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Product Info Summary
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Liquid (sterile filtered)
Boster Bio Anti-CDC27 Antibody catalog # A03905-2. Tested in IF, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Cite This Product
Anti-CDC27 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03905-2)
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids 422-430 of Human cdc27.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
A03905-2 is reactive to CDC27 in Human
A03905-2 is guaranteed for IF, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
IF Microscopy: User Optimized
Western Blot: 1:500 - 1:2,000
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CDC27 using anti-CDC27 antibody (A03905-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC27 antigen affinity purified polyclonal antibody (Catalog # A03905-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CDC27.
Gene/Protein Information For CDC27 (Source: Uniprot.org, NCBI)
Cell division cycle protein 27 homolog
ANAPC3CDC27 homolog; anaphase promoting complex subunit 3; Anaphase-promoting complex subunit 3; anaphase-promoting complex, protein 3; APC 3; APC3cell division cycle 27; CDC27Hs; cell division cycle 27 homolog (S. cerevisiae); cell division cycle protein 27 homolog; D0S1430E; D17S978E; HNUC; H-NUC; nuc2 homolog; NUC2 CDC27 ANAPC3, APC3Hs, D0S1430E, D17S978E, H-NUC, HNUC, NUC2, CDC27 cell division cycle 27 cell division cycle protein 27 homolog|D0S1430E, D17S978E|anaphase promoting complex subunit 3|anaphase-promoting complex, protein 3|cell division cycle 27 homolog|nuc2 homolog*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on CDC27, check out the CDC27 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC27: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
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