Anti-Caspase 4/CASP4 Antibody
Rabbit IgG polyclonal antibody for Caspase-4(CASP4) detection. Tested with WB, IHC-P in Human. Cited in 2 publication(s).
Anti-Caspase 4/CASP4 Antibody Info At A Glance
Steroid receptor RNA activator 1 (SRA1) occurs as both an RNA transcript and as constantly expressed proteins. In addition, SRA1 mediates transcriptional coactivation of steroid receptors ligand-dependently via the steroid-binding domain (AF-2). SRA1 augments cellular proliferation and differentiation and stimulates apoptosis in vivo. SRA1 has a role in tumorigenesis. SRA1 participates in some activities such as: metabolism, adipogenesis and chromatin organization.
|Product Name||Anti-Caspase 4/CASP4 Antibody
See all CASP4 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for CASPASE 4/CASP4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CASPASE 4/CASP4 information: Molecular Weight: 43262 MW; Subcellular Localization: Endoplasmic reticulum membrane. Mitochondrion; Tissue Specificity: Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain.|
|Cite This Product||Anti-Caspase 4/CASP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1490)|
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|Anti-Caspase 4/CASP4 Antibody may replace the following items: sc 1227|sc 1227 R|sc 1229|sc 22173|sc 22173 R|sc 22174|sc 28228|sc 28229|sc 56056.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase 4(104-124aa DALKLCPHEEFLRLCKERAEE).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For CASP4 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||837|
|Species Of This Entry||Human|
|Superfamily||peptidase C14A family|
|Alternative Names||CASP4|apoptotic cysteine protease Mih1/TX; CASP4; CASP-4; caspase 4, apoptosis-related cysteine peptidase; caspase 4, apoptosis-related cysteine protease; Caspase4; Caspase-4; EC 3.4.22; EC 184.108.40.206; ICE(rel)II; ICE(rel)-II; ICEREL-II; ICH2; ICH-2; Mih1/TX; Protease ICH-2; Protease TX; TX|
|Post Tranlational Modifications||Cleavage; Phosphorylation; Glycosylation; Dephosphorylation; Deglycosylation; Ubiquitination|
|Subcellular Localization||Cytoplasm, cytosol. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion. Inflammasome. Secreted. Predominantly localizes to the endoplasmic reticulum (ER). Association with the ER membrane requires TMEM214 (PubMed:15123740). Released in the extracellular milieu by keratinocytes following UVB irradiation (PubMed:22246630).|
|Gene Location||11q22.3, on Chromasome 11, gene sequence: NC_000011.10 (104942866..104968596, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CASP4 (Source: Uniprot.org, NCBI)
|Protein Function||Inflammatory caspase (PubMed:7797510, PubMed:23516580, PubMed:25119034). Essential effector of NLRP3 inflammasome- dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS (PubMed:22246630, PubMed:26174085, PubMed:26173988, PubMed:26508369, PubMed:25964352). Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, and IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators (PubMed:24879791, PubMed:25964352). Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection (PubMed:25121752). In later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which ultimately results in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation. Involved in LPS-induced IL6 secretion; this activity may not require caspase enzymatic activity (PubMed:26508369). Involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP peptides found Alzheimer's patient brains (PubMed:15123740, PubMed:22246630, PubMed:23661706). Activated by direct binding to LPS without the need of an upstream sensor (PubMed:25119034). Does not directly process IL1B (PubMed:7743998, PubMed:7797592, PubMed:7797510). During non- canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation (PubMed:28314590).|
|Tissue Specificity||Widely expressed, including in keratinocytes and colonic and small intestinal epithelial cells (at protein level). Not detected in brain.|
|Research Areas||Apoptosis; Cancer; Caspases|
|Related Diseases||Malignant Neoplasms; Neoplasms; Endoplasmic Reticulum Stress; Carcinoma; Physiological Stress; Inflammation; Malignant Tumor Of Colon; Blind Vision; Leukemia; Malignant Paraganglionic Neoplasm; Alzheimer's Disease; Cell Transformation, Neoplastic; Melanoma; Malignant Neoplasm Of Breast; Mammary Neoplasms; Malignant Squamous Cell Neoplasm; Ovarian Neoplasm; Malignant Neoplasm Of Lung; Malignant Neoplasm Of Ovary; Neuroblastoma|
|Related Pathways||Cell Death; Pathogenesis; Induction Of Apoptosis; Translation; Cell Cycle; Cell Proliferation; Programmed Cell Death; Immune Response; Proteolysis; Inflammatory Response; Sensitization; Protein Folding; Rna Interference; Localization; Drug Resistance; Antigen Processing And Presentation; Cell Growth; Angiogenesis; Autophagy; Secretion|
|Background||Caspase 4 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue, and belongs to a family of cysteine proteases called caspases. The Caspase 4 gene is mapped to a P1 clone containing the ICE gene, which is located at chromosome 11q22.2-q22.3. It contains 8 coding exons.The function of caspase 4 is not fully known, but it is believed to be an inflammatory caspase, along with caspase 1, caspase 5(and the murine homolog caspase 11), with a role in the immune system.|
|Scientific References||PMID: 7743998 by Faucheu C., et al. A novel human protease similar to the interleukin-1 beta converting enzyme induces apoptosis in transfected cells.|
PMID: 7797592 by Munday N.A., et al. Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases.
Our Boster Quality Guarantee for Anti-Caspase 4/CASP4 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti Caspase 4 (PA1490) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: CEM Whole Cell Lysate at 40ug
Lane 5: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 43KD
Observed bind size: 20KD
IHC(P): Human Mammary Cancer Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 2
|Chen J, Wei H, Xie B, Wang B, Cheng J, Cheng J. Leuk Res. 2012 Dec;36(12):1526-35. Doi: 10.1016/J.Leukres.2012.08.018. Epub 2012 Sep 7. Endoplasmic Reticulum Stress Contributes To Arsenic Trioxide-Induced Apoptosis In Drug-Sensitive And -Resistant...||PubMed ID 22959511|
|Ji J, Yan X, Li Z, Lai Z, Liu J. Biomed Pharmacother. 2015 Feb;69:311-6. Doi: 10.1016/J.Biopha.2014.12.020. Epub 2014 Dec 19. Therapeutic Effects Of Intrathecal Versus Intravenous Monosialoganglioside Against Bupivacaine-Induced Spinal Neurotoxici...||PubMed ID 25661376|
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Note: sample size takes longer to process, average 5-7 days.
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