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Product Info Summary
| SKU: | A03179 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IP, IHC, WB |
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Product info
Product Name
Anti-Cul7/Cullin 7 Antibody
SKU/Catalog Number
A03179
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Cul7/Cullin 7 Antibody (Catalog # A03179). Tested in IHC, IP, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Cul7/Cullin 7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03179)
Host
Rabbit
Contents
0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminus region near amino acids 1675-1698 of Human Cul7 coupled to KLH.
Reactive Species
A03179 is reactive to CUL7 in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
191.2 kDa
Background of CUL7
Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 7 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 7 seems to be involved proteosomal degradation of proteins involved in endothelial proliferation and/or differentiation. Cullin 7 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1, FBXW8 and GLMN isoform 1. Cullin 7 interacts with a complex of SKP1 and FBXW8, but not with SKP1 alone.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03179 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:2,000 - 1:10,000
IHC: User optimized
IP: User optimized
WB: 1:500 - 1:1,000
Anti-Cul7 has been tested by immunohistochemistry. This antibody reacts with human and mouse Cul7 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and also immunoprecipitates protein from cell lysates (using HeLa, NIH-3T3, and others). This antibody also co-immunoprecipitates associated proteins in overexpressed CUL7 systems. A 191.2 kDa band corresponding to human Cul7 is detected. Most cell lines expressing Cul7 can be used as a positive control. Researchers should determine optimal titers for other applications.
Validation Images & Assay Conditions
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Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below.
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Boster's Anti-CUL7 antibody was diluted 1:500 to detect CUL7 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Specific Publications For Anti-Cul7/Cullin 7 Antibody (A03179)
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4 Customer Q&As for Anti-Cul7/Cullin 7 Antibody
Question
We are currently using anti-Cul7/Cullin 7 antibody A03179 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
The anti-Cul7/Cullin 7 antibody (A03179) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow using anti-Cul7/Cullin 7 antibody A03179. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-16
Question
I am interested in to test anti-Cul7/Cullin 7 antibody A03179 on human bone marrow for research purposes, then I may be interested in using anti-Cul7/Cullin 7 antibody A03179 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Walker
Verified customer
Asked: 2016-11-02
Answer
The products we sell, including anti-Cul7/Cullin 7 antibody A03179, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-11-02
Question
Will A03179 anti-Cul7/Cullin 7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Taylor
Verified customer
Asked: 2014-06-13
Answer
As indicated on the product datasheet, A03179 anti-Cul7/Cullin 7 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-06-13
