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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
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Rat SEMA6A/Semaphorin-6A ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat SEMA6A ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Sema6a in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Rat SEMA6A/Semaphorin-6A ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2057)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: G19-T649
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK2057 is reactive to Sema6a in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
EK2057 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK2057-CAP||Anti-Rat Sema6a Pre-coated 96-well strip microplate||1|
|EK2057-ST||Rat Sema6a Standard||2 vials, 10 ng/tube|
|EK2057-DA||Rat Sema6a Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat SEMA6A PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat SEMA6A/Semaphorin-6A ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Semaphorin 6A in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Sema6a (Source: Uniprot.Org, NCBI)
KIAA1368HT018; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A; Sema VIA; SEMA; Sema6A; SEMA6A1; SEMA6A-1; Semaphorin 6A; semaphorin 6A-1; Semaphorin VIA; semaphorin-6A; semaphorin-6A-1; SEMAQ; VIA Sema6a|A730020P05Rik, AI851735, Sem, Sema6, Sema6A-1, Semaq, VIa, b2b997C, se|sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A|semaphorin-6A|Mutant line 997|sema Q|sema VIA|semaphorin Q|semaphorin VIA|semaphorin-6A-1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Sema6a, check out the Sema6a Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Sema6a: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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