Anti-D6/ACKR2 Antibody Picoband™

Product Name

Anti-D6/ACKR2 Antibody Picoband™

View all CC Chemokine Receptor D6 Antibodies

SKU/Catalog Number

A05491-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-D6/ACKR2 Antibody Picoband™ catalog # A05491-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-D6/ACKR2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05491-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human D6/ACKR2 recombinant protein (Position: M138-A384).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05491-2 is reactive to ACKR2 in Human

Applications

A05491-2 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

43.443kDa

Background of CC Chemokine Receptor D6

This gene encodes a beta chemokine receptor, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptor-mediated signal transduction are critical for the recruitment of effector immune cells to the inflammation site. This gene is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members; however, its specific function remains unknown. This gene is mapped to chromosome 3p21.3, a region that includes a cluster of chemokine receptor genes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ACKR2 using anti-ACKR2 antibody (A05491-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human SW620 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACKR2 antigen affinity purified polyclonal antibody (Catalog # A05491-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACKR2 at approximately 55KD. The expected band size for ACKR2 is at 43KD.

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Figure 2. IHC analysis of ACKR2 using anti-ACKR2 antibody (A05491-2).
ACKR2 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACKR2 Antibody (A05491-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For ACKR2 (Source: Uniprot.org, NCBI)

Alternative Names

ACKR2; C-C chemokine receptor D6; CCBP2; CCR10CCR9D6CC-chemokine-binding receptor JAB61; chemokine (C-C) receptor 9; chemokine binding protein 2; Chemokine receptor CCR-10; Chemokine receptor CCR-9; chemokine receptor D6; chemokine-binding protein 2; Chemokine-binding protein D6; CMKBR9; CMKBR9chemokine (C-C motif) receptor 9; D6; hD6; MGC126678; MGC138250 ACKR2 CCBP2, CCR10, CCR9, CMKBR9, D6, hD6 atypical chemokine receptor 2 atypical chemokine receptor 2|C-C chemokine receptor D6|CC-chemokine-binding receptor JAB61|chemokine (C-C motif) receptor 9|chemokine (C-C) receptor 9|chemokine receptor CCR-10|chemokine receptor CCR-9|chemokine receptor D6|chemokine-binding protein 2|chemokine-binding protein D6

For more info on ACKR2, check out the ACKR2 Infographic

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In this infographic, you will see the following information for ACKR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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