Anti-MPG Antibody

MPG antibody

Boster Bio Anti-MPG Antibody catalog # PA1825. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1825
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-MPG Antibody

View all MPG Antibodies

SKU/Catalog Number

PA1825

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MPG Antibody catalog # PA1825. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MPG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1825)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MPG, different from the related mouse sequence by two amino acids, and from related rat sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1825 is reactive to MPG in Human, Mouse, Rat

Applications

PA1825 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

33 kDa

Calculated molecular weight

32.869kDa

Background of MPG

MPG (N-methylpurine-DNA glycosylase) also known as MDG, 3-METHYLADENINE DNA GLYCOSYLASE, 3MeAde DNA GLYCOSYLASE, AAG or APNG. The MPG gene is mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines. The MPG gene was expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line (HT29). The completely characterized human MPG gene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of Apng to the repair of several mutagenic lesions in vivo, Hang et al. (1997) biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the Apng gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine (meG) was found in liver sections of APNG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the APNG-null animals.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For MPG (Source: Uniprot.org, NCBI)

Gene Name

MPG

Full Name

DNA-3-methyladenine glycosylase

Weight

32.869kDa

Superfamily

DNA glycosylase MPG family

Alternative Names

3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on16p; 3-alkyladenine DNA glycosylase; 3-methyladenine DNA glycosidase; AAG; ADPG; anpg; APNG; CRA36.1 (3-methyl-adenine DNA glycosylase); CRA36.1; DNA-3-methyladenine glycosylase; EC 3.2.2.21; MDGalkyladenine DNA glycosylase; Mid1; N-methylpurine-DNA glycosylase, MPG; N-methylpurine-DNA glycosylasePIG16; PIG11; proliferation-inducing protein 11; proliferation-inducing protein 16 MPG AAG, ADPG, APNG, CRA36.1, MDG, Mid1, PIG11, PIG16, anpg N-methylpurine DNA glycosylase DNA-3-methyladenine glycosylase|3 end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on 16p|3-alkyladenine DNA glycosylase|3-methyladenine DNA glycosidase|CRA36.1 (3-methyl-adenine DNA glycosylase)|N-methylpurine-DNA glycosylase, MPG|proliferation-inducing protein 11|proliferation-inducing protein 16

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MPG, check out the MPG Infographic

MPG infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MPG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-MPG Antibody

Question

Do you have a BSA free version of anti-MPG antibody PA1825 available?

Verified Customer

Verified customer

Asked: 2020-01-10

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-MPG antibody PA1825 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-10

Question

Is this PA1825 anti-MPG antibody reactive to the isotypes of MPG?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

The immunogen of PA1825 anti-MPG antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human MPG(228-243aa NKSFDQRDLAQDEAVW), different from the related mouse sequence by two amino acids, and from related rat sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-11

Question

I am looking for to test anti-MPG antibody PA1825 on human pancreas for research purposes, then I may be interested in using anti-MPG antibody PA1825 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-08-09

Answer

The products we sell, including anti-MPG antibody PA1825, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-08-09

Question

We are currently using anti-MPG antibody PA1825 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2018-04-20

Answer

The anti-MPG antibody (PA1825) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-04-20

Order DetailsPrice
PA1825

100μg

$370
PA1825-10ug

10μg sample (liquid)

$99
PA1825-Biotin

100 μg Biotin conjugated

$570
PA1825-Cy3

100 μg Cy3 conjugated

$570
PA1825-Dylight488

100 μg Dylight488 conjugated

$570
PA1825-Dylight550

100 μg Dylight550 conjugated

$570
PA1825-Dylight594

100 μg Dylight594 conjugated

$570
PA1825-FITC

100 μg FITC conjugated

$570
PA1825-HRP

100 μg HRP conjugated

$570
PA1825-APC

100 μg APC conjugated

$670
PA1825-PE

100 μg PE conjugated

$670
PA1825-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PA1825
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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