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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin).|
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Rat E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat E-Cadherin 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Cdh1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1434)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: Q24-S704
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1434 is reactive to Cdh1 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
EK1434 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Cdh1
Cadherin-1, also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin, is a protein that in humans is encoded by the CDH1 gene. E-cadherin is a Ca (2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. It plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems. The standard product used in this kit is recombinant gene expression with the molecular mass of 120KDa.
|EK1434-CAP||Anti-Rat Cdh1 Pre-coated 96-well strip microplate||1|
|EK1434-ST||Rat Cdh1 Standard||2 vials, 10 ng/tube|
|EK1434-DA||Rat Cdh1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat E-Cadherin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Cdh1, Dilution ratio of 1:10, concentration in serum is 22.4-59.7ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Cdh1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Cdh1 (Source: Uniprot.Org, NCBI)
Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin Cdh1|cadherin 1|cadherin-1|E-cadherin|epithelial cadherin|uvomorulin*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Cdh1, check out the Cdh1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cdh1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK1434 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
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