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Product Info Summary
| SKU: | PA1798 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-TFPI Antibody Picoband®
SKU/Catalog Number
PA1798
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TFPI Antibody catalog # PA1798. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TFPI Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1798)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human TFPI.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1798 is reactive to TFPI in Human
Observed Molecular Weight
55 kDa
Calculated molecular weight
35.0 kDa
Background of TFPI
TFPI (Tissue factor pathway inhibitor) also known as EPI, LACI, TFI and TFPT1 is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). While Xa is inhibited, the Xa-TFPI complex can subsequently also inhibit the FVIIa-tissue factor complex. TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM. The gene for TFPI is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI has a relative molecular mass of 34,000 to 40,000 depending on the degree of proteolysis of the C-terminal region.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1798 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: SMMC Whole Cell
IHC: Human Placenta Tissue, Human Lung Cancer Tissue
Validation Images & Assay Conditions
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Anti-TFPI antibody, PA1798, Western blotting
All lanes: Anti TFPI (PA1798) at 0.5ug/ml
WB: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 26KD
Observed bind size: 55KD
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Anti-TFPI antibody, PA1798, IHC(P)
IHC(P): Human Placenta Tissue
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Anti-TFPI antibody, PA1798, IHC(P)
IHC(P): Human Lung Cancer Tissue
Specific Publications For Anti-TFPI Antibody Picoband® (PA1798)
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Customer Q&As
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6 Customer Q&As for Anti-TFPI Antibody Picoband®
Question
I was wanting to use to test anti-TFPI antibody PA1798 on human placenta for research purposes, then I may be interested in using anti-TFPI antibody PA1798 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
The products we sell, including anti-TFPI antibody PA1798, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-10
Question
We are currently using anti-TFPI antibody PA1798 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
The anti-TFPI antibody (PA1798) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-20
Question
Is there a BSA free version of anti-TFPI antibody PA1798 available?
R. Rodriguez
Verified customer
Asked: 2019-05-16
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TFPI antibody PA1798 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-16
Question
Will anti-TFPI antibody PA1798 work for IHC with placenta?
F. Li
Verified customer
Asked: 2018-12-26
Answer
According to the expression profile of placenta, TFPI is highly expressed in placenta. So, it is likely that anti-TFPI antibody PA1798 will work for IHC with placenta.
Boster Scientific Support
Answered: 2018-12-26
Question
I have a question about product PA1798, anti-TFPI antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-09-07
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1798 anti-TFPI antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-07
Question
Is a blocking peptide available for product anti-TFPI antibody (PA1798)?
R. Kulkarni
Verified customer
Asked: 2014-07-28
Answer
We do provide the blocking peptide for product anti-TFPI antibody (PA1798). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2014-07-28
