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Product Info Summary
| SKU: | M02163 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-CUG BP1 (CELF1) Mouse Monoclonal Antibody [Clone ID: OTI5B8]
SKU/Catalog Number
M02163
Size
100 µl
Description
Boster Bio Anti-CUGBP1 mouse monoclonal antibody, clone OTI5B8 (formerly 5B8). Catalog# M02163. Tested in IF, IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CUG BP1 (CELF1) Mouse Monoclonal Antibody [Clone ID: OTI5B8] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02163)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI5B8
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human CUGBP1 (NP_006551) produced in HEK293T cell.
Reactive Species
M02163 is reactive to CELF1 in Human
Observed Molecular Weight
52 kDa
Calculated molecular weight
52.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02163 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC: 1:50
IF: 1:100
Validation Images & Assay Conditions
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Anti-CUGBP1 mouse monoclonal antibody (M02163) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CUGBP1.
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Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-CUGBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CUGBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CUGBP1.
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Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-CUGBP1 monoclonal antibody (1:200).
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Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-CUGBP1 monoclonal antibody (1:200).
Specific Publications For Anti-CUG BP1 (CELF1) Mouse Monoclonal Antibody [Clone ID: OTI5B8] (M02163)
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