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- Table of Contents
Casp4 antibody (A02941-2) performs well in ELISA, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of A431 cells using anti-Caspase 4 antibody (A02941-2).
Overlay histogram showing A431 cells stained with A02941-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase 4 Antibody (A02941-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing A431 cells stained with A02941-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase 4 Antibody (A02941-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Designing a Western blot for CASP4? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the CASP4 Western Blot GuideBundle Save
Anti-Caspase 4/Casp4 Antibody Picoband®
Casp4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, ICC, WB |
Tested Applications
Casp4 antibody was positively detected by WB in:
mouse liver tissue, mouse testis tissue, mouse thymus tissue, mouse lung tissue, mouse HEPA1-6 whole cell, mouse NIH3T3 whole cell, mouse SP20 whole cell, rat liver tissue, rat testis tissue, rat stomach tissue, rat thymus tissue, human COLO-320 whole cell, human HepG2 whole cell, human 22RV1 whole cell, human PANC-1 whole cell, human SGC-7901 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Casp4 antibody was positively detected by ICC/IF in:
A431 cell
Casp4 antibody was positively detected by FC in:
A431 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence
5μg/ml
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
ELISA
Mouse
Tested
Recommend
Rat
Tested
Recommend
Human
Tested
Recommend
Product Information
Product Name
Anti-Caspase 4/Casp4 Antibody Picoband®
View all CASP4 Antibodies
View all CASP4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Caspase 4 recombinant protein (Position: E108-T195).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
43 kDa
Calculated Molecular Weight
42.7 kDa
Background of Casp4
Caspase 4 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue, and belongs to a family of cysteine proteases called caspases. The Caspase 4 gene is mapped to a P1 clone containing the ICE gene, which is located at chromosome 11q22.2-q22.3. It contains 8 coding exons. The function of caspase 4 is not fully known, but it is believed to be an inflammatory caspase, along with caspase 1, caspase 5 (and the murine homolog caspase 11), with a role in the immune system.
Cite This Product
Anti-Caspase 4/Casp4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02941-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
94%
High Homology
Rabbit
65%
Low homology
Monkey
65%
Low homology
Human
64%
Low homology
Sheep
63%
Low homology
Dog
61%
Low homology
Zebrafish
39%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Casp4 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for Casp4 antibody
Flow Cytometry (FC) protocol for Casp4 antibody
Publications
Application:
Species:
Endoplasmic reticulum stress contributes to arsenic trioxide-induced apoptosis in drug-sensitive and -resistant leukemia cells
Published: 2012-09-06
Journal: LEUKEMIA RESEARCH
Application: WB
Impact Factor: 2.92
Species: Human
PMID: 22959511
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Product Promise
We promise Anti-Caspase 4/Casp4 Antibody Picoband® performs excellently in relevant applications for ELISA, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A02941-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Nov 1, 2026