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Product Info Summary
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Anti-CXCL5 Antibody Picoband™
Boster Bio Anti-CXCL5 Antibody Picoband™ catalog # PB9859. Tested in ELISA, WB applications. This antibody reacts with Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CXCL5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9859)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived rat CXCL5 recombinant protein (Position: A38-Q130). Rat CXCL5 shares 58.7% and 76.3% amino acid (aa) sequence identity with human and mouse CXCL5, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
PB9859 is reactive to Cxcl5 in Rat
PB9859 is guaranteed for ELISA, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
ELISA , 0.1-0.5μg/ml, Rat, -
Western blot, 0.1-0.5μg/ml, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CXCL5 using anti-CXCL5 antibody (PB9859).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant rat CXCL5 protein 0.5ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL5 antigen affinity purified polyclonal antibody (Catalog # PB9859) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL5 at approximately 14 kDa. The expected band size for CXCL5 is at 14 kDa.
Gene/Protein Information For Cxcl5 (Source: Uniprot.Org, NCBI)
C-X-C motif chemokine 5
intercrine alpha (chemokine CxC) family
LIX*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Cxcl5, check out the Cxcl5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cxcl5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for PB9859
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