Anti-Cdc25C Antibody Picoband™

Product Name

Anti-Cdc25C Antibody Picoband™

View all CDC25C Antibodies

SKU/Catalog Number

PB9849

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cdc25C Antibody Picoband™ catalog # PB9849. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cdc25C Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9849)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Cdc25C, different from the related mouse sequence by ten amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9849 is reactive to CDC25C in Human

Applications

PB9849 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

53 kDa

Calculated molecular weight

53.365kDa

Background of CDC25C

M-phase inducer phosphatase 3 is an enzyme that in humans is encoded by the CDC25C gene. This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 (CDK1) and triggers entry into mitosis. Also, it is thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Cdc25C using anti-Cdc25C antibody (PB9849).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: SW620 whole cell lysates,
Lane 3: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc25C antigen affinity purified polyclonal antibody (Catalog # PB9849) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc25C at approximately 53 kDa. The expected band size for Cdc25C is at 53 kDa.

Gene/Protein Information For CDC25C (Source: Uniprot.org, NCBI)

Alternative Names

CDC 25; CDC25; CDC25C; cell division cycle 25 homolog C (S. cerevisiae); cell division cycle 25 homolog C (S. pombe); cell division cycle 25C; Dual specificity phosphatase Cdc25C; EC 3.1.3.48; mitosis inducer CDC25; M-phase inducer phosphatase 3; phosphotyrosine phosphatase CDC25C CDC25, PPP1R60 cell division cycle 25C M-phase inducer phosphatase 3|CDC25 homolog C|dual specificity phosphatase CDC25C|mitosis inducer CDC25|phosphotyrosine phosphatase|protein phosphatase 1, regulatory subunit 60

For more info on CDC25C, check out the CDC25C Infographic

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In this infographic, you will see the following information for CDC25C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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