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Product Info Summary
| SKU: | EK0904 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human CEA / Carcino Embryonic Antigen ELISA Kit PicoKine®
SKU/Catalog Number
EK0904
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CEA / Carcino Embryonic Antigen ELISA Kit PicoKine® (96 Tests). Quantitate Human CEACAM5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CEA / Carcino Embryonic Antigen ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0904)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: K35-A685
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0904 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0904 is reactive to CEACAM5 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0904 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CEACAM5
Carcinoembryonic antigen (CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0904-CAP | Anti-Human CEACAM5 Pre-coated 96-well strip microplate | 1 |
| EK0904-ST | Human CEACAM5 Standard | 2 vials, 20 ng/tube |
| EK0904-DA | Human CEACAM5 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.312 | 0.62 | 1.25 | 2.5 | 5 | 10 | 20 |
| O.D. | 0.041 | 0.089 | 0.132 | 0.281 | 0.432 | 0.87 | 1.334 | 2.05 |
Data Example Images
Click image to see more details
Human CEA PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CEACAM5, Dilution ratio of 1:1, concentration in serum and plasma is around 400 pg/ml..
Some articles we found to cite concentrations of CEACAM5 in samples: 24990528, 23886200 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CEA / Carcino Embryonic Antigen ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 776 | 1668 | 10176 | 724 | 1662 | 10501 |
| Standard deviation | 39.57 | 124 | 407.04 | 39.09 | 137.94 | 420.04 |
| CV (%) | 4% | 7.5% | 4% | 5.4% | 8.3% | 4% |
Specific Publications For Human CEA / Carcino Embryonic Antigen ELISA Kit PicoKine® (EK0904)
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Human CEA / Carcino Embryonic Antigen ELISA Kit PicoKine®
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-10-17
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-10-17
Question
Q: can I use citrate plasma as samples in Human CD66e Picokine® ELISA Kit (Catalog # EK0904)?
Verified Customer
Verified customer
Asked: 2020-07-19
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of CD66e causing degradation of its protein structure. CD66e may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CD66e ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-07-19
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
P. Jha
Verified customer
Asked: 2020-05-26
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-05-26
Question
Q: What is the optimal O.D. value for CD66e ELISA kit? I used your CD66e ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CD66e even though the O.D. values are not very high?
S. Kumar
Verified customer
Asked: 2019-07-04
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD66e ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CD66e that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-07-04
Question
Q: how can I thaw whole blood sample for CD66e ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-04-26
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD66e for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-04-26
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CD66e in Human serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
M. Krishna
Verified customer
Asked: 2018-11-01
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD66e ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/elisa-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-11-01
Question
Q: how to proceed with the analysis of ELISA data? I measured CD66e level in serum.
Verified Customer
Verified customer
Asked: 2018-07-20
Answer
A: please read this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2018-07-20
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-04-17
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-04-17
Question
Can EK0904 measure human CEA levels from plasma containing formaldehyde?
Verified Customer
Verified customer
Asked: 2015-04-20
Answer
Our technicians have not worked on plasma samples containing formaldehyde, but they suspect that EK0904 will not be suitable for this type of sample.
Boster Scientific Support
Answered: 2015-04-20
Question
Is it possible to remove the final solution into separate cuvettes using a micropipette so that the absorbance can be measured using a spectrometer which does not handle microplates? Keyword: detection, protocol
Verified Customer
Verified customer
Asked: 2014-09-01
Answer
This method is not recommended. It may cause errors when removing the final solution into separate cuvettes, and the color will decay with time, O.D. absorbance at 450nm of different wells should be read at the same time.
Boster Scientific Support
Answered: 2014-09-01
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification CD66e using the EK0904 Human CD66e Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2014-02-24
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2014-02-24
