Anti-Claudin 2 CLDN2 Antibody

Boster Bio Anti-Claudin 2 CLDN2 Antibody catalog # A03033. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A03033
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Claudin 2 CLDN2 Antibody

See all CLDN2 products

SKU/Catalog Number

A03033

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Claudin 2 CLDN2 Antibody catalog # A03033. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Claudin 2 CLDN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03033)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

Synthesized peptide derived from human Claudin 2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03033 is reactive to CLDN2 in Human, Mouse, Rat

Applications

A03033 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:3000
IHC, 1:50-1:100

Validation Images & Assay Conditions

Gene/Protein Information For CLDN2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P57739

Gene ID

9075

Gene Name

CLDN2

Full Name

Claudin-2

Weight

24.549kDa

Superfamily

claudin family

Alternative Names

Claudin-2 CLDN2 claudin 2 claudin-2|SP82

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CLDN2, check out the CLDN2 Infographic

CLDN2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CLDN2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A03033

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Find answers in Q&As, reviews.

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16 Customer Q&As for Anti-Claudin 2 CLDN2 Antibody

Question

We need using your anti-Claudin 2 antibody for calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules studies. Has this antibody been tested with western blotting on huvec cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-09-18

Answer

Thank you for your inquiry. This A03033 anti-Claudin 2 antibody is validated on huvec cells, breast carcinoma tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-09-18

Question

I was wanting to use your anti-Claudin 2 antibody for WB for mouse thalamus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse thalamus identification?

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

You can see on the product datasheet, A03033 anti-Claudin 2 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse thalamus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-11

Question

Is this A03033 anti-Claudin 2 antibody reactive to the isotypes of CLDN2?

Verified Customer

Verified customer

Asked: 2019-06-25

Answer

The immunogen of A03033 anti-Claudin 2 antibody is Synthesized peptide derived from human Claudin 2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-25

Question

Our team were well pleased with the WB result of your anti-Claudin 2 antibody. However we have seen positive staining in teratocarcinoma cell junction using this antibody. Is that expected? Could you tell me where is CLDN2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-03-22

Answer

From what I have seen in literature, teratocarcinoma does express CLDN2. Generally CLDN2 expresses in cell junction, tight junction. Regarding which tissues have CLDN2 expression, here are a few articles citing expression in various tissues:
Colon epithelium, Pubmed ID: 11934881
Colon, and Skin, Pubmed ID: 15489334
Teratocarcinoma, Pubmed ID: 16303743
Thalamus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-03-22

Question

Does A03033 anti-Claudin 2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-02-18

Answer

It shows on the product datasheet, A03033 anti-Claudin 2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-02-18

Question

Can you help my question with product A03033, anti-Claudin 2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

J. Miller

Verified customer

Asked: 2019-01-08

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03033 anti-Claudin 2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-01-08

Question

We are currently using anti-Claudin 2 antibody A03033 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

B. Krishna

Verified customer

Asked: 2018-05-15

Answer

The anti-Claudin 2 antibody (A03033) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-05-15

Question

We have been able to see staining in mouse colon skin. Are there any suggestions? Is anti-Claudin 2 antibody supposed to stain colon skin positively?

Verified Customer

Verified customer

Asked: 2018-05-08

Answer

From literature colon skin does express CLDN2. From Uniprot.org, CLDN2 is expressed in kidney epithelium, colon epithelium, thalamus, teratocarcinoma, colon skin, among other tissues. Regarding which tissues have CLDN2 expression, here are a few articles citing expression in various tissues:
Colon epithelium, Pubmed ID: 11934881
Colon, and Skin, Pubmed ID: 15489334
Teratocarcinoma, Pubmed ID: 16303743
Thalamus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-05-08

Question

Is a blocking peptide available for product anti-Claudin 2 antibody (A03033)?

H. Li

Verified customer

Asked: 2017-12-04

Answer

We do provide the blocking peptide for product anti-Claudin 2 antibody (A03033). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-12-04

Question

My lab would like to test anti-Claudin 2 antibody A03033 on mouse thalamus for research purposes, then I may be interested in using anti-Claudin 2 antibody A03033 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-10-24

Answer

The products we sell, including anti-Claudin 2 antibody A03033, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-10-24

Question

We bought anti-Claudin 2 antibody for IHC on colon skin a few years ago. I am using rat, and We want to use the antibody for WB next. My question regards examining colon skin as well as thalamus in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2017-10-12

Answer

I looked at the website and datasheets of our anti-Claudin 2 antibody and I see that A03033 has been validated on rat in both IHC and WB. Thus A03033 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-10-12

Question

I see that the anti-Claudin 2 antibody A03033 works with WB, what is the protocol used to produce the result images on the product page?

R. Williams

Verified customer

Asked: 2017-09-26

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-09-26

Question

Do you have a BSA free version of anti-Claudin 2 antibody A03033 available?

Verified Customer

Verified customer

Asked: 2017-09-05

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Claudin 2 antibody A03033 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-09-05

Question

See below the WB image, lot number and protocol we used for thalamus using anti-Claudin 2 antibody A03033. Please let me know if you require anything else.

P. Gonzalez

Verified customer

Asked: 2016-01-20

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-01-20

Question

Will anti-Claudin 2 antibody A03033 work for WB with thalamus?

J. Moore

Verified customer

Asked: 2014-05-07

Answer

According to the expression profile of thalamus, CLDN2 is highly expressed in thalamus. So, it is likely that anti-Claudin 2 antibody A03033 will work for WB with thalamus.

Boster Scientific Support

Answered: 2014-05-07

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thalamus using anti-Claudin 2 antibody A03033. Let me know if you need anything else.

K. Huang

Verified customer

Asked: 2013-06-10

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-06-10

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A03033
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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