Human CEA / Carcino Embryonic Antigen ELISA Kit PicoKine™

Human CEA / Carcino Embryonic Antigen ELISA Kit PicoKine™ (96 Tests). Quantitate Human CEACAM5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 4 publication(s).

Product Info Summary

SKU: EK0904
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human CEA / Carcino Embryonic Antigen ELISA Kit PicoKine™

See all CEACAM5/CD66e products

SKU/Catalog Number

EK0904

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CEA / Carcino Embryonic Antigen ELISA Kit PicoKine™ (96 Tests). Quantitate Human CEACAM5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human CEA / Carcino Embryonic Antigen ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0904)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: K35-A685

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0904 is reactive to CEACAM5 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0904 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0904-CAP Anti-Human CEACAM5 Pre-coated 96-well strip microplate 1
EK0904-ST Human CEACAM5 Standard 2 vials, 20 ng/tube
EK0904-DA Human CEACAM5 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251250250050001000020000
O.D.0.0110.0560.1110.2100.3810.7131.2961.967

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect CEACAM5/CD66e, Dilution ratio of 1:1, concentration in serum is around 400pg/ml.

Some articles we found to cite concentrations of CEACAM5/CD66e in samples: 24990528, 23886200 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CEA / Carcino Embryonic Antigen ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)776166810176724166210501
Standard deviation39.57124407.0439.09137.94420.04
CV (%)4%7.5%4%5.4%8.3%4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CEACAM5/CD66e in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 177681677576278220.252.5%
Sample 21668175217231646169742.252.4%
Sample 3101769323941895979628331.053.4%
*number of samples for each test n=16.

Gene/Protein Information For CEACAM5 (Source: Uniprot.Org, NCBI)

Uniprot ID

P06731

Gene ID

1048

Gene Name

CEACAM5

Full Name

Carcinoembryonic antigen-related cell adhesion molecule 5

Weight

76.795kDa

Superfamily

immunoglobulin superfamily

Alternative Names

Carcinoembryonic antigen; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e antigen; CD66e; CEA; CEACAM5; CEACAM-5; CEACD66e; DKFZp781M2392; Meconium antigen 100 CEACAM5 CD66e, CEA CEA cell adhesion molecule 5 carcinoembryonic antigen-related cell adhesion molecule 5|carcinoembryonic antigen related cell adhesion molecule 5|meconium antigen 100

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CEACAM5, check out the CEACAM5 Infographic

CEACAM5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CEACAM5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0904 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Elhelbawy NG,Zaid IF,Khalifa AA,Gohar SF,Fouda EA.miRNA-148a and miRNA-30c expressions as potential biomarkers in breast cancer patients.Biochem Biophys Rep.2021 Jun 21;27:101060.doi:10.1016/j.bbrep.2021.101060.PMID:34195390;PMCID:PMC8239439.
Species: Human
EK0904 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Mona S.Habieb,Nesreen G.Elhelbawy,Alshimaa M.Alhanafy,Mohammad G.Elhelbawy,Ahmed S.Alkelany,Amany M.Wahb,Study of the potential association of the BCHE rs1803274 genetic polymorphism and serum level of its protein with breast cancer,Meta Gene,Volume 29,2021,100913,ISSN 2214-5400,https://doi.org/10.1016/j.mgene.2021.100913.
Species: Human
EK0904 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Yan-Li Zhu,Yan-Mei Lian,Ji-Kai Wang,Zeng-Ping Chen,Ru-Qin Yu,Ultrasensitive detection of protein biomarkers by MALDI-TOF mass spectrometry based on ZnFe2O4 nanoparticles and mass tagging signal amplifica tion, Talanta, 2020,121848,ISSN 0039 9140,https://d
Species: Human
EK0904 usage in article: APP:ELISA, SAMPLE:HUMAN SERUM, DILUTION:NA

Hu C, Zheng J, Su X, Wang J, Wu W, Hu S. Anal Chem. 2013 Nov 5;85(21):10612-9. Doi: 10.1021/Ac4028005. Epub 2013 Oct 11. Ultrasensitive All-Carbon Photoelectrochemical Bioprobes For Zeptomole Immunosensing Of Tumor Markers By An Inexpensive Visibl...

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11 Customer Q&As for Human CEA / Carcino Embryonic Antigen ELISA Kit PicoKine™

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-10-17

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-10-17

Question

Q: can I use citrate plasma as samples in Human CD66e Picokine® ELISA Kit (Catalog # EK0904)?

Verified Customer

Verified customer

Asked: 2020-07-19

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of CD66e causing degradation of its protein structure. CD66e may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CD66e ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-07-19

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

P. Jha

Verified customer

Asked: 2020-05-26

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-05-26

Question

Q: What is the optimal O.D. value for CD66e ELISA kit? I used your CD66e ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CD66e even though the O.D. values are not very high?

S. Kumar

Verified customer

Asked: 2019-07-04

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD66e ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CD66e that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2019-07-04

Question

Q: how can I thaw whole blood sample for CD66e ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-04-26

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD66e for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-04-26

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CD66e in Human serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

M. Krishna

Verified customer

Asked: 2018-11-01

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD66e ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-11-01

Question

Q: how to proceed with the analysis of ELISA data? I measured CD66e level in serum.

Verified Customer

Verified customer

Asked: 2018-07-20

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-07-20

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-04-17

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-04-17

Question

Can EK0904 measure human CEA levels from plasma containing formaldehyde?

Verified Customer

Verified customer

Asked: 2015-04-20

Answer

Our technicians have not worked on plasma samples containing formaldehyde, but they suspect that EK0904 will not be suitable for this type of sample.

Boster Scientific Support

Answered: 2015-04-20

Question

Is it possible to remove the final solution into separate cuvettes using a micropipette so that the absorbance can be measured using a spectrometer which does not handle microplates? Keyword: detection, protocol

Verified Customer

Verified customer

Asked: 2014-09-01

Answer

This method is not recommended. It may cause errors when removing the final solution into separate cuvettes, and the color will decay with time, O.D. absorbance at 450nm of different wells should be read at the same time.

Boster Scientific Support

Answered: 2014-09-01

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification CD66e using the EK0904 Human CD66e Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2014-02-24

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2014-02-24

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