Human Trail ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human Trail ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human TNFSF10 in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 1 pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EZ0532
Size: For 5 plates, 96 wells each
Reactive Species: Human
Application: ELISA

Product Name

Human Trail ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all TRAIL/TNFSF10 products

SKU/Catalog Number

EZ0532

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Human Trail ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human TNFSF10 in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 1 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Trail ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0532)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: T95-G281

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0532 is reactive to TNFSF10 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva.

Application Guarantee

EZ0532 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ0532-CA Rabbit anti- human TNFSF10 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ0532-DA Biotinylated goat anti- human TNFSF10 polyclonal antibody (Detection Antibody) 500 µl, 10 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ0532-ST Lyophilized recombinant human TNFSF10 standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0660.1230.1660.3030.5090.8241.5492.458

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3818441634175377
Standard deviation2.669.5618.722.759.6218.85
CV (%)7%5.2%4.5%8.1%5.5%5%

Gene/Protein Information For TNFSF10 (Source: Uniprot.Org, NCBI)

Uniprot ID

P50591

Gene ID

8743

Gene Name

TNFSF10

Full Name

Tumor necrosis factor ligand superfamily member 10

Weight

32.509kDa

Superfamily

tumor necrosis factor family

Alternative Names

Apo-2 ligand; APO2L; Apo-2Ltumor necrosis factor (ligand) family, member 10; APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta; CD253 antigen; CD253; Protein TRAIL; TL2; TNF-related apoptosis-inducing ligand; TNFSF10; TRAIL; TRAILTNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10 TNFSF10 APO2L, Apo-2L, CD253, TL2, TNLG6A, TRAIL TNF superfamily member 10 tumor necrosis factor ligand superfamily member 10|Apo-2 ligand|TNF-related apoptosis inducing ligand TRAIL|chemokine tumor necrosis factor ligand superfamily member 10|tumor necrosis factor (ligand) family, member 10|tumor necrosis factor (ligand) superfamily, member 10|tumor necrosis factor apoptosis-inducing ligand splice variant delta|tumor necrosis factor ligand 6A|tumor necrosis factor superfamily member 10

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TNFSF10, check out the TNFSF10 Infographic

TNFSF10 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TNFSF10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EZ0532 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

A novel anti-GD2/4-1BB chimeric antigen receptor triggers neuroblastoma cell killing

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