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Product Info Summary
| SKU: | M02068 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-JAM1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02068
BM4474 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-JAM1 Rabbit Monoclonal Antibody catalog # M02068. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-JAM1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02068)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FOH-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human JAM1
Reactive Species
M02068 is reactive to F11R in Human, Mouse, Rat
Observed Molecular Weight
38 kDa
Calculated molecular weight
32.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02068 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
WB: human Hacat whole cell, human A431 whole cell, human RT4 whole cell
IHC: human blader cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of F11R using anti-F11R antibody (M02068).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hacat whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-F11R antigen affinity purified monoclonal antibody (M02068) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for F11R at approximately 38 kDa. The expected band size for F11R is at 33 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human blader cancer, using JAM1 Antibody.
Specific Publications For Anti-JAM1 Rabbit Monoclonal Antibody (M02068)
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7 Customer Q&As for Anti-JAM1 Rabbit Monoclonal Antibody
Question
Would M02068 anti-JAM1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Zhao
Verified customer
Asked: 2020-02-03
Answer
As indicated on the product datasheet, M02068 anti-JAM1 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-03
Question
Would anti-JAM1 Rabbit Monoclonal antibody M02068 work on zebrafish WB with ovary?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
Our lab technicians have not validated anti-JAM1 Rabbit Monoclonal antibody M02068 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-JAM1 Rabbit Monoclonal antibody M02068 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish ovary in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovary using anti-JAM1 Rabbit Monoclonal antibody M02068. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-29
Question
Is a blocking peptide available for product anti-JAM1 Rabbit Monoclonal antibody (M02068)?
Verified Customer
Verified customer
Asked: 2019-02-06
Answer
We do provide the blocking peptide for product anti-JAM1 Rabbit Monoclonal antibody (M02068). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-02-06
Question
Is there a BSA free version of anti-JAM1 Rabbit Monoclonal antibody M02068 available?
Verified Customer
Verified customer
Asked: 2018-04-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-JAM1 Rabbit Monoclonal antibody M02068 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-05
Question
I was wanting to use your anti-JAM1 Rabbit Monoclonal antibody for WB for mouse ovary on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse ovary identification?
Verified Customer
Verified customer
Asked: 2017-08-23
Answer
As indicated on the product datasheet, M02068 anti-JAM1 Rabbit Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-23
Question
I have attached the WB image, lot number and protocol we used for ovary using anti-JAM1 Rabbit Monoclonal antibody M02068. Please let me know if you require anything else.
C. Banerjee
Verified customer
Asked: 2015-12-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-12-15
