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Product Info Summary
| SKU: | PB9859 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CXCL5 Antibody Picoband®
SKU/Catalog Number
PB9859
PB0901 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CXCL5 Antibody Picoband® catalog # PB9859. Tested in ELISA, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CXCL5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9859)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat CXCL5 recombinant protein (Position: A38-Q130). Rat CXCL5 shares 58.7% and 76.3% amino acid (aa) sequence identity with human and mouse CXCL5, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9859 is reactive to Cxcl5 in Rat
Observed Molecular Weight
14 kDa
Calculated molecular weight
14.3 kDa
Background of Cxcl5
C-X-C motif chemokine 5 or ENA-78 is a protein that in humans is encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family. It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed ineosinophils, and can be inhibited with the type II interferon IFN-γ. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on human chromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodelling. Also, CXCL5 has been described to regulate neutrophil homeostasis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9859 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Rat
Validation Images & Assay Conditions
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Western blot analysis of CXCL5 using anti-CXCL5 antibody (PB9859).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant rat CXCL5 protein 0.5ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL5 antigen affinity purified polyclonal antibody (Catalog # PB9859) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL5 at approximately 14 kDa. The expected band size for CXCL5 is at 14 kDa.
Specific Publications For Anti-CXCL5 Antibody Picoband® (PB9859)
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4 Customer Q&As for Anti-CXCL5 Antibody Picoband®
Question
Our team were content with the WB result of your anti-CXCL5 antibody. However we have observed positive staining in platelet secreted. using this antibody. Is that expected? Could you tell me where is CXCL5 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
According to literature, platelet does express CXCL5. Generally CXCL5 expresses in secreted. Regarding which tissues have CXCL5 expression, here are a few articles citing expression in various tissues:
Epithelium, Pubmed ID: 1744577
Kidney, Pubmed ID: 15489334
Peripheral blood monocyte, Pubmed ID: 10095777
Platelet, Pubmed ID: 12665801
Boster Scientific Support
Answered: 2020-01-28
Question
We have seen staining in rat platelet. Any tips? Is anti-CXCL5 antibody supposed to stain platelet positively?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
From what I have seen in literature platelet does express CXCL5. From what I have seen in Uniprot.org, CXCL5 is expressed in smooth muscle tissue, kidney, epithelium, peripheral blood monocyte, platelet, among other tissues. Regarding which tissues have CXCL5 expression, here are a few articles citing expression in various tissues:
Epithelium, Pubmed ID: 1744577
Kidney, Pubmed ID: 15489334
Peripheral blood monocyte, Pubmed ID: 10095777
Platelet, Pubmed ID: 12665801
Boster Scientific Support
Answered: 2019-10-22
Question
We ordered your anti-CXCL5 antibody for ELISA on platelet in a previous experiment. I am using rat, and We want to use the antibody for WB next. We want examining platelet as well as smooth muscle tissue in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-11-15
Answer
I looked at the website and datasheets of our anti-CXCL5 antibody and it appears that PB9859 has been tested on rat in both ELISA and WB. Thus PB9859 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-11-15
Question
We are currently using anti-CXCL5 antibody PB9859 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-14
Answer
The anti-CXCL5 antibody (PB9859) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-14
