SKU PA1237
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC, WB

Overview

Product Name Anti-CXCR4 Antibody
SKU/Catalog Number PA1237
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for C-X-C chemokine receptor type 4(CXCR4) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-CXCR4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1237)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR4(18-33aa SGDYDSMKEPCFREEN), different from the related rat and mouse sequences by two amino acids.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Images And Assay Conditions

Figure 2. IHC analysis of CXCR4 using anti-CXCR4 antibody (PA1237).
CXCR4 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CXCR4 Antibody (PA1237) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 1. Western blot analysis of CXCR4 using anti-CXCR4 antibody (PA1237).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR4 antigen affinity purified polyclonal antibody (Catalog # PA1237) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR4 at approximately 40-43KD. The expected band size for CXCR4 is at 40KD.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P61073
Gene Name CXCR4
Protein Name C-X-C chemokine receptor type 4
Tissue Specificity Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested. .
Alternative Names C-X-C chemokine receptor type 4;CXC-R4;CXCR-4;FB22;Fusin;HM89;LCR1;Leukocyte-derived seven transmembrane domain receptor;LESTR;Lipopolysaccharide-associated protein 3;LAP-3;LPS-associated protein 3;NPYRL;Stromal cell-derived factor 1 receptor;SDF-1 receptor;CD184;CXCR4;
Subcellular Localization Cell membrane; Multi-pass membrane protein. Cell junction. Early endosome. Late endosome. Lysosome. In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated. In the presence of antigen, distributes to the immunological synapse forming at the T-cell-APC contact area, where it localizes at the peripheral and distal supramolecular activation cluster (SMAC).
Molecular Weight 39746 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. .
Research Areas Human, Mouse, Rat

*You can search these to find other products in these research areas.
Background CXCR4 (Chemokine,CXC Motif, Receptor 4), also known as FUSIN or NPY3R, is a protein that in humans is encoded by the CXCR4 gene. It is the receptor for the CXC chemokine SDF1 that has essential functions on embryo organogenesis, immunological functions and T lymphocyte trafficking. CXCR4 is the only SDF1 receptor identified so far. This suggests that CXCR4 expression is critical for the biological effects of SDF1. CXCR4 is also a seven-transmembrane-spanning, G-protein-coupled receptor for the CXC chemokine PBSF/SDF-1. It functions as a co-receptor for T-cell-line tropic human immunodeficiency virus HIV-1. It was concluded that PBSF/SDF-1 and CXCR4 define a new signalling system for organ vascularization.

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Polyclonal antibody for CXCR 4/CXCR4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CXCR 4/CXCR4 information: Molecular Weight: 39746 MW; Subcellular Localization: Cell membrane; Multi-pass membrane protein. Cell junction. Early endosome. Late endosome. Lysosome. In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated. In the presence of antigen, distributes to the immunological synapse forming at the T-cell-APC contact area, where it localizes at the peripheral and distal supramolecular activation cluster (SMAC); Tissue Specificity: Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.
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In stock
Order Product
PA1237
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

Troubleshooting

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Publications

Macrophage migration inhibitory factor%u2013CXCR4 is the dominant chemotactic axis in human mesenchymal stem cell recruitment to tumors
CXCL12 induces migration of oligodendrocyte precursor cells through the CXCR4%u2011activated MEK/ERK and PI3K/AKT pathways
Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer
Blockade of CXCL12/CXCR4 signaling inhibits intrahepatic cholangiocarcinoma progression and metastasis via inactivation of canonical Wnt pathway
Tacrolimus promotes hepatocellular carcinoma and enhances CXCR4/SDF-1? expression?in vivo
Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer
Wang J, Cai J, Han F, Yang C, Tong Q, Cao T, Wu L, Wang Z. Int J Gynecol Cancer. 2011 Aug;21(6):981-7. Doi: 10.1097/Igc.0B013E31821D2543. Silencing Of Cxcr4 Blocks Progression Of Ovarian Cancer And Depresses Canonical Wnt Signaling Pathway.
Li Y, Chen M, Yuan J, Zhou X, He W, Zu X, Qi L. Urol Int. 2014;92(2):157-63. Doi: 10.1159/000351952. Epub 2013 Aug 27. Cxcr4 Expression In Bladder Transitional Cell Carcinoma And Its Relationship With Clinicopathological Features.
Tan Xy, Chang S, Liu W, Tang Hh. Gut Liver. 2014 Mar;8(2):196-204. Doi: 10.5009/Gnl.2014.8.2.196. Epub 2013 Nov 5. Silencing Of Cxcr4 Inhibits Tumor Cell Proliferation And Neural Invasion In Human Hilar Cholangiocarcinoma.
Li S, Deng L, Gong L, Bian H, Dai Y, Wang Y. Neurosci Res. 2010 Aug;67(4):293-9. Doi: 10.1016/J.Neures.2010.04.006. Epub 2010 Apr 20. Upregulation Of Cxcr4 Favoring Neural-Like Cells Migration Via Akt Activation.
Lin F, Zheng Se, Shen Z, Tang Ln, Chen P, Sun Yj, Zhao H, Yao Y. Med Oncol. 2011 Jun;28(2):649-53. Doi: 10.1007/S12032-010-9493-4. Epub 2010 Mar 27. Relationships Between Levels Of Cxcr4 And Vegf And Blood-Borne Metastasis And Survival In Patients...
Chen Hy, Wang Jm, Wang Hy, Zhang Yx, Liu W, Pan L, Wang Wh, Chen Sf, Jin Wg, Wang L. Biomed Pharmacother. 2012 Oct;66(7):549-53. Doi: 10.1016/J.Biopha.2012.04.007. Epub 2012 May 24. Effect Of Short Hairpin Rna-Induced Cxcr4 Silence On Ovarian Canc...
Wang D, Guo S, Han Sy, Xu N, Guo Jy, Sun Q. Int J Oncol. 2013 May;42(5):1725-33. Doi: 10.3892/Ijo.2013.1841. Epub 2013 Feb 27. Distinct Roles Of Different Fragments Of Pdcd4 In Regulating The Metastatic Behavior Of B16 Melanoma Cells.
Zhang W, Wang X, Yue K, Liu S, Liu X. Biomed Res Int. 2015;2015:195828. Doi: 10.1155/2015/195828. Epub 2015 Mar 19. Construction Of A Cxcl12-Kdel Fusion Gene To Inhibit Head And Neck Squamous Cell Carcinoma Metastasis By Intracellular Sequestratio...
Liu W, Wang Y, Wang H, Wang A. Cell Biochem Biophys. 2014 Dec;70(3):1893-900. Doi: 10.1007/S12013-014-0148-5. Anticancer Effects Of Chemokine Receptor 4(Cxcr4) Gene Silenced By Cxcr4-Sirna In Nude Mice Model Of Ovarian Cancer.

Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to cxcr4 antibody, cd184 antibody, cxcr-4 antibody, lap3 antibody
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