|Application:||ELISA, IHC, WB|
Data & Images
|Product Name||Anti-CEA Antibody|
|Description||Rabbit IgG polyclonal antibody for Carcinoembryonic antigen-related cell adhesion molecule 5(CEACAM5) detection. Tested with WB, IHC-P, ELISA in Human.|
|Cite This Product||Anti-CEA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1018)|
|Replacement Item||This antibody may replace the following items: sc-23928|sc-59873|sc-59872|sc-52390 from Santa Cruz Biotechnology.|
|Application||ELISA, IHC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Insect cells-derived human CEA recombinant protein(Position: full).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Carcinoembryonic antigen-related cell adhesion molecule 5|
|Molecular Weight||76795 MW|
|Protein Function||Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. .|
|Tissue Specificity||Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.|
|Sequence Similarities||Belongs to the immunoglobulin superfamily. CEA family.|
|Subcellular Localization||Cell membrane ; Lipid-anchor, GPI-anchor .|
|Alternative Names||Carcinoembryonic antigen-related cell adhesion molecule 5;Carcinoembryonic antigen;CEA;Meconium antigen 100;CD66e;CEACAM5;CEA;|
|Research Areas|||tags & cell markers|cell type markers|tumor associated| cancer|tumor immunology|tumor-associated antigens|oncoproteins/suppressors|tumor suppressors|tumor biomarkers|tumor antigens| microbiology|protein|human protein|receptor||
Background for Carcinoembryonic antigen-related cell adhesion molecule 5
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CEA Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
ELISA , 0.1-0.5μg/ml, Human, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CEA Antibody Images
Click the images to enlarge.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SW620 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: mouse small intestine tissue lysates,
Lane 4: mouse stomach tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse NIH3T3 whole cell lysates,
Lane 8: mouse HEPA1-6 whole cell lysates,
Lane 9: mouse SP20 whole cell lysates,
Lane 10: rat RH35 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CEA antigen affinity purified polyclonal antibody (Catalog # RP1018) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEA at approximately 120-200KD. The expected band size for CEA is at 77KD.
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,