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Product Info Summary
| SKU: | A03073 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-PID MTA2 Antibody
SKU/Catalog Number
A03073
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PID MTA2 Antibody (Catalog # A03073). Tested in ELISA, WB, ICC, IF, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PID antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PID MTA2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03073)
Host
Rabbit
Contents
PID Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PID antibody was raised against a 17 amino acid peptide near the carboxy terminus of human PID. The immunogen is located within the last 50 amino acids of PID.
Cross-reactivity
PID antibody is predicted to not cross-react with MTA2
Reactive Species
A03073 is reactive to MTA2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
75.0 kDa
Background of MTA2
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 pathway, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The transcriptional activity of p53 is modulated by protein stability and acetylation. PID/MTA2, also termed MTA1-L1, was found to be a subunit of nucleosome remodeling and deacetylating (NRD/NuRD) complex. PID/MTA2 modulates the enzymatic activity of the histone deacetylase complex and its expression reduces the levels of acetylated p53. Deacetylation of p53 by PID/MTA2 represses p53-dependent transcriptional activation and modulates p53-mediated cell growth arrest and apoptosis. PID/MTA2 is ubiquitously expressed in human tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03073 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PID antibody can be used for detection of PID by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and Immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PID expression in HeLa whole cell lysates in the absence (A) or presence (B) of blocking peptide with PID antibody at 1 μg/mL.
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Immunocytochemistry staining of HeLa using PID antibody at 10 μg/mL.
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Immunofluorescence of PID in HeLa cells with PID antibody at 10 ug/mL.
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Immunohistochemistry of PID in human small intestine tissue with PID antibody at 2.5 μg/ml.
Specific Publications For Anti-PID MTA2 Antibody (A03073)
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