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- Table of Contents
CDH1 antibody (M00063-2) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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Flow Cytometry analysis of A549 cells using anti- CA2 antibody (M00063-2).
Overlay histogram showing A549 cells stained with M00063-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CA2 Antibody (M00063-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A549 cells stained with M00063-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CA2 Antibody (M00063-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2)
CDH1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
CDH1 antibody was positively detected by WB in:
human placenta tissue, human A549 whole cell, human HEK293 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CDH1 antibody was positively detected by IHC in:
human prostate cancer tissue, human liver cancer tissue, human placenta tissue, human colorectal adenocarcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CDH1 antibody was positively detected by ICC/IF in:
A431 cell
CDH1 antibody was positively detected by IF in:
human colon cancer tissue, human intestinal cancer tissue
CDH1 antibody was positively detected by FC in:
A549 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunocytochemistry/Immunofluorescence
2μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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Boster Guarantee:What is this?
WB
IHC
IF
Human
Tested
Tested
Tested
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2)
View all CDH1 Antibodies
View all CDH1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
9G2
Isotype
Mouse IgG1
Immunogen
E.coli-derived human E Cadherin recombinant protein (Position: A286-A703). Human E Cadherin shares 79.7% and 80.9% amino acid (aa) sequence identity with mouse and rat E Cadherin, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
130 kDa
Calculated Molecular Weight
97.5 kDa
Background of CDH1
CDH1 (Cadherin 1), also known as ECAD or UVO, is a protein that in humans is encoded by the CDH1 gene. Cadherin-1 is a classical member of the cadherin superfamily. By Southern analysis of DNA from a panel of mouse-human somatic cell hybrids, Mansouri et al. (1987, 1988) assigned the UVO gene to 16q (16p11-qter). Frebourg et al. (2006) found that in human embryos CDH1 is highly expressed at 4 and 5 weeks in the frontonasal prominence and at 6 weeks in the lateral and medial nasal prominences, and is therefore expressed during critical stages of lip and palate development. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
Cite This Product
Anti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00063-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
94%
High Homology
Rabbit
83%
Moderate Homology
Cow
81%
Moderate Homology
Rat
81%
Moderate Homology
Sheep
80%
Moderate Homology
Mouse
80%
Moderate Homology
Dog
79%
Moderate Homology
Zebrafish
49%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CDH1 antibody
Immunohistochemistry (IHC) protocol for CDH1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CDH1 antibody
Immunofluorescence (IF) protocol for CDH1 antibody
Flow Cytometry (FC) protocol for CDH1 antibody
Publications
Application:
Species:
Stretchable Sensor-Integrated Microfluidic Chip for Monitoring Fatty Acid-Enhanced Serotonin Release from the Intestinal Epithelium
Published: 2025-09-08
Journal: ANALYTICAL CHEMISTRY
Application: IF
Impact Factor: 6.70
Species: Human
PMID: 40920399
Expression and potential molecular mechanism of TOP2A in metastasis of non-small cell lung cancer
Published: 2024-05-28
Journal: Scientific Reports
Application: WB
Impact Factor: 3.80
Species: Human
PMID: 38806610
Low PDE4A expression promoted the progression of ovarian cancer by inducing Snail nuclear translocation
Published: 2024-05-24
Journal: EXPERIMENTAL CELL RESEARCH
Application: WB,IF
Impact Factor: 3.30
Species: Human,Mouse
PMID: 38797258
Engineering HepG2 spheroids with injectable fiber fragments as predictable models for drug metabolism and tumor infiltration
Published: 2020-07-05
Journal: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
Application: IHC
Impact Factor: 2.83
Species: Human
PMID: 32627303
Migration of gastric cancer is suppressed by recombinant Newcastle disease virus (rL-RVG) via regulating α7-nicotinic acetylcholine receptors/ERK- EMT
Published: 2019-10-22
Journal: BMC CANCER
Application: WB
Impact Factor: 2.93
Species: Human
PMID: 31640627
Hypoxia-induced LncRNA PCGEM1 promotes invasion and metastasis of gastric cancer through regulating SNAI1
Published: 2019-01-28
Journal: Clinical & Translational Oncology
Application: WB
Impact Factor: 2.44
Species: Human
PMID: 30690667
Silencing of ATPase family AAA domain-containing protein 2 inhibits migration and invasion of colorectal cancer cells.
Published: 2016-08-28
Journal: NEOPLASMA
Application: WB
Impact Factor: 1.96
Species: Human
PMID: 27565322
Effect of grape seed proanthocyanidins on tumor vasculogenic mimicry in human triple-negative breast cancer cells.
Published: 2015-01-01
Journal: Asian Pacific Journal of Cancer Prevention
Application: WB
Impact Factor: 2.51
Species: Human
All-transretinoic acid ameliorates bleomycin-induced lung fibrosis by downregulating the TGF-β1/Smad3 signaling pathway in rats
Published: 2013-09-16
Journal: LABORATORY INVESTIGATION
Application: IF
Impact Factor: 3.96
Species: Rat
PMID: 24042439
Effects of Homeodomain Protein CDX2 Expression on the Proliferation and Migration of Lovo Colon Cancer Cells
Published: 2011-04-07
Journal: PATHOLOGY & ONCOLOGY RESEARCH
Application: ICC
Impact Factor: 1.48
Species: Human
PMID: 21626348
Review
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1 Review For Anti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2)
Immunohistochemistry for Anti-E Cadherin 1 CDH1 Antibody
| SKU | M00063-2 |
|---|---|
| Application | Immunohistochemistry (Paraffin-embedded) |
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Human Oesophagus |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 4°C overnight |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 2ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | at 37°C for 30 min |
K. Evans
Verified customer
Submitted 2020-07-16
Product Promise
We promise Anti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2) performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00063-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026