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- Table of Contents
CXCR2 antibody (A00455-1) performs well in Flow Cytometry, IHC and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Flow Cytometry analysis of human PBMC cells using anti-CXCR2 antibody (A00455-1).
Overlay histogram showing human PBMC cells stained with A00455-1 (Blue line). The cells fixed with 4% paraformaldehyde and were blocked with 10% normal goat serum. And then incubated with rabbit anti-CXCR2 Antibody (A00455-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing human PBMC cells stained with A00455-1 (Blue line). The cells fixed with 4% paraformaldehyde and were blocked with 10% normal goat serum. And then incubated with rabbit anti-CXCR2 Antibody (A00455-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-CXCR2 Antibody
CXCR2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IHC |
Tested Applications
CXCR2 antibody was positively detected by IHC in:
human spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CXCR2 antibody was positively detected by FC in:
human PBMC cell
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-CXCR2 Antibody
View all CXCR2 Antibodies
View all CXCR2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR2, which shares 52.4% amino acid (aa) sequence identity with rat CXCR2.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Calculated Molecular Weight
40.8 kDa
Background of CXCR2
CXCR2 is a receptor for Interleukin 8, which is a powerful neutrophil chemotactic factor. It is a member of the GPCR family (subfamily, chemokine). Binding of IL8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL8 with a high affinity and to GRO/MGSA and NAP2 also with a high affinity. It has been reported to be expressed in a wide variety of tissues. ESTs have been isolated from human placenta and thymus libraries.
Cite This Product
Anti-CXCR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00455-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
76%
Moderate Homology
Rabbit
67%
Low homology
Sheep
67%
Low homology
Cow
62%
Low homology
Rat
57%
Low homology
Dog
57%
Low homology
Mouse
52%
Low homology
Zebrafish
43%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for CXCR2 antibody
Flow Cytometry (FC) protocol for CXCR2 antibody
Publications
Application:
Species:
Cui D,Zhao Y,Xu J.Activation of CXCL5-CXCR2 axis promotes proliferation and accelerates G1 to S phase transition of papillary thyroid carcinoma cells and activates JNK and p38 pathways.Cancer Biol Ther.2019;20(5):608-616.doi:10.1080/15384047.2018.1539289.Epub 2018 Nov 7.PMID:30404567;PMCID:PMC6606038.
Species: Human,Mouse
PMID: 30404567
Anqi Xia,Huan Huang,Wenjun You et al.The neuroprotection of hyperbaric oxygen therapy against traumatic brain injury via NF-κB/MAPKs-CXCL1 signaling pathway, 28 December 2020, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.
Species: Rat
PMID: -
Activation of CXCL5-CXCR2 axis promotes proliferation and accelerates G1 to S phase transition of papillary thyroid carcinoma cells and activates JNK and p38 pathways Dong Cui 1, Yongfu Zhao 1, Jingchao Xu 1 Cancer Biol Ther. 2019;20(5):608-616. doi:
PMID: 30404567
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Product Promise
We promise Anti-CXCR2 Antibody performs excellently in relevant applications for Flow Cytometry, IHC. If it doesn't perform as promised, we will refund the product. Learn more
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A00455-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026