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- Table of Contents
CRCP antibody (M04247) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Western blot analysis of CRCP expression in HeLa cell lysate.
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Anti-CRCP Rabbit Monoclonal Antibody
CRCP antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IP, IF, IHC, ICC, WB |
Tested Applications
CRCP antibody was positively detected by WB in:
HeLa cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
ICC
IF
IP
Human
Recommend
Recommend
Recommend
Recommend
Mouse
Recommend
Recommend
Recommend
Recommend
Rat
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-CRCP Rabbit Monoclonal Antibody
View all CRCP Antibodies
View all CRCP Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
30C57
Isotype
IgG
Immunogen
A synthesized peptide derived from human CRCP
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
22 kDa
Calculated Molecular Weight
16.9 kDa
Cite This Product
Anti-CRCP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04247)
Protocols
Western Blot protocol for CRCP antibody
Publications
Application:
Species:
Artesunate alleviates post-central stroke pain by inhibiting metabotropic glutamate receptor 5 in the cerebral cortex in rats
Published: 2026-03-13
Journal: NEUROSCIENCE
Application: IF,WB
Impact Factor: 2.80
Species: Rat
PMID: 41833631
Review
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1 Review For Anti-CRCP Rabbit Monoclonal Antibody
This antibody is highly specific and efficient, suitable for Western blot detection of CRCP protein in MCF-7 cells, with only minor nonspecific bands observed.
| SKU | M04247 |
|---|---|
| Application | Western Blot |
| Sample | Human mcf-7 cells |
| Sample Processing Description | RIPA lysis buffer supplemented with a protease inhibitor cocktail was added to the cell culture dish, and the cells were lysed on ice for 30 min. The lysates were then centrifuged, and the supernatants were collected. Protein concentration was determined, and samples were adjusted to equal concentrations. Subsequently, 5× protein loading buffer was added, and the samples were denatured at 100 °C for 10 min. A total of 15 μL of protein sample was loaded per lane for SDS-PAGE electrophoresis. |
| Other Reagents | Blocking buffer |
| Primary Antibody | CRCP Rabbit Monoclonal Antibody |
| Primary Incubation | 1:500, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) |
| Secondary Incubation | 1:2000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | As shown in the figure, the expression levels of CRCP protein in MCF-7 cells at different drug treatment time points are presented. The Western blot results obtained with this antibody are clear; although there are a few faint nonspecific bands above, they do not affect result interpretation. It can be observed that the expression of the target protein in MCF-7 cells increases with longer drug treatment times. |
Siyuan Bu, LUTCM
Verified customer
Submitted 2025-12-26
Product Promise
We promise Anti-CRCP Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M04247
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026