Rat CXCL5/LIX/ENA-78 ELISA Kit PicoKine™

Rat CXCL5/LIX/ENA-78 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Cxcl5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

Product Info Summary

SKU: EK0823
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Rat CXCL5/LIX/ENA-78 ELISA Kit PicoKine™

See all Cxcl5 products

SKU/Catalog Number

EK0823

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat CXCL5/LIX/ENA-78 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Cxcl5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat CXCL5/LIX/ENA-78 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0823)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A38-Q130

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0823 is reactive to Cxcl5 in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0823 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0823-CAP Anti-Rat Cxcl5 Pre-coated 96-well strip microplate 1
EK0823-ST Rat Cxcl5 Standard 2 vials, 10 ng/tube
EK0823-DA Rat Cxcl5 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0080.0380.0930.1950.3810.7931.4562.357

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Cxcl5, Dilution ratio of 1:100, concentration in serum is around 20ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat CXCL5/LIX/ENA-78 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3817043939159411
Standard deviation1.527.1432.041.987.9530.82
CV (%)4%4.2%7.3%7.5%%5%7.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Cxcl5 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 138363736360.822.2%
Sample 21701641741671683.692.1%
Sample 343951350145647730.646.4%
*number of samples for each test n=16.

Gene/Protein Information For Cxcl5 (Source: Uniprot.Org, NCBI)

Uniprot ID

P97885

Gene ID

Gene Name

Cxcl5

Full Name

C-X-C motif chemokine 5

Weight

14.263kDa

Superfamily

intercrine alpha (chemokine CxC) family

Alternative Names

LIX

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cxcl5, check out the Cxcl5 Infographic

Cxcl5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cxcl5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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9 Customer Q&As for Rat CXCL5/LIX/ENA-78 ELISA Kit PicoKine™

Question

What is the gene ID of EK0823?

Verified customer

Asked: 2021-02-22

Answer

The gene ID of the Rat CXCL5/LIX/ENA-78 ELISA Kit PicoKine™ (EK0823) is 60665, Cxcl6 C-X-C motif chemokine ligand 6 [ Rattus norvegicus (Norway rat) ].

Boster Scientific Support

Answered: 2021-02-23

Question

Q: can I use citrate plasma as samples in Rat CXCL5 Picokine® ELISA Kit (Catalog # EK0823)?

Verified Customer

Verified customer

Asked: 2020-10-10

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of CXCL5 causing disruption of its protein structure. CXCL5 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CXCL5 ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-10-10

Question

Q: how can I thaw whole blood sample for CXCL5 ELISA after freezing?

C. Williams

Verified customer

Asked: 2020-09-01

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CXCL5 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-09-01

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

P. Evans

Verified customer

Asked: 2020-07-31

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-07-31

Question

I am interested in your EK0823 Rat CXCL5/LIX/ENA-78 PicoKine

Verified Customer

Verified customer

Asked: 2019-10-11

Answer

We haven't detected CXCL6 samples with EK0823. According to the sequence homology, EK0823 will most likely react with CXCL6. The standard of the kit is Cxcl5.

Boster Scientific Support

Answered: 2019-10-11

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement CXCL5 using the EK0823 Rat CXCL5 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-10-08

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2019-10-08

Question

Q: if the enzyme conjugated CXCL5 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CXCL5 antigen?

M. Smith

Verified customer

Asked: 2018-04-02

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-04-02

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2017-10-17

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2017-10-17

Question

Q: Can CXCL5 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

D. Moore

Verified customer

Asked: 2016-02-05

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2016-02-05

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