Anti-Desmin Antibody Picoband™ (monoclonal, 2B5)

Boster Bio Anti-Desmin Antibody Picoband™ (monoclonal, 2B5) catalog # M01948-3. Tested in Flow Cytometry, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: M01948-3
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IHC-P, WB

Product Name

Anti-Desmin Antibody Picoband™ (monoclonal, 2B5)

See all Desmin products

SKU/Catalog Number

M01948-3

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Desmin Antibody Picoband™ (monoclonal, 2B5) catalog # M01948-3. Tested in Flow Cytometry, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Desmin Antibody Picoband™ (monoclonal, 2B5) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01948-3)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

2B5

Isotype

IgG2b

Immunogen

E.coli-derived human Desmin recombinant protein (Position: M1-T304). Human Desmin shares 97% amino acid (aa) sequence identity with both mouse and rat Desmin.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M01948-3 is reactive to DES in Human, Mouse, Rat

Applications

M01948-3 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For DES (Source: Uniprot.Org, NCBI)

Uniprot ID

P17661

Gene ID

1674

Gene Name

DES

Full Name

Desmin

Weight

53.536kDa

Superfamily

intermediate filament family

Alternative Names

CMD1I; CMD1IFLJ41013; CSM1; CSM2; DES; Desmin; FLJ12025; FLJ39719; FLJ41793; intermediate filament protein DES CDCD3, CSM1, CSM2, LGMD1D, LGMD1E, LGMD2R desmin desmin|cardiomyopathy, dilated 1F (autosomal dominant)|epididymis secretory sperm binding protein|intermediate filament protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on DES, check out the DES Infographic

DES infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for DES: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M01948-3 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Qian Q, Qian H, Zhang X, Zhu W, Yan Y, Ye S, Peng X, Li W, Xu Z, Sun L, Xu W. Stem Cells Dev. 2012 Jan;21(1):67-75. Doi: 10.1089/Scd.2010.0519. Epub 2011 Jun 1. 5-Azacytidine Induces Cardiac Differentiation Of Human Umbilical Cord-Derived Mesenchy...

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5 Customer Q&As for Anti-Desmin Antibody Picoband™ (monoclonal, 2B5)

Question

Our lab were content with the WB result of your anti-Desmin antibody (monoclonal, 2B5). However we have been able to see positive staining in muscle cytoplasm using this antibody. Is that expected? Could you tell me where is DES supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-09-10

Answer

Based on literature, muscle does express DES. Generally DES expresses in cytoplasm, myofibril, sarcomere, z line. Regarding which tissues have DES expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 8792816, 15489334
Skeletal muscle, Pubmed ID: 10545598

Boster Scientific Support

Answered: 2019-09-10

Question

you antibody using your anti-Desmin antibody (monoclonal, 2B5) for myopathy studies. Has this antibody been tested with western blotting on k562 whole cell lysates? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-05-14

Answer

Thanks for your inquiry. This M01948-3 anti-Desmin antibody (monoclonal, 2B5) is tested on rat heart tissue, skeletal muscle tissue, mouse heart tissue, human k562, k562 whole cell lysates, liver tissue. It is guaranteed to work for Flow Cytometry, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-05-14

Question

We have tried in the past anti-Desmin antibody (monoclonal, 2B5) for IHC-P on liver in the past. I am using mouse, and We are going to use the antibody for WB next. My lab would like examining liver as well as muscle in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2018-12-27

Answer

I viewed the website and datasheets of our anti-Desmin antibody (monoclonal, 2B5) and it appears that M01948-3 has been tested on mouse in both IHC-P and WB. Thus M01948-3 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-12-27

Question

We have observed staining in rat skeletal muscle. Are there any suggestions? Is anti-Desmin antibody (monoclonal, 2B5) supposed to stain skeletal muscle positively?

C. Mitchell

Verified customer

Asked: 2018-02-16

Answer

From literature skeletal muscle does express DES. From Uniprot.org, DES is expressed in esophagogastric junction muscularis propria, muscle, skeletal muscle, liver, among other tissues. Regarding which tissues have DES expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 8792816, 15489334
Skeletal muscle, Pubmed ID: 10545598

Boster Scientific Support

Answered: 2018-02-16

Question

We are currently using anti-Desmin antibody (monoclonal, 2B5) M01948-3 for mouse tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?

C. Zhao

Verified customer

Asked: 2017-12-07

Answer

The anti-Desmin antibody (monoclonal, 2B5) (M01948-3) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-12-07

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