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Product Info Summary
| SKU: | M01357-3 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CK18 (Cytokeratin-18) KRT18 Rabbit Monoclonal Antibody, Clone#RM279
SKU/Catalog Number
M01357-3
Size
100uL
Form
Liquid
Description
Boster Bio Anti-CK18 (Cytokeratin-18) KRT18 Rabbit Monoclonal Antibody, Clone#RM279 (Catalog # M01357-3). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CK18 (Cytokeratin-18) KRT18 Rabbit Monoclonal Antibody, Clone#RM279 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01357-3)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM279
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the N-terminus of human Cytokeratin-18.
Cross-reactivity
This antibody reacts to human CK18 (Cytokeratin-18).
Reactive Species
M01357-3 is reactive to KRT18 in Human
Calculated molecular weight
48.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01357-3 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:1000-1:4000 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of HeLa cells lysates using Anti-CK18 Rabbit Monoclonal Antibody (Clone RM279) at a 1:1000 dilution.
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-CK18 Rabbit Monoclonal Antibody (Clone RM279) at a 1:4000 dilution.
Specific Publications For Anti-CK18 (Cytokeratin-18) KRT18 Rabbit Monoclonal Antibody, Clone#RM279 (M01357-3)
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Customer Q&As
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16 Customer Q&As for Anti-CK18 (Cytokeratin-18) KRT18 Rabbit Monoclonal Antibody, Clone#RM279
Question
Is a blocking peptide available for product anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 (M01357-3)?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
We do provide the blocking peptide for product anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 (M01357-3). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-04-30
Question
Does anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 M01357-3 work for IHC with colon?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
According to the expression profile of colon, KRT18 is highly expressed in colon. So, it is likely that anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 M01357-3 will work for IHC with colon.
Boster Scientific Support
Answered: 2020-04-27
Question
I have attached the WB image, lot number and protocol we used for colon using anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 M01357-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-24
Question
Is this M01357-3 anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 reactive to the isotypes of KRT18?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
The immunogen of M01357-3 anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 is A peptide corresponding to the N-terminus of human Cytokeratin-18. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-19
Question
I see that the anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 M01357-3 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-01-30
Question
I have a question about product M01357-3, anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01357-3 anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-12
Question
I am looking for to test anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 M01357-3 on human colon for research purposes, then I may be interested in using anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 M01357-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
The products we sell, including anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 M01357-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-20
Question
Is there a BSA free version of anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 M01357-3 available?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 M01357-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-20
Question
Does M01357-3 anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-03-01
Answer
It shows on the product datasheet, M01357-3 anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-03-01
Question
you antibody using your anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 for intermediate filament cytoskeleton organization studies. Has this antibody been tested with western blotting on breast cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-12-05
Answer
Thank you for your inquiry. This M01357-3 anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 is validated on hela cells, breast cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-12-05
Question
My team were well pleased with the WB result of your anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2018-06-28
Answer
From what I have seen in literature, placenta uterus does express KRT18. Generally KRT18 expresses in cytoplasm, perinuclear region. nucleus,. Regarding which tissues have KRT18 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 17924679, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Colon, Pancreas, Placenta, and Uterus, Pubmed ID: 15489334
Colon carcinoma, Pubmed ID: 9150948, 24129315
Liver, Pubmed ID: 2422083, 24275569
Placenta, Pubmed ID: 2434380
Vulva, Pubmed ID: 2434381
Boster Scientific Support
Answered: 2018-06-28
Question
I was wanting to use your anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 for IHC for human colon on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human colon identification?
Verified Customer
Verified customer
Asked: 2018-02-13
Answer
You can see on the product datasheet, M01357-3 anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human colon in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-13
Question
We ordered your anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2018-01-03
Answer
I looked at the website and datasheets of our anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2018-01-03
Question
We have observed staining in human cervix carcinoma. Do you have any suggestions? Is anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 supposed to stain cervix carcinoma positively?
H. Johnson
Verified customer
Asked: 2017-11-13
Answer
Based on literature cervix carcinoma does express KRT18. Based on Uniprot.org, KRT18 is expressed in adenohypophysis, placenta, cervix, colon, pancreas, placenta uterus, vulva, liver, colon carcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have KRT18 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 17924679, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Colon, Pancreas, Placenta, and Uterus, Pubmed ID: 15489334
Colon carcinoma, Pubmed ID: 9150948, 24129315
Liver, Pubmed ID: 2422083, 24275569
Placenta, Pubmed ID: 2434380
Vulva, Pubmed ID: 2434381
Boster Scientific Support
Answered: 2017-11-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon using anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 M01357-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-31
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-31
Question
We are currently using anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 M01357-3 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
J. Mangal
Verified customer
Asked: 2015-05-26
Answer
The anti-CK18 (Cytokeratin-18) Rabbit Monoclonal antibody, Clone#RM279 (M01357-3) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-05-26
