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- Table of Contents
CCNB1 antibody (A00745-1) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
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Flow Cytometry analysis of A431 cells using anti-CCNB1 antibody (A00745-1).
Overlay histogram showing A431 cells stained with A00745-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCNB1 Antibody (A00745-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A431 cells stained with A00745-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCNB1 Antibody (A00745-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Cyclin B1/CCNB1 Antibody Picoband®
CCNB1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
CCNB1 antibody was positively detected by WB in:
human Hela whole cell, human HEK293 whole cell, human Jurkat whole cell, human Raji whole cell, human K562 whole cell, human U2OS whole cell, human CACO-2 whole cell, rat spleen tissue, mouse thymus tissue, mouse NIH-3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CCNB1 antibody was positively detected by IHC in:
human rectal cancer tissue, human tonsil tissue, rat testis tissue, human laryngeal squamous cell carcinoma tissue, human seminoma testis tissue, human endometrial adenocarcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CCNB1 antibody was positively detected by ICC/IF in:
U20S cell
CCNB1 antibody was positively detected by FC in:
A431 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Rat
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Cyclin B1/CCNB1 Antibody Picoband®
View all CCNB1 Antibodies
View all CCNB1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cyclin B1/CCNB1 recombinant protein (Position: M1-L383).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated Molecular Weight
48.3 kDa
Background of CCNB1
G2/mitotic-specific cyclin-B1 is a protein that in humans is encoded by the CCNB1 gene. It is mapped to 5q13.2. The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle.
Cite This Product
Anti-Cyclin B1/CCNB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00745-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Dog
91%
High Homology
Sheep
91%
High Homology
Rabbit
89%
Moderate Homology
Cow
89%
Moderate Homology
Mouse
86%
Moderate Homology
Rat
85%
Moderate Homology
Zebrafish
62%
Low homology
Drosophila
38%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CCNB1 antibody
Immunohistochemistry (IHC) protocol for CCNB1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CCNB1 antibody
Flow Cytometry (FC) protocol for CCNB1 antibody
Publications
Application:
Species:
Transcriptome analysis reveals the anticancer effects of fenbendazole on ovarian cancer: an in vitro and in vivo study
Published: 2024-12-30
Journal: BMC CANCER
Application: WB
Impact Factor: 3.40
Species: Human
PMID: 39736624
Bufadienolides from Chansu Injection Synergistically Enhances the Antitumor Effect of Erlotinib by Inhibiting the KRAS Pathway in Pancreatic Cancer
Published: 2024-12-16
Journal: Pharmaceuticals
Application: WB
Impact Factor: 4.30
Species: Human
PMID: 39770538
Inhibitory Effect of Salvia miltiorrhiza Extract and Its Active Components on Cervical Intraepithelial Neoplastic Cells
Published: 2022-02-27
Journal: MOLECULES
Application: WB
Impact Factor: 4.93
Species: Human
PMID: 35268683
PKMYT1, exacerbating the progression of clear cell renal cell carcinoma, is implied as a biomarker for the diagnosis and prognosis
Published: 2021-12-27
Journal: Aging-US
Application: WB
Impact Factor: 5.68
Species: Human
PMID: 34959223
Wall shear stress promotes intimal hyperplasia through the paracrine H2O2-mediated NOX-AKT-SVV axis
Published: 2018-05-27
Journal: LIFE SCIENCES
Application: WB
Impact Factor: 3.23
Species: Rabbit,Human
PMID: 29847774
Silencing of lncRNA ZFAS1 inhibits malignancies by blocking Wnt/β-catenin signaling in gastric cancer cells
Published: 2018-03-04
Journal: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Application: WB
Impact Factor: 1.26
Species: Human
PMID: 29424266
Active targeting co-delivery system based on hollow mesoporous silica nanoparticles for antitumor therapy in ovarian cancer stem-like cells
Published: 2017-07-18
Journal: ONCOLOGY REPORTS
Application: WB
Impact Factor: 2.66
Species: Human
PMID: 28731164
Cucurbitacin B inhibits growth and induces apoptosis through the JAK2/STAT3 and MAPK pathways in SH‑SY5Y human neuroblastoma cells
Published: 2014-04-24
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 1.48
Species: Human
PMID: 24789581
Specific COX-2 inhibitor, meloxicam, suppresses proliferation and induces apoptosis in human HepG2 hepatocellular carcinoma cells
Published: 2006-10-27
Journal: JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
Application: WB
Impact Factor: 1.72
Species: Human
PMID: 17074019
Review
Have you used Anti-Cyclin B1/CCNB1 Antibody Picoband®?
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1 Review For Anti-Cyclin B1/CCNB1 Antibody Picoband®
WB results showed that CCNB1 (A00745-1) was upregulated in the rat colon model group and reduced after treatment, with clear and specific bands.
| SKU | A00745-1 |
|---|---|
| Application | Western Blot |
| Sample | rat colon tissue |
| Sample Processing Description | RIPA lysis buffer containing PMSF (100:1) was used for lysis for 10 min, followed by centrifugation at 12,000 rpm for 15 min. The supernatant was collected, mixed with 5× loading buffer, denatured at 100°C for 10 min, and then subjected to SDS-PAGE. |
| Other Reagents | 5% non-fat milk |
| Primary Antibody | Cyclin B1/CCNB1 Antibody Picoband® |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054) |
| Secondary Incubation | 1:5000, 1 h in RT |
| Detection | Substrate: ECL substrate, Image system:ChemiDoc MP |
| Results Summary | The figure shows WB results of CCNB1 and the internal control Actin in rat colon across different groups; CCNB1 expression was elevated in the model group and decreased after traditional Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific, indicating satisfactory results. |
Shiyu Zhang, Liaoning University of Traditional Chinese Medicine
Verified customer
Submitted 2026-03-04
Product Promise
We promise Anti-Cyclin B1/CCNB1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00745-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026