Anti-CEBP Delta Antibody

Product Name

Anti-CEBP Delta Antibody

View all CEBPD Antibodies

SKU/Catalog Number

A03499-2

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-CEBP Delta Antibody (Catalog # A03499-2). Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-CEBP Delta Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03499-2)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of mouse C/EBP.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A03499-2 is reactive to CEBPD in Mouse

Observed Molecular Weight

42 kDa

Calculated molecular weight

28.6 kDa

Background of CEBPD

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. C/EBP proteins are DNA-binding proteins that are important transcriptional activators in the regulation of genes involved in immune and inflammatory responses and may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages. Members of the C/EBP transcription factor family are related by a high degree of amino acid sequence identity to the basic leucine zipper DNA-binding domain and show distinct but overlapping patterns of tissue- and stage-restricted expression. Osada et al. identified the consensus binding site for the family as RTTGCGYAAY (R = A or G, and Y = C or T). Phosphorylation of C/EBPd may increase binding activity, whereas phosphorylation of the a and b family members may decrease binding affinity. C/EBPd is a nuclear protein that binds DNA as a dimer and can form stable heterodimers with all other C/EBP family members.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03499-2 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:4,000 - 1:9,000
IHC: 1:2000
IP: 0.8 – 1.5 µg/ml
WB: 1:1,000 - 1:15,000
This affinity purified antibody has been tested for use in ELISA, western blotting, and suitable in IHC.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 35 kDa in size corresponding to C/EBPd by western blotting in the appropriate cell lysate or extract.   The predicted molecular weight of mouse C/EBPd is 28.8 kDa. This antibody is capable of detecting both over-expressed and endogenous C/EBPd.

Validation Images & Assay Conditions

See full target information CEBPD

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