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Product Info Summary
| SKU: | A05491 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Chemokine Receptor D6 ACKR2 Antibody
SKU/Catalog Number
A05491
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Chemokine Receptor D6 ACKR2 Antibody catalog # A05491. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Chemokine Receptor D6 ACKR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05491)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CCBP2. AA range:335-384
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05491 is reactive to ACKR2 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
43.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05491 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Immunofluorescence analysis of COS7 cells, using CCBP2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of various cells using Chemokine Receptor D6 Polyclonal Antibody
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Western blot analysis of lysates from HepG2 cells, using CCBP2 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HT-29 cells using CCBP2 antibody.
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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-Chemokine Receptor D6 ACKR2 Antibody (A05491)
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Customer Reviews
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5 Customer Q&As for Anti-Chemokine Receptor D6 ACKR2 Antibody
Question
We want to test anti-Chemokine Receptor D6 antibody A05491 on human lung for research purposes, then I may be interested in using anti-Chemokine Receptor D6 antibody A05491 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
The products we sell, including anti-Chemokine Receptor D6 antibody A05491, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-05
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-Chemokine Receptor D6 antibody A05491. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-17
Question
Would A05491 anti-Chemokine Receptor D6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-07-25
Answer
It shows on the product datasheet, A05491 anti-Chemokine Receptor D6 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-25
Question
Do you have a BSA free version of anti-Chemokine Receptor D6 antibody A05491 available?
Verified Customer
Verified customer
Asked: 2017-09-07
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Chemokine Receptor D6 antibody A05491 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-09-07
Question
I was wanting to use your anti-Chemokine Receptor D6 antibody for WB for human lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lung identification?
Z. Wu
Verified customer
Asked: 2016-02-23
Answer
It shows on the product datasheet, A05491 anti-Chemokine Receptor D6 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-02-23
