources

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Monoclonal clone# G2 antibody for CDC25C detection. Host: Mouse.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. CDC25C information: Molecular Weight: 50896 MW; Subcellular Localization: CDC25C: Nucleus |Cdc25c: Nucleus.
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In stock
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MA1018
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

Troubleshooting

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Publications

Ling H, Lu Lf, He J, Xiao Gh, Jiang H, Su Q. Oncol Rep. 2014 Nov;32(5):2274-82. Doi: 10.3892/Or.2014.3417. Epub 2014 Aug 19. Diallyl Disulfide Selectively Causes Checkpoint Kinase-1 Mediated G2/M Arrest In Human Mgc803 Gastric Cancer Cell Line.

Customer Q&As

  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: Can you provide monoclonal antibodies carrier free?
    A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to cdc25 antibody, cdc25c antibody
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