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Anti-Cenexin-1 ODF2 Antibody
Liquid (sterile filtered)
Boster Bio Anti-Cenexin-1 ODF2 Antibody catalog # A05599. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Cite This Product
Anti-Cenexin-1 ODF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05599)
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a truncated recombinant protein hCenexin1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
A05599 is reactive to ODF2 in Human
A05599 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western Blot: 1:500-1:2000
Other Dilution: User Optimized
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ODF2 using anti-ODF2 antibody (A05599).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ODF2 antigen affinity purified polyclonal antibody (Catalog # A05599) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for ODF2.
Gene/Protein Information For ODF2 (Source: Uniprot.org, NCBI)
Outer dense fiber protein 2
Cenexin; FLJ44866; MGC111096; MGC9034; ODF2/1; ODF2/2; ODF8484-kD; outer dense fiber of sperm tails 2; outer dense fiber protein 2; sperm tail structural protein ODF2 CT134/1, ODF2/2, ODF84, ODF2 outer dense fiber of sperm tails 2 outer dense fiber protein 2|cancer/testis antigen 134|cenexin 1|outer dense fiber of sperm tails, 84-kD|sperm tail structural protein|testis tissue sperm-binding protein Li 51e*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on ODF2, check out the ODF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ODF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
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