Product Info Summary
SKU: | EZ0309 |
---|---|
Size: | For 5 plates, 96 wells each, reagents only, plates are not included |
Reactive Species: | Mouse |
Application: | ELISA |
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Product info
Product Name
Mouse BDNF ELISA Kit EZ-Set™ (DIY Antibody Pairs)
SKU/Catalog Number
EZ0309
Size
For 5 plates, 96 wells each, reagents only, plates are not included
*Question: How many samples can I assay/run in this kit?
Description
Mouse BDNF ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse BDNF in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate).
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse BDNF ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0309)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: sf21; Immunogen sequence: H129-R247
Assay Range
31.2 pg/ml - 2,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EZ0309 is reactive to BDNF in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EZ0309 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Catalog number | Description | Quantity | Storage of opened/reconstituted material |
---|---|---|---|
EZ0309-CA | Rat anti- mouse bdnf monoclonal antibody (Capture Antibody) | 500 µl, 0.4 mg/mL (recommended dilution 1:100) | Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. |
EZ0309-DA | Biotinylated goat anti- mouse bdnf polyclonal antibody (Detection Antibody) | 500 µl, 4.5 µg/mL (recommended dilution 1:100) | |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µl (recommended dilution 1:100) | |
EK0309-ST | Lyophilized recombinant mouse bdnf standard | 10 ng/tube×3 | Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Incubator.
- 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 5. Clean tubes and Eppendorf tubes.
- 6. 96 well microplate (Cat# AR1100)
- 7. Plate Sealers.
- 8. Capture Antibody Diluent: PBS.
- 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. - *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.018 | 0.046 | 0.059 | 0.103 | 0.219 | 0.451 | 0.979 | 1.863 |
Data Example Images
Click image to see more details
Mouse BDNF EZ Set ELISA Kit standard curve
Protein Target Info & Infographic
Gene/Protein Information For BDNF (Source: Uniprot.Org, NCBI)
Gene Name
BDNF
Full Name
Brain-derived neurotrophic factor
Weight
28123 MW
Superfamily
NGF-beta family
Alternative Names
Brain-derived neurotrophic factor;BDNF;Bdnf; BDNF ANON2, BULN2 brain derived neurotrophic factor brain-derived neurotrophic factor|abrineurin|neurotrophin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on BDNF, check out the BDNF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for BDNF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse BDNF ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0309)
Hello CJ!
EZ0309 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Neurotherapeutic Effect of Cord Blood Derived CD45 Hematopoietic Cells in Mice after Traumatic Brain Injury
Chronic Hippocampal Abnormalities and Blunted HPA Axis in an Animal Model of Repeated Unpredictable Stress
Ondansetron ameliorates depression associated with obesity in high-fat diet fed experimental mice: An investigation-based on the behavioral, biochemical, and molecular approach
Magnetic Beads Enhance Adhesion of NIH 3T3 Fibroblasts: A Proof-of-Principle In Vitro Study for Implant-Mediated Long-Term Drug Delivery to the Inner Ear
The effects of EGb761 on lipopolysaccharide-induced depressive-like behaviour in C57BL/6J mice
Phosphodiesterase Type 4 Inhibitor Rolipram Improves Survival of Spiral Ganglion Neurons?In Vitro
Koh EJ, Kim KJ, Song JH, Choi J, Lee HY, Kang DH, Heo HJ, Lee BY. Int J Mol Sci. 2017 Nov 13;18(11). pii: E2401. doi: 10.3390/ijms18112401. Spirulina maxima Extract Ameliorates Learning and Memory Impairments via Inhibiting GSK-3β Phosphorylation ...
Schulze, J., Kaiser, O., Paasche, G., Lamm, H., Pich, A., Hoffmann, A.,..., & Warnecke, A. (2017). Effect of hyperbaric oxygen on BDNF-release and neuroprotection: Investigations with human mesenchymal stem cells and genetically modified NIH3T3 fi...
Chen, G., Ma, Q., Goswami, D., Shang, J., & Miller, G.M. (2017). Modulation of nuclear REST by alternative splicing: a potential therapeutic target for Huntington's disease. Journal of Cellular and Molecular Medicine. Advance online publication. d...
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Customer Q&As
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2 Customer Q&As for Mouse BDNF ELISA Kit EZ-Set™ (DIY Antibody Pairs)
Question
What is the best for diluting the plasma using EZ0309? Do you have an specific plasma diluent for this kit?
Verified customer
Asked: 2023-01-13
Answer
Please use Reagent Diluent for diluting the plasma for the Mouse BDNF ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0309). Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered. Our EZA001 includes this buffer. https://www.bosterbio.com/media/pdf/EZA001_DS.pdf
Boster Scientific Support
Answered: 2023-01-13
Question
Hello Boster Support: We are currently working on Western blot analysis of BDNF using your product Mouse BDNF ELISA Kit EZ-Set antibody, but we have a question about the positive control, where BDNF was detected in Western blot of CHO supernatant, are these normal CHO cells or these have any modifications?
Verified Customer
Verified customer
Asked: 2014-10-08
Answer
The CHO cell were transfected with human pro-BDNF expression vector, the BDNF was briefly expressed and detected from cell culture supernatant.
Boster Scientific Support
Answered: 2014-10-08