Human JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™

Human JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human F11R in cell culture supernatants and serum. Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1349
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants and serum.

Product Name

Human JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™

See all JAM-A products

SKU/Catalog Number

EK1349

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human F11R in cell culture supernatants and serum. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1349)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S28-V238

Sensitivity

<10 pg/ml

Assay Range

46.9 pg/ml - 3,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1349 is reactive to F11R in Human samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK1349 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1349-CAP Anti-Human F11R Pre-coated 96-well strip microplate 1
EK1349-ST Human F11R Standard 2 vials, 10 ng/tube
EK1349-DA Human F11R Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)046.993.8187.537575015003000
O.D.0.0110.1220.1870.3140.6150.9231.4351.858

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)813251280733101209
Standard deviation5.8316.967.845.3216.4393.09
CV (%)7.2%5.2%5.3%7.3%5.3%7.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of JAM-A in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 181888593864.385%
Sample 23253063193263197.962.4%
Sample 31280130114091335133148.993.6%
*number of samples for each test n=16.

Gene/Protein Information For F11R (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9Y624

Gene ID

50848

Gene Name

F11R

Full Name

Junctional adhesion molecule A

Weight

32.583kDa

Superfamily

immunoglobulin superfamily

Alternative Names

CD321 antigen; CD321; F11 receptor; F11R; JAM-1; JAM1CD321; JAMA; JAM-A; JCAMJAM; Junctional adhesion molecule 1Platelet F11 receptor; junctional adhesion molecule A; PAM-1; PAM-1KAT; Platelet adhesion molecule 1 F11R CD321, JAM, JAM1, JAMA, JCAM, KAT, PAM-1 F11 receptor junctional adhesion molecule A|junctional adhesion molecule 1|platelet F11 receptor|platelet adhesion molecule 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on F11R, check out the F11R Infographic

F11R infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for F11R: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Human JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™

Question

Q: how to analyze ELISA data? I have performed JAM-A level in serum.

P. Surve

Verified customer

Asked: 2020-11-05

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-11-05

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement JAM-A using the EK1349 Human JAM-A Picokine® ELISA Kit?

D. Liu

Verified customer

Asked: 2020-07-04

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2020-07-04

Question

Q: how can I thaw whole blood sample for JAM-A ELISA after freezing?

K. Dayal

Verified customer

Asked: 2020-05-25

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test JAM-A for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-05-25

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-03-30

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-03-30

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-01-23

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-01-23

Question

Q: What is the optimal O.D. value for JAM-A ELISA kit? I used your JAM-A ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain JAM-A even though the O.D. values are not very high?

S. Zhou

Verified customer

Asked: 2018-02-04

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this JAM-A ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of JAM-A that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-02-04

Question

Could you let me know if EK1349 will work with human brain tissue homogenates (homogenates are in a 1% NP-40 lysis buffer)?

Verified Customer

Verified customer

Asked: 2013-01-22

Answer

Our lab hasn't woked on human brain tissue samples using EK1349. Endogenous biotin in tissue homogenates might introduce false positive signal. We recommend the customer to run a pilot test as described below to confirm the affect of endogeous biotin first. Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, the customer can work on the tissue sample by using the kit.

Boster Scientific Support

Answered: 2013-01-22

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