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- Table of Contents
PARP1 antibody (M00122-1) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 11 Publications
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Immunohistochemical analysis of paraffin-embedded human kidney, using Cleaved PARP Antibody.
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Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody
PARP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
PARP1 antibody was positively detected by WB in:
human 293T whole cell, human THP-1 whole cell, human SH-SY5Y whole cell, human K562 whole cell, mouse Raw2647 whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PARP1 antibody was positively detected by IHC in:
human kidney tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody
View all PARP1 Antibodies
View all PARP1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HI-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cleaved PARP
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
25 kDa
Calculated Molecular Weight
113.1 kDa
Cite This Product
Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00122-1)
Protocols
Western Blot protocol for PARP1 antibody
Immunohistochemistry (IHC) protocol for PARP1 antibody
Publications
Application:
Species:
Virtual screening and MD simulation-guided discovery of CDK12-IN-3, a potent ALK inhibitor suppressing neuroblastoma growth in vitro and in vivo
Published: 2026-08-10
Journal: Frontiers in Chemistry
Application: WB
Impact Factor: 5.30
Species: Human,Mouse
PMID: 42683163
Virtual Screening and MD Simulation-Driven Discovery of USP7 Inhibitors BML-284
Published: 2026-02-19
Journal: ACS Omega
Application: WB
Impact Factor: 4.30
Species: Human
PMID: 41799095
α-hederin inhibits cervical cancer progression by inducing DNA damage-dependent cell cycle blockade and apoptosis
Published: 2025-10-03
Journal: BMC CANCER
Application: WB
Impact Factor: 3.40
Species: Human
PMID: 41044707
Smart biodegradable hydrogels incorporating a photoactive Ir(III) complex for NIR-triggered oral cancer treatment
Published: 2025-07-14
Journal: Cancer Nanotechnology
Application: WB
Impact Factor: 4.80
Species: Human
Thiazolidinedione-Based Structure Modification of Celastrol Provides Thiazolidinedione-Conjugated Derivatives as Potent Agents against Non-Small-Cell Lung Cancer Cells through a Mitochondria-Mediated Apoptotic Pathway
Published: 2022-03-08
Journal: JOURNAL OF NATURAL PRODUCTS
Application: WB
Impact Factor: 4.80
Species: Human
PMID: 35255689
Piperine Inhibits Cell Proliferation and Induces Apoptosis of Human Gastric Cancer Cells by Downregulating Phosphatidylinositol 3-Kinase (PI3K)/Akt Pathway
Published: 2020-12-31
Journal: MEDICAL SCIENCE MONITOR
Application: WB
Impact Factor: 1.92
Species: Human
PMID: 33382670
Circular RNA EIF6 (Hsa_circ_0060060) sponges miR-144-3p to promote the cisplatin-resistance of human thyroid carcinoma cells by autophagy regulation
Published: 2018-12-12
Journal: Aging-US
Application: WB
Impact Factor: 5.18
Species: Human
PMID: 30540564
Blockage of High-Affinity Choline Transporter Increases Visceral Hypersensitivity in Rats with Chronic Stress
Published: 2018-04-04
Journal: Gastroenterology Research and Practice
Application: WB
Impact Factor: 1.86
Species: Human
PMID: 29849603
Cell death-inducing DFF45-like effector C gene silencing alleviates pulmonary vascular remodeling in a type 2 diabetic rat model
Published: 2017-10-27
Journal: Journal of Diabetes Investigation
Application: WB
Impact Factor: 3.04
Species: Rat
PMID: 29078040
The natural secolignan peperomin E induces apoptosis of human gastric carcinoma cells via the mitochondrial and PI3K/Akt signaling pathways in vitro and in vivo
Published: 2016-05-11
Journal: PHYTOMEDICINE
Application: WB
Impact Factor: 2.94
Species: Human
PMID: 27288917
Neuroprotective Properties of Picroside II in a Rat Model of Focal Cerebral Ischemia
Published: 2010-11-16
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: IHC
Impact Factor: 1.39
Species: Rat
PMID: 21151457
Review
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Product Promise
We promise Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00122-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026