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Product Info Summary
| SKU: | M00577 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CREB CREB1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00577
BM4288 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CREB CREB1 Rabbit Monoclonal Antibody catalog # M00577. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CREB CREB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00577)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DCO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CREB
Reactive Species
M00577 is reactive to CREB1 in Human, Mouse, Rat
Observed Molecular Weight
45 kDa
Calculated molecular weight
36.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00577 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: NIH/3T3 cell
IHC: mouse brain tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of CREB expression in NIH/3T3 cell lysate.
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Immunohistochemical analysis of paraffin-embedded mouse brain, using CREB Antibody.
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Immunofluorescent analysis of Hela cells, using CREB Antibody .
Specific Publications For Anti-CREB CREB1 Rabbit Monoclonal Antibody (M00577)
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-CREB CREB1 Rabbit Monoclonal Antibody
Question
We ordered your anti-CREB Rabbit Monoclonal antibody for Flow Cytometry on lung in a previous project. I am using human, and I plan to use the antibody for IF next. We want examining lung as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
I took a look at the website and datasheets of our anti-CREB Rabbit Monoclonal antibody and it seems that M00577 has been tested on human in both Flow Cytometry and IF. Thus M00577 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-27
Question
Does anti-CREB Rabbit Monoclonal antibody M00577 work for Flow Cytometry with eye?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
According to the expression profile of eye, CREB1 is highly expressed in eye. So, it is likely that anti-CREB Rabbit Monoclonal antibody M00577 will work for Flow Cytometry with eye.
Boster Scientific Support
Answered: 2019-11-08
Question
I see that the anti-CREB Rabbit Monoclonal antibody M00577 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-05
Question
Can you help my question with product M00577, anti-CREB Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00577 anti-CREB Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-30
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-CREB Rabbit Monoclonal antibody M00577. Let me know if you need anything else.
R. Johnson
Verified customer
Asked: 2019-10-14
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-14
Question
you antibody using your anti-CREB Rabbit Monoclonal antibody for negative regulation of neuron death studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
E. Banerjee
Verified customer
Asked: 2019-10-08
Answer
I appreciate your inquiry. This M00577 anti-CREB Rabbit Monoclonal antibody is tested on mouse brain. It is guaranteed to work for Flow Cytometry, IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-08
Question
Does anti-CREB Rabbit Monoclonal antibody M00577 work on goat IF with eye?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
Our lab technicians have not validated anti-CREB Rabbit Monoclonal antibody M00577 on goat. You can run a BLAST between goat and the immunogen sequence of anti-CREB Rabbit Monoclonal antibody M00577 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat eye in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-07
Question
Is this M00577 anti-CREB Rabbit Monoclonal antibody reactive to the isotypes of CREB1?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
The immunogen of M00577 anti-CREB Rabbit Monoclonal antibody is A synthesized peptide derived from human CREB. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-27
Question
Is there a BSA free version of anti-CREB Rabbit Monoclonal antibody M00577 available?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CREB Rabbit Monoclonal antibody M00577 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-26
Question
We are currently using anti-CREB Rabbit Monoclonal antibody M00577 for rat tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
The anti-CREB Rabbit Monoclonal antibody (M00577) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-16
Question
I was wanting to use your anti-CREB Rabbit Monoclonal antibody for Flow Cytometry for human eye on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human eye identification?
Verified Customer
Verified customer
Asked: 2019-04-11
Answer
It shows on the product datasheet, M00577 anti-CREB Rabbit Monoclonal antibody has been validated for Flow Cytometry, IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human eye in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-11
Question
Would M00577 anti-CREB Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Miller
Verified customer
Asked: 2019-02-20
Answer
It shows on the product datasheet, M00577 anti-CREB Rabbit Monoclonal antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-20
Question
Here is the WB image, lot number and protocol we used for eye using anti-CREB Rabbit Monoclonal antibody M00577. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-07-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-06
Question
We have been able to see staining in human lung. What should we do? Is anti-CREB Rabbit Monoclonal antibody supposed to stain lung positively?
K. Li
Verified customer
Asked: 2018-02-02
Answer
According to literature lung does express CREB1. According to Uniprot.org, CREB1 is expressed in lung, testis, eye, embryonic kidney, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have CREB1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 15579595
Boster Scientific Support
Answered: 2018-02-02
Question
We need to test anti-CREB Rabbit Monoclonal antibody M00577 on human eye for research purposes, then I may be interested in using anti-CREB Rabbit Monoclonal antibody M00577 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Collins
Verified customer
Asked: 2017-10-13
Answer
The products we sell, including anti-CREB Rabbit Monoclonal antibody M00577, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-13
Question
Is a blocking peptide available for product anti-CREB Rabbit Monoclonal antibody (M00577)?
Verified Customer
Verified customer
Asked: 2017-05-12
Answer
We do provide the blocking peptide for product anti-CREB Rabbit Monoclonal antibody (M00577). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-05-12
Question
Our team were content with the WB result of your anti-CREB Rabbit Monoclonal antibody. However we have been able to see positive staining in lung nucleus using this antibody. Is that expected? Could you tell me where is CREB1 supposed to be expressed?
E. Parker
Verified customer
Asked: 2014-09-15
Answer
From literature, lung does express CREB1. Generally CREB1 expresses in nucleus. Regarding which tissues have CREB1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 15579595
Boster Scientific Support
Answered: 2014-09-15
