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Product Info Summary
| SKU: | M00122-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00122-1
BM3941 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody catalog # M00122-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00122-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HI-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cleaved PARP
Reactive Species
M00122-1 is reactive to PARP1 in Human, Mouse, Rat
Observed Molecular Weight
25 kDa
Calculated molecular weight
113.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00122-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
WB: human 293T whole cell, human THP-1 whole cell, human SH-SY5Y whole cell, human K562 whole cell, mouse Raw2647 whole cell, mouse NIH/3T3 whole cell
IHC: human kidney tissue
Validation Images & Assay Conditions
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Western blot analysis of PARP1 using anti-PARP1 antibody (M00122-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: mouse Raw264.7 whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP1 antigen affinity purified polyclonal antibody (Catalog # M00122-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP1 at approximately 25 kDa. The expected band size for PARP1 is at 25,113 kDa.
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Immunohistochemical analysis of paraffin-embedded human kidney, using Cleaved PARP Antibody.
Specific Publications For Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody (M00122-1)
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Customer Q&As
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4 Customer Q&As for Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody
Question
We bought anti-Cleaved PARP Rabbit Monoclonal antibody for IHC on liver in the past. I am using rat, and We are going to use the antibody for WB next. I am looking for examining liver as well as leukemic t-cell in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
I looked at the website and datasheets of our anti-Cleaved PARP Rabbit Monoclonal antibody and it seems that M00122-1 has been tested on rat in both IHC and WB. Thus M00122-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-16
Question
Our team were happy with the WB result of your anti-Cleaved PARP Rabbit Monoclonal antibody. However we have seen positive staining in colon carcinoma ovarian carcinoma nucleus using this antibody. Is that expected? Could you tell me where is PARP1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
From what I have seen in literature, colon carcinoma ovarian carcinoma does express PARP1. Generally PARP1 expresses in nucleus. Regarding which tissues have PARP1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, and Ovarian carcinoma, Pubmed ID: 17177976
Embryonic kidney, Pubmed ID: 17525332
Fibroblast, Pubmed ID: 3120710, 2824474, 2891139
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-12-24
Question
We have been able to see staining in human cervix carcinoma erythroleukemia. What should we do? Is anti-Cleaved PARP Rabbit Monoclonal antibody supposed to stain cervix carcinoma erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
Based on literature cervix carcinoma erythroleukemia does express PARP1. Based on Uniprot.org, PARP1 is expressed in heart left ventricle, fibroblast, brain, colon carcinoma ovarian carcinoma, embryonic kidney, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have PARP1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, and Ovarian carcinoma, Pubmed ID: 17177976
Embryonic kidney, Pubmed ID: 17525332
Fibroblast, Pubmed ID: 3120710, 2824474, 2891139
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-12-19
Question
We are currently using anti-Cleaved PARP Rabbit Monoclonal antibody M00122-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
H. Zhang
Verified customer
Asked: 2015-04-06
Answer
The anti-Cleaved PARP Rabbit Monoclonal antibody (M00122-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-06
