Rabbit IgG polyclonal antibody for C-X-C chemokine receptor type 4(CXCR4) detection. Tested with WB, IHC-P in Human;Mouse;Rat. Cited in 15 publication(s).
Anti-CXCR4 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-CXCR4 Antibody
See all CXCR4 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for CXCR 4/CXCR4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CXCR 4/CXCR4 information: Molecular Weight: 39746 MW; Subcellular Localization: Cell membrane; Multi-pass membrane protein. Cell junction. Early endosome. Late endosome. Lysosome. In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated. In the presence of antigen, distributes to the immunological synapse forming at the T-cell-APC contact area, where it localizes at the peripheral and distal supramolecular activation cluster (SMAC); Tissue Specificity: Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.|
|Cite This Product||Anti-CXCR4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1237)|
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|Anti-CXCR4 Antibody may replace the following items: sc 12764|sc 366006|sc 376270|sc 398601|sc 53534|sc 6190|sc 6191|sc 6279|sc 82958|sc 82959|sc 82960|sc 9046.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR4(18-33aa SGDYDSMKEPCFREEN), different from the related rat and mouse sequences by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For CXCR4 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||7852|
|Species Of This Entry||Human|
|Protein Name||C-X-C chemokine receptor type 4|
|Superfamily||G-protein coupled receptor 1 family|
|Alternative Names||CXCR4|CD184; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CXCR4; CXC-R4; CXCR-4; D2S201E; D2S201ESDF-1 receptor; FB22; Fusin; HM89; HM89NPYRL; HSY3RR; LAP3; LAP-3; LCR1; LESTR; LESTRCD184 antigen; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; NPY3R; NPY3RWHIM; NPYR; NPYRL; NPYY3R; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor;|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Glycosylation; Methylation; Ubiquitination; Acetylation; Demethylation; Dephosphorylation; Biotinylation; Reduction; Carboxylation; Oxidation; Myristoylation; Formylation; Amidation; Prenylation; Deacetylation; Amination; Phosphorothioation; Citrullination|
|Subcellular Localization||Cell membrane; Multi-pass membrane protein. Cell junction. Early endosome. Late endosome. Lysosome. In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated. In the presence of antigen, distributes to the immunological synapse forming at the T-cell-APC contact area, where it localizes at the peripheral and distal supramolecular activation cluster (SMAC).|
|Gene Location||2q22.1, on Chromasome 2, gene sequence: NC_000002.12 (136114349..136118149, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CXCR4 (Source: Uniprot.org, NCBI)
|Protein Function||Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation (PubMed:10452968, PubMed:28978524, PubMed:18799424, PubMed:24912431). Involved in the AKT signaling cascade (PubMed:24912431). Plays a role in regulation of cell migration, e.g. during wound healing (PubMed:28978524). Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels (PubMed:20228059). Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed:11276205). Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal- neuron survival (By similarity).|
|Tissue Specificity||Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.|
|Interacting Proteins||ACKR3; ADRA1A; ADRA1B; ADRA1D; AVPR1A; CCR5; CD164; CD74; CXCR3; CXCR5; DBN1; MYH9; TLR2|
|Related Diseases||Hiv Infections; Immunologic Deficiency Syndromes; Malignant Neoplasms; Neoplasms; Neoplasm Metastasis; Tissue Adhesions; Acquired Immunodeficiency Syndrome; Inflammation; Cell Invasion; Tumor Angiogenesis; Carcinoma; Malignant Neoplasm Of Breast; Leukemia; Mammary Neoplasms; Pathologic Neovascularization; Malignant Paraganglionic Neoplasm; Infective Disorder; Virus Diseases|
|Related Pathways||Chemotaxis; Cell Migration; Pathogenesis; Angiogenesis; Tropism; Cell Proliferation; Secretion; Localization; Regeneration; Immune Response; Viral Replication; Cell Adhesion; Membrane Fusion; Cell Death; Cell Growth; Cell Activation; Cell Cycle; Reverse Transcription; Drug Resistance; Glycosylation|
|Background||CXCR4 (Chemokine,CXC Motif, Receptor 4), also known as FUSIN or NPY3R, is a protein that in humans is encoded by the CXCR4 gene. It is the receptor for the CXC chemokine SDF1 that has essential functions on embryo organogenesis, immunological functions and T lymphocyte trafficking. CXCR4 is the only SDF1 receptor identified so far. This suggests that CXCR4 expression is critical for the biological effects of SDF1. CXCR4 is also a seven-transmembrane-spanning, G-protein-coupled receptor for the CXC chemokine PBSF/SDF-1. It functions as a co-receptor for T-cell-line tropic human immunodeficiency virus HIV-1. It was concluded that PBSF/SDF-1 and CXCR4 define a new signalling system for organ vascularization.|
|Scientific References||PMID: 8329116 by Herzog H., et al. Molecular cloning, characterization, and localization of the human homolog to the reported bovine NPY Y3 receptor: lack of NPY binding and activation.|
PMID: 8234909 by Jazin E.E., et al. A proposed bovine neuropeptide Y (NPY) receptor cDNA clone, or its human homologue, confers neither NPY binding sites nor NPY responsiveness on transfected cells.
Our Boster Quality Guarantee for Anti-CXCR4 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
CXCR4 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CXCR4 Antibody (PA1237) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR4 antigen affinity purified polyclonal antibody (Catalog # PA1237) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR4 at approximately 40-43KD. The expected band size for CXCR4 is at 40KD.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 15
|Liu W, Wang Y, Wang H, Wang A. Cell Biochem Biophys. 2014 Dec;70(3):1893-900. Doi: 10.1007/S12013-014-0148-5. Anticancer Effects Of Chemokine Receptor 4(Cxcr4) Gene Silenced By Cxcr4-Sirna In Nude Mice Model Of Ovarian Cancer.||PubMed ID 25149650|
|Zhang W, Wang X, Yue K, Liu S, Liu X. Biomed Res Int. 2015;2015:195828. Doi: 10.1155/2015/195828. Epub 2015 Mar 19. Construction Of A Cxcl12-Kdel Fusion Gene To Inhibit Head And Neck Squamous Cell Carcinoma Metastasis By Intracellular Sequestratio...||PubMed ID 25866764|
|Wang D, Guo S, Han Sy, Xu N, Guo Jy, Sun Q. Int J Oncol. 2013 May;42(5):1725-33. Doi: 10.3892/Ijo.2013.1841. Epub 2013 Feb 27. Distinct Roles Of Different Fragments Of Pdcd4 In Regulating The Metastatic Behavior Of B16 Melanoma Cells.||PubMed ID 23450345|
|Chen Hy, Wang Jm, Wang Hy, Zhang Yx, Liu W, Pan L, Wang Wh, Chen Sf, Jin Wg, Wang L. Biomed Pharmacother. 2012 Oct;66(7):549-53. Doi: 10.1016/J.Biopha.2012.04.007. Epub 2012 May 24. Effect Of Short Hairpin Rna-Induced Cxcr4 Silence On Ovarian Canc...||PubMed ID 22902648|
|Lin F, Zheng Se, Shen Z, Tang Ln, Chen P, Sun Yj, Zhao H, Yao Y. Med Oncol. 2011 Jun;28(2):649-53. Doi: 10.1007/S12032-010-9493-4. Epub 2010 Mar 27. Relationships Between Levels Of Cxcr4 And Vegf And Blood-Borne Metastasis And Survival In Patients...||PubMed ID 20349215|
|Li S, Deng L, Gong L, Bian H, Dai Y, Wang Y. Neurosci Res. 2010 Aug;67(4):293-9. Doi: 10.1016/J.Neures.2010.04.006. Epub 2010 Apr 20. Upregulation Of Cxcr4 Favoring Neural-Like Cells Migration Via Akt Activation.||PubMed ID 20412823|
|Tan Xy, Chang S, Liu W, Tang Hh. Gut Liver. 2014 Mar;8(2):196-204. Doi: 10.5009/Gnl.2014.8.2.196. Epub 2013 Nov 5. Silencing Of Cxcr4 Inhibits Tumor Cell Proliferation And Neural Invasion In Human Hilar Cholangiocarcinoma.||PubMed ID 24672662|
|Li Y, Chen M, Yuan J, Zhou X, He W, Zu X, Qi L. Urol Int. 2014;92(2):157-63. Doi: 10.1159/000351952. Epub 2013 Aug 27. Cxcr4 Expression In Bladder Transitional Cell Carcinoma And Its Relationship With Clinicopathological Features.||PubMed ID 23988662|
|Wang J, Cai J, Han F, Yang C, Tong Q, Cao T, Wu L, Wang Z. Int J Gynecol Cancer. 2011 Aug;21(6):981-7. Doi: 10.1097/Igc.0B013E31821D2543. Silencing Of Cxcr4 Blocks Progression Of Ovarian Cancer And Depresses Canonical Wnt Signaling Pathway.||PubMed ID 21738044|
|Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer||PubMed ID 2596092|
|Tacrolimus promotes hepatocellular carcinoma and enhances CXCR4/SDF-1? expression?in vivo||PubMed ID 24912495|
|Blockade of CXCL12/CXCR4 signaling inhibits intrahepatic cholangiocarcinoma progression and metastasis via inactivation of canonical Wnt pathway||PubMed ID 25471741|
|Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer||PubMed ID 18983683|
|CXCL12 induces migration of oligodendrocyte precursor cells through the CXCR4%u2011activated MEK/ERK and PI3K/AKT pathways||PubMed ID 30221695|
|Macrophage migration inhibitory factor%u2013CXCR4 is the dominant chemotactic axis in human mesenchymal stem cell recruitment to tumors||PubMed ID 25712213|
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