Anti-E Cadherin 1 CDH1 Antibody Picoband™ (monoclonal, 9G2)

CDH1 antibody

Boster Bio Anti-E Cadherin 1 CDH1 Antibody Picoband™ (monoclonal, 9G2) catalog # M00063-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. Cited in 10 publication(s). Independently reviewed in 1 review(s).

Product Info Summary

SKU: M00063-2
Size: 100 μg/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-E Cadherin 1 CDH1 Antibody Picoband™ (monoclonal, 9G2)

View all CDH1 Antibodies

SKU/Catalog Number

M00063-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-E Cadherin 1 CDH1 Antibody Picoband™ (monoclonal, 9G2) catalog # M00063-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-E Cadherin 1 CDH1 Antibody Picoband™ (monoclonal, 9G2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00063-2)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

9G2

Isotype

Mouse IgG1

Immunogen

E.coli-derived human E Cadherin recombinant protein (Position: A286-A703). Human E Cadherin shares 79.7% and 80.9% amino acid (aa) sequence identity with mouse and rat E Cadherin, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M00063-2 is reactive to CDH1 in Human

Applications

M00063-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Background of CDH1

CDH1 (Cadherin 1), also known as ECAD or UVO, is a protein that in humans is encoded by the CDH1 gene. Cadherin-1 is a classical member of the cadherin superfamily. By Southern analysis of DNA from a panel of mouse-human somatic cell hybrids, Mansouri et al. (1987, 1988) assigned the UVO gene to 16q (16p11-qter). Frebourg et al. (2006) found that in human embryos CDH1 is highly expressed at 4 and 5 weeks in the frontonasal prominence and at 6 weeks in the lateral and medial nasal prominences, and is therefore expressed during critical stages of lip and palate development. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For CDH1 (Source: Uniprot.org, NCBI)

Uniprot ID

P12830

Gene ID

999

Gene Name

CDH1

Full Name

Cadherin-1

Weight

97.456kDa

Alternative Names

Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin CDH1 Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO cadherin 1 cadherin-1|CAM 120/80|E-cadherin 1|cadherin 1, E-cadherin (epithelial)|cadherin 1, type 1, E-cadherin (epithelial)|calcium-dependent adhesion protein, epithelial|cell-CAM 120/80|epididymis secretory sperm binding protein|epithelial cadherin|uvomorulin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CDH1, check out the CDH1 Infographic

CDH1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CDH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

M00063-2 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Silencing of ATPase family AAA domain-containing protein 2 inhibits migration and invasion of colorectal cancer cells.

All-transretinoic acid ameliorates bleomycin-induced lung fibrosis by downregulating the TGF- β 1/Smad3 signaling pathway in rats

Effects of Homeodomain Protein CDX2 Expression on the Proliferation and Migration of Lovo Colon Cancer Cells

Li F,Zhang C,Fu L.PRR14 overexpression promotes cell growth, epithelial to mesenchymal transition and metastasis of colon cancer via the AKT pathway.PLoS One.2019 Oct 9;14(10):e0218839.doi:10.1371/journal.pone.0218839.PMID:31596887;PMCID:PMC6785111.
Species: Human,Mouse
M00063-2 usage in article: APP:WB, SAMPLE:HCT116 CELL AND RKO CELL, DILUTION:1:200

Enoch Appiah Adu-Gyamfi,Jones Lamptey,Xue-Mei Chen,Fang-Fang Li,Cong Li,Ling-Ling Ruan,Xue-Niu Yang,Tai-Hang Liu,Ying-Xiong Wang,Yu-Bin Ding,Iodothyronine deiodinase 2 (DiO2) regulates trophoblast cell line cycle, invasion and apoptosis; and its downregulation is associated with early recurrent miscarriage,Placenta,Volume 111,2021,Pages 54-68,ISSN 0143-4004,https://doi.org/10.1016/j.placenta.2021.06.004.
Species: Human
M00063-2 usage in article: APP:WB, SAMPLE:HTR-8/SVNEO CELL, DILUTION:NA

Xian-Song Wang,Li Xie,Kaiyu Zheng et al.Human Peripheral Blood-derived Mast Cells Contribute to Epithelial to Mesenchymal Transition in Bronchial Epithelial Cells in the Presence of IL-1β,29 June 2020,PREPRINT (Version 1) available at Research Square [htt
Species: Human
M00063-2 usage in article: APP:WB, SAMPLE:16-HBE CELL, DILUTION:1:100

Piao HY,Guo S,Wang Y,Zhang J.Exosome-transmitted lncRNA PCGEM1 promotes invasive and metastasis in gastric cancer by maintaining the stability of SNAI1.Clin Transl Oncol.2020 Jun 9.doi:10.1007/s12094-020-02412-9.Epub ahead of print.PMID:32519176.
Species: Human
M00063-2 usage in article: APP:WB, SAMPLE:AGS CELL AND MKN45 CELL, DILUTION:1:200

Yang Z,Zhang J,Lu D,Sun Y, Zhao X,Wang X,Zhou W,He Q,Jiang Z.Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge. Cancer Cell Int.2020 Mar 4;20:67. doi:10.1186/s1293 5-020-1150-1.PMID:32158357;PMC
Species: Human
M00063-2 usage in article: APP:WB, SAMPLE:SW480 AND HCT116 CELLS, DILUTION:NA

Detection of epithelial to mesenchymal transition in airways of a bleomycin induced pulmonary fibrosis model derived from an %u03B1-smooth muscle actin-Cre %u2026

HIF%u20101%u03B1 binding to AEG%u20101 promoter induced upregulated AEG%u20101 expression associated with metastasis in ovarian cancer

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1 Reviews For Anti-E Cadherin 1 CDH1 Antibody Picoband™ (monoclonal, 9G2)

0

Immunohistochemistry for Anti-E Cadherin 1 CDH1 Antibody

Excellent

Application Immunohistochemistry (Paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Oesophagus
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Verified customer

Submitted

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15 Customer Q&As for Anti-E Cadherin 1 CDH1 Antibody Picoband™ (monoclonal, 9G2)

Question

My boss were satisfied with the WB result of your anti-E Cadherin antibody (monoclonal, 9G2). However we have been able to see positive staining in pancreas adherens junction using this antibody. Is that expected? Could you tell me where is CDH1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-05-06

Answer

From literature, pancreas does express CDH1. Generally CDH1 expresses in cell junction, adherens junction. Regarding which tissues have CDH1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14595118
Epidermal carcinoma, Pubmed ID: 10597309
Hippocampus, and Tongue, Pubmed ID: 14702039
Liver, Pubmed ID: 3263290, 8185635, 24275569, 8033105, 11953314
Pancreas, Pubmed ID: 8459805
Placenta, Pubmed ID: 7543680, 7601454

Boster Scientific Support

Answered: 2020-05-06

Question

Is a blocking peptide available for product anti-E Cadherin antibody (monoclonal, 9G2) (M00063-2)?

Verified Customer

Verified customer

Asked: 2020-03-12

Answer

We do provide the blocking peptide for product anti-E Cadherin antibody (monoclonal, 9G2) (M00063-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-12

Question

We have tried in the past anti-E Cadherin antibody (monoclonal, 9G2) for ICC on brain last year. I am using human, and We are going to use the antibody for IF next. We need examining brain as well as epidermal carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2020-03-03

Answer

I viewed the website and datasheets of our anti-E Cadherin antibody (monoclonal, 9G2) and I see that M00063-2 has been tested on human in both ICC and IF. Thus M00063-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-03-03

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-E Cadherin antibody (monoclonal, 9G2) M00063-2. Let me know if you need anything else.

G. Walker

Verified customer

Asked: 2020-02-13

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-13

Question

Does M00063-2 anti-E Cadherin antibody (monoclonal, 9G2) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

T. Moore

Verified customer

Asked: 2019-09-30

Answer

As indicated on the product datasheet, M00063-2 anti-E Cadherin antibody (monoclonal, 9G2) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-30

Question

Is this M00063-2 anti-E Cadherin antibody (monoclonal, 9G2) reactive to the isotypes of CDH1?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

The immunogen of M00063-2 anti-E Cadherin antibody (monoclonal, 9G2) is E.coli-derived human E Cadherin recombinant protein (Position: A286-A703). Human E Cadherin shares 79.7% and 80.9% amino acid (aa) sequence identity with mouse and rat E Cadherin, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-08-15

Question

Do you have a BSA free version of anti-E Cadherin antibody (monoclonal, 9G2) M00063-2 available?

C. Gonzalez

Verified customer

Asked: 2019-06-21

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-E Cadherin antibody (monoclonal, 9G2) M00063-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-06-21

Question

We are currently using anti-E Cadherin antibody (monoclonal, 9G2) M00063-2 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-17

Answer

The anti-E Cadherin antibody (monoclonal, 9G2) (M00063-2) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-17

Question

My question regards to test anti-E Cadherin antibody (monoclonal, 9G2) M00063-2 on human brain for research purposes, then I may be interested in using anti-E Cadherin antibody (monoclonal, 9G2) M00063-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-01-25

Answer

The products we sell, including anti-E Cadherin antibody (monoclonal, 9G2) M00063-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-01-25

Question

Will anti-E Cadherin antibody (monoclonal, 9G2) M00063-2 work for WB with brain?

G. Huang

Verified customer

Asked: 2017-08-22

Answer

According to the expression profile of brain, CDH1 is highly expressed in brain. So, it is likely that anti-E Cadherin antibody (monoclonal, 9G2) M00063-2 will work for WB with brain.

Boster Scientific Support

Answered: 2017-08-22

Question

I have attached the WB image, lot number and protocol we used for brain using anti-E Cadherin antibody (monoclonal, 9G2) M00063-2. Please let me know if you require anything else.

B. Wu

Verified customer

Asked: 2017-06-29

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-06-29

Question

My question regarding product M00063-2, anti-E Cadherin antibody (monoclonal, 9G2). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

K. Lewis

Verified customer

Asked: 2016-09-09

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00063-2 anti-E Cadherin antibody (monoclonal, 9G2), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-09-09

Question

I see that the anti-E Cadherin antibody (monoclonal, 9G2) M00063-2 works with WB, what is the protocol used to produce the result images on the product page?

L. Jones

Verified customer

Asked: 2015-09-21

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-09-21

Question

I was wanting to use your anti-E Cadherin antibody (monoclonal, 9G2) for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?

J. Anderson

Verified customer

Asked: 2013-07-02

Answer

It shows on the product datasheet, M00063-2 anti-E Cadherin antibody (monoclonal, 9G2) has been tested for Flow Cytometry, IF, IHC-P, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-07-02

Question

We have seen staining in human liver. Do you have any suggestions? Is anti-E Cadherin antibody (monoclonal, 9G2) supposed to stain liver positively?

C. Li

Verified customer

Asked: 2013-02-15

Answer

From what I have seen in literature liver does express CDH1. From what I have seen in Uniprot.org, CDH1 is expressed in jejunal mucosa, pancreas, liver, epidermal carcinoma, hippocampus tongue, placenta, brain, among other tissues. Regarding which tissues have CDH1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14595118
Epidermal carcinoma, Pubmed ID: 10597309
Hippocampus, and Tongue, Pubmed ID: 14702039
Liver, Pubmed ID: 3263290, 8185635, 24275569, 8033105, 11953314
Pancreas, Pubmed ID: 8459805
Placenta, Pubmed ID: 7543680, 7601454

Boster Scientific Support

Answered: 2013-02-15

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Total: $315

SKU:M00063-2

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M00063-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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