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- Table of Contents
PARP1 antibody (PB9309) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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Flow Cytometry analysis of A431 cells using anti-PARP antibody (PB9309).
Overlay histogram showing A431 cells stained with PB9309 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PARP Antibody (PB9309, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A431 cells stained with PB9309 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PARP Antibody (PB9309, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-PARP/PARP1 Antibody Picoband®
PARP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
PARP1 antibody was positively detected by WB in:
human Hela whole cell, human HepG2 whole cell, human COLO-320 whole cell, human Jurkat whole cell, rat PC-12 whole cell, mouse NIH3T3 whole cell, mouse HEPA1-6 whole cell, human Hela- WT whole cell, human Hela-GPX4 KO whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PARP1 antibody was positively detected by IHC in:
Mouse Intestine tissue, Rat Brain tissue, Human Placenta tissue, mouse liver, rat kidney, rat spleen, human tosil, mouse kidney, human bladder cancer, human stomach cancer, human pancreas cancer
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
PARP1 antibody was positively detected by ICC in:
A549 Cell
PARP1 antibody was positively detected by IF in:
human breast cancer tissue, human bladder cancer, human pancreas cancer, human stomach cancer
PARP1 antibody was positively detected by FC in:
A431 cells
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunocytochemistry
2μg/ml
Human, Rat
Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Tested
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-PARP/PARP1 Antibody Picoband®
View all PARP1 Antibodies
View all PARP1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PARP recombinant protein (Position: Q670-R858). Human PARP shares 94% and 95% amino acid (aa) sequence identity with mouse and rat PARP, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
120 kDa
Calculated Molecular Weight
113.1 kDa
Background of PARP1
Poly [ADP-ribose] polymerase 1 (PARP1), also known as ADPRT or PPOL is an enzyme that in humans is encoded by the PARP1 gene. PARP1 gene is mapped to 1q42.12. This gene encodes a chromatin-associated enzyme, poly (ADP-ribosyl)transferase, which modifies various nuclear proteins by poly (ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Cite This Product
Anti-PARP/PARP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9309)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Dog
98%
High Homology
Cow
96%
High Homology
Sheep
96%
High Homology
Mouse
95%
High Homology
Rabbit
95%
High Homology
Rat
95%
High Homology
Zebrafish
78%
Moderate Homology
Drosophila
48%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PARP1 antibody
Immunohistochemistry (IHC) protocol for PARP1 antibody
Immunocytochemistry (ICC) protocol for PARP1 antibody
Immunofluorescence (IF) protocol for PARP1 antibody
Flow Cytometry (FC) protocol for PARP1 antibody
Publications
Application:
Species:
Virtual screening and MD simulation-guided discovery of CDK12-IN-3, a potent ALK inhibitor suppressing neuroblastoma growth in vitro and in vivo
Published: 2026-08-10
Journal: Frontiers in Chemistry
Application: WB
Impact Factor: 5.30
Species: Human,Mouse
PMID: 42683163
MiR-27b-3p Reduces the Efficacy of Propranolol in the Treatment of Infantile Hemangioma by Inhibiting the Expression of Apaf-1
Published: 2025-04-30
Journal: Biomedicines
Application: WB
Impact Factor: 3.90
Species: Human
Discovery of Highly Potent and Orally Bioavailable Histone Deacetylase 3 Inhibitors as Immunomodulators and Enhancers of DNA-Damage Response in Cancer Therapy
Published: 2025-01-28
Journal: JOURNAL OF MEDICINAL CHEMISTRY
Application: WB
Impact Factor: 6.80
Species: Mouse
PMID: 39873221
Water Extract of Rice False Smut Balls Activates Nrf2/HO-1 and Apoptosis Pathways, Causing Liver Injury
Published: 2023-08-23
Journal: Rice Science
Application: WB
Impact Factor: 4.80
Species: Mouse
(2E)-1-(2,4,6-Trimethoxyphenyl)-3-(4-chlorophenyl)prop-2-en-1-one, a Chalcone Derivative, Promotes Apoptosis by Suppressing RAS-ERK and AKT/FOXO3a Pathways in Hepatocellular Carcinoma Cells
Published: 2023-06-14
Journal: CHEMISTRY & BIODIVERSITY
Application: WB
Impact Factor: 2.90
Species: Human
PMID: 37314937
CIDEC silencing attenuates diabetic nephropathy via inhibiting apoptosis and promoting autophagy
Published: 2021-03-02
Journal: Journal of Diabetes Investigation
Application: WB,IHC
Impact Factor: 4.23
Species: Rat
PMID: 33655702
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Product Promise
We promise Anti-PARP/PARP1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9309
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026