Anti-CIITA Antibody Picoband™

CIITA antibody

Boster Bio Anti-CIITA Antibody Picoband™ catalog # PB9996. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PB9996
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-CIITA Antibody Picoband™

View all CIITA Antibodies

SKU/Catalog Number

PB9996

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CIITA Antibody Picoband™ catalog # PB9996. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CIITA Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9996)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CIITA recombinant protein (Position: E945-R1130). Human CIITA shares 84.4% amino acid (aa) sequence identity with mouse CIITA.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9996 is reactive to CIITA in Human, Mouse, Rat

Applications

PB9996 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

123 kDa

Calculated molecular weight

123.514kDa

Background of CIITA

CIITA is a human gene which is mapped to 16p13. This gene encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the "master control factor" for the expression of these genes. Also, the protein binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction. Several transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For CIITA (Source: Uniprot.org, NCBI)

Gene Name

CIITA

Full Name

MHC class II transactivator

Weight

123.514kDa

Alternative Names

C2TA; C2TAMHC class II transactivator type III; CIITAIV; class II, major histocompatibility complex, transactivator; MHC class II transactivator; MHC2TANLR family, acid domain containing; NLRA; nucleotide-binding oligomerization domain, leucine rich repeat and acid domaincontaining CIITA C2TAIV, MHC2TA, NLRA, CIITA class II major histocompatibility complex transactivator MHC class II transactivator|MHC class II transactivator type I|MHC class II transactivator type III|NLR family, acid domain containing|nucleotide-binding oligomerization domain, leucine rich repeat and acid domain containing

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CIITA, check out the CIITA Infographic

CIITA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CIITA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9996 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Autoregulatory loop between TGF-?1/miR-411-5p/SPRY4 and MAPK pathway in rhabdomyosarcoma modulates proliferation and differentiation

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4 Customer Q&As for Anti-CIITA Antibody Picoband™

Question

Does PB9996 anti-CIITA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

As indicated on the product datasheet, PB9996 anti-CIITA antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-25

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tendon using anti-CIITA antibody PB9996. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-18

Question

We are currently using anti-CIITA antibody PB9996 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?

S. Gonzalez

Verified customer

Asked: 2019-06-05

Answer

The anti-CIITA antibody (PB9996) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-05

Question

I see that the anti-CIITA antibody PB9996 works with IHC, what is the protocol used to produce the result images on the product page?

D. Johnson

Verified customer

Asked: 2015-05-18

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-05-18

Order DetailsPrice
PB9996

100μg

$370
PB9996-10ug

10μg sample (liquid)

$99
PB9996-Biotin

100 μg Biotin conjugated

$570
PB9996-Cy3

100 μg Cy3 conjugated

$570
PB9996-Dylight488

100 μg Dylight488 conjugated

$570
PB9996-Dylight550

100 μg Dylight550 conjugated

$570
PB9996-Dylight594

100 μg Dylight594 conjugated

$570
PB9996-FITC

100 μg FITC conjugated

$570
PB9996-HRP

100 μg HRP conjugated

$570
PB9996-APC

100 μg APC conjugated

$670
PB9996-PE

100 μg PE conjugated

$670
PB9996-carrier-free

Carrier Free

$370

More conjugates/formats

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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