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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
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Rat CXCL5/LIX/ENA-78 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat CXCL5/LIX/ENA-78 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Cxcl5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat CXCL5/LIX/ENA-78 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0823)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: A38-Q130
15.6 pg/ml - 1,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0823 is reactive to Cxcl5 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
EK0823 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Cxcl5
C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.
|EK0823-CAP||Anti-Rat Cxcl5 Pre-coated 96-well strip microplate||1|
|EK0823-ST||Rat Cxcl5 Standard||2 vials, 10 ng/tube|
|EK0823-DA||Rat Cxcl5 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat CXCL5 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Cxcl5, Dilution ratio of 1:100, concentration in serum is around 20ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat CXCL5/LIX/ENA-78 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Cxcl5 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Cxcl5 (Source: Uniprot.Org, NCBI)
C-X-C motif chemokine 5
intercrine alpha (chemokine CxC) family
LIX*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Cxcl5, check out the Cxcl5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cxcl5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EK0823
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