|Ig Isotype||Mouse IgG1|
|Product Name||Anti-CDC25C Antibody (Monoclonal, DCS-193)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for CDC25C, cell division cycle 25 homolog C (S. pombe) (CDC25C) detection. Tested with WB in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-CDC25C Antibody (Monoclonal, DCS-193) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1018)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Recombinant human Cdc25c.|
Assay Dilutions Overview
Western blot, 1-2μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
Images And Assay Conditions
Boster Kit Box
Protein Target Info (Source: Uniprot.org)
|Protein Name||cell division cycle 25 homolog C(S. pombe)|
|Alternative Names||Protein Cdc25c ;Cdc25c ;|
|Subcellular Localization||CDC25C: Nucleus |Cdc25c: Nucleus.|
|Molecular Weight||50896 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||CDC25C: Functions as a dosage-dependent inducer in mitoticcontrol. Tyrosine protein phosphatase required for progression ofthe cell cycle. When phosphorylated, highly effective inactivating G2 cells into prophase. Directly dephosphorylates CDK1and activates its kinase activity.|
|Research Areas||Cell Biology, Cell Cycle, Epigenetics And Nuclear Signaling, Kinases/Phosphatases
*You can search these to find other products in these research areas.
|Background||Cell division sycle(CDC25) gene product is a protein-tyrosine phosphatase, activates a partially purified p34(cdc2)/cyclin B complex. The cdc25 protein also shares homology with a protein phosphatase with activity against both tyrosine and serine(and thus probably threonine) phosphate residues. CDC25C is expressed predominantly in G2 phase in healer cells. The human gene encodes a protein with a predicted molecular mass of 53,000 daltons whose C-terminal domain shares about 37% sequence identity with the fission yeast cdc25 mitotic inducer. CDC25C gene is mapped to 5q31.|
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