Rabbit IgG polyclonal antibody for Carcinoembryonic antigen-related cell adhesion molecule 5(CEACAM5) detection. Tested with WB, IHC-P, ELISA in Human.
Anti-CEACAM5 Antibody Info At A Glance
|Applications:||ELISA, IHC, WB|
|Product Name||Anti-CEACAM5 Antibody
See all CEACAM5/CD66e primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for CD66e/CEACAM5 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Human. CD66e/CEACAM5 information: Molecular Weight: 76795 MW; Subcellular Localization: Cell membrane ; Lipid-anchor, GPI-anchor ; Tissue Specificity: Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.|
|Cite This Product||Anti-CEACAM5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1018)|
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|Anti-CEACAM5 Antibody may replace the following items: sc 23928|sc 59873|sc 59872|sc 52390.|
|Applications||ELISA, IHC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||Insect cells-derived human CEA recombinant protein(Position: full).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For CEACAM5 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||1048|
|Species Of This Entry||Human|
|Protein Name||Carcinoembryonic antigen-related cell adhesion molecule 5|
|Alternative Names||CEACAM5/CD66e|Carcinoembryonic antigen; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e antigen; CD66e; CEA; CEACAM5; CEACAM-5; CEACD66e; DKFZp781M2392; Meconium antigen 100|
|Post Tranlational Modifications||Glycosylation; Cleavage; Gpi Anchoring|
|Subcellular Localization||Cell membrane; Lipid-anchor, GPI-anchor. Apical cell membrane. Cell surface. Localized to the apical glycocalyx surface.|
|Gene Location||19q13.2, on Chromasome 19, gene sequence: NC_000019.10 (41708626..41730433)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CEACAM5 (Source: Uniprot.org, NCBI)
|Protein Function||Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed:2803308, PubMed:10910050, PubMed:10864933). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells (PubMed:10910050).|
|Tissue Specificity||Expressed in columnar epithelial and goblet cells of the colon (at protein level) (PubMed:10436421). Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.|
|Related Diseases||Malignant Neoplasms; Neoplasms; Carcinoma; Tissue Adhesions; Malignant Tumor Of Colon; Colorectal Cancer; Colorectal Neoplasms; Neoplasm Metastasis; Adenocarcinoma; Colonic Neoplasms; Cell Invasion; Lymphatic Metastasis; Malignant Neoplasm Of Breast; Mammary Neoplasms; Lung Neoplasms; Malignant Paraganglionic Neoplasm; Uterine Cervical Neoplasm; Malignant Neoplasm Of Lung; Malignant Tumor Of Cervix|
|Related Pathways||Cell Adhesion; Cell Migration; Immune Response; Cell Proliferation; Cell Growth; Antibody-dependent Cellular Cytotoxicity; Glycosylation; Secretion; Dna Integration; Proteolysis; Reverse Transcription; Phagocytosis; Cell Motility; Cell Cycle; Contact Inhibition; Cell-cell Adhesion; Cell Killing; Complement-dependent Cytotoxicity; Localization; Mismatch Repair|
|Background||CEA(Carcinoembryonic antigen) is a glycoprotein involved in cell adhesion. It is normally produced during fetal development, but the production of CEA stops before birth. Therefore, it is not usually present in the blood of healthy adults, although levels are raised in heavy smokers. CEA is a glycosyl phosphatidyl inositol(GPI)-cell surface anchored glycoprotein whose specialized sialofucosylated glycoforms serve as functional colon carcinoma L-selectin and E-selectin ligands, which may be critical to the metastatic dissemination of colon carcinoma cells. CEA and related genes make up the CEA family belonging to the immunoglobulin superfamily. In humans, the carcinoembryonic antigen family consists of 29 genes, 18 of which are normally expressed.|
|Scientific References||PMID: 3670312 by Beauchemin N., et al. Isolation and characterization of full-length functional cDNA clones for human carcinoembryonic antigen.|
PMID: 3220478 by Barnett T., et al. Carcinoembryonic antigen family: characterization of cDNAs coding for NCA and CEA and suggestion of nonrandom sequence variation in their conserved loop-domains.
Our Boster Quality Guarantee for Anti-CEACAM5 Antibody covers its use in the following applications.ELISA , 0.1-0.5μg/ml, Human, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SW620 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: mouse small intestine tissue lysates,
Lane 4: mouse stomach tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse NIH3T3 whole cell lysates,
Lane 8: mouse HEPA1-6 whole cell lysates,
Lane 9: mouse SP20 whole cell lysates,
Lane 10: rat RH35 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CEA antigen affinity purified polyclonal antibody (Catalog # RP1018) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEA at approximately 120-200KD. The expected band size for CEA is at 77KD.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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