Product Info Summary
SKU: | M00356-3 |
---|---|
Size: | 100uL |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CEA (CD66e) Rabbit Monoclonal Antibody, Clone#RM326
View all CEACAM5/CD66e Antibodies
SKU/Catalog Number
M00356-3
Size
100uL
Form
Liquid
Description
Boster Bio Anti-CEA (CD66e) Rabbit Monoclonal Antibody, Clone#RM326 (Catalog # M00356-3). Tested in IHC, WB, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CEA (CD66e) Rabbit Monoclonal Antibody, Clone#RM326 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00356-3)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM326
Isotype
Rabbit IgG
Immunogen
Native CEA protein purified from human liver tissue.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human CEA (Carcinoembryonic Antigen) (CD66e).
Reactive Species
M00356-3 is reactive to CEACAM5 in Human
Applications
M00356-3 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
76.795kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:1000-1:2000 dilution
WB: 1:1000-1:2000 dilution
ELISA: 1:1000-1:5000 dilution.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western Blotting result
Western Blot of MCF-7 cell lysate using anti-CEA rabbit monoclonal antibody (Clone RM326) at a 1:1000 dilution.
Click image to see more details
Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human colon tissue section using anti-CEA rabbit monoclonal antibody (Clone RM326) at a 1:2000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CEACAM5 (Source: Uniprot.org, NCBI)
Gene Name
CEACAM5
Full Name
Carcinoembryonic antigen-related cell adhesion molecule 5
Weight
76.795kDa
Superfamily
immunoglobulin superfamily
Alternative Names
Carcinoembryonic antigen; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e antigen; CD66e; CEA; CEACAM5; CEACAM-5; CEACD66e; DKFZp781M2392; Meconium antigen 100 CEACAM5 CD66e, CEA CEA cell adhesion molecule 5 carcinoembryonic antigen-related cell adhesion molecule 5|carcinoembryonic antigen related cell adhesion molecule 5|meconium antigen 100
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CEACAM5, check out the CEACAM5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CEACAM5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CEA (CD66e) Rabbit Monoclonal Antibody, Clone#RM326 (M00356-3)
Hello CJ!
No publications found for M00356-3
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CEA (CD66e) Rabbit Monoclonal Antibody, Clone#RM326?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-CEA (CD66e) Rabbit Monoclonal Antibody, Clone#RM326
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-CEA (CD66e) Rabbit Monoclonal Antibody, Clone#RM326
Question
I was wanting to use your anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 for WB for human colonic mucosa on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human colonic mucosa identification?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
It shows on the product datasheet, M00356-3 anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 has been tested for ELISA, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human colonic mucosa in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-18
Question
Is a blocking peptide available for product anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 (M00356-3)?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
We do provide the blocking peptide for product anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 (M00356-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-20
Question
Is there a BSA free version of anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 M00356-3 available?
J. Gonzalez
Verified customer
Asked: 2020-01-15
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 M00356-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-15
Question
My colleagues were happy with the WB result of your anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
From what I have seen in literature, colonic mucosa does express CEACAM5. Generally CEACAM5 expresses in cell membrane. Regarding which tissues have CEACAM5 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Saliva, Pubmed ID: 16740002
Boster Scientific Support
Answered: 2020-01-14
Question
Please see the WB image, lot number and protocol we used for colonic mucosa using anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 M00356-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-26
Question
We are currently using anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 M00356-3 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
The anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 (M00356-3) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-27
Question
I am interested in to test anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 M00356-3 on human colonic mucosa for research purposes, then I may be interested in using anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 M00356-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
The products we sell, including anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 M00356-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-16
Question
We have seen staining in human liver. Are there any suggestions? Is anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
From what I have seen in literature liver does express CEACAM5. From what I have seen in Uniprot.org, CEACAM5 is expressed in colonic mucosa, saliva, liver, among other tissues. Regarding which tissues have CEACAM5 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Saliva, Pubmed ID: 16740002
Boster Scientific Support
Answered: 2019-07-29
Question
We ordered your anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
I have checked the website and datasheets of our anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2019-05-21
Question
Will anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 M00356-3 work for WB with colonic mucosa?
Verified Customer
Verified customer
Asked: 2018-01-16
Answer
According to the expression profile of colonic mucosa, CEACAM5 is highly expressed in colonic mucosa. So, it is likely that anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 M00356-3 will work for WB with colonic mucosa.
Boster Scientific Support
Answered: 2018-01-16
Question
Would M00356-3 anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Huang
Verified customer
Asked: 2017-10-23
Answer
It shows on the product datasheet, M00356-3 anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-23
Question
you antibody using your anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 for apoptotic process studies. Has this antibody been tested with western blotting on human colon tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-09-26
Answer
Thanks for your inquiry. This M00356-3 anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 is tested on human colon tissue. It is guaranteed to work for ELISA, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-09-26
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colonic mucosa using anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 M00356-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-08-23
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-23
Question
Is this M00356-3 anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 reactive to the isotypes of CEACAM5?
L. Jones
Verified customer
Asked: 2016-12-29
Answer
The immunogen of M00356-3 anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 is Native CEA protein purified from human liver tissue. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-12-29
Question
I see that the anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326 M00356-3 works with WB, what is the protocol used to produce the result images on the product page?
A. Krishna
Verified customer
Asked: 2016-03-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-03-21
Question
I have a question about product M00356-3, anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Jha
Verified customer
Asked: 2014-12-19
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00356-3 anti-CEA (CD66e) Rabbit Monoclonal antibody, Clone#RM326, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-12-19