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CDK1 antibody (PB9533) performs well in Flow Cytometry, IF, IHC, IHC-F, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 15 Publications

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Flow Cytometry analysis of U937 cells using anti-CDK1 antibody (PB9533).
Overlay histogram showing U937 cells stained with PB9533 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDK1 Antibody (PB9533,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-CDK1 Antibody Picoband®

Name: Anti-CDK1 Antibody Picoband®
Brand: Boster Bio
SKU: PB9533
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

CDK1 antibody

15 Publications|

1 Review

|Submit a Review

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Tested Applications

CDK1 antibody was positively detected by WB in:

Rat Thymus Tissue, Rat Spleen Tissue, MCF-7 Whole Cell, HELA Whole Cell, JURKAT Whole Cell, NIH3T3 Whole Cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

CDK1 antibody was positively detected by IHC in:

mouse testis tissue, rat testis tissue, human mammary cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

CDK1 antibody was positively detected by ICC/IF in:

U20S cell

CDK1 antibody was positively detected by FC in:

U937 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Rat

Tested

Recommend

Mouse

Tested

Recommend

Human

Tested

Recommend

Dog

Predicted

Rabbit

Predicted

Bovine

Predicted

Sheep

Predicted

Monkey

Predicted

Product Information

Product Name

Anti-CDK1 Antibody Picoband®
View all CDK1 Antibodies

Form

Lyophilized

Uniprot

P06493

Gene ID

983

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CDK1 recombinant protein (Position: L66-M297). Human CDK1 shares 97.8% and 98.3% amino acid (aa) sequence identity with mouse and rat CDK1, respectively.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

34 kDa

Calculated Molecular Weight

34.1 kDa

Background of CDK1

CDC2, Cell Division Cycle 2, is also known as CDK1 (Cyclin-dependent Kinase 1). CDC2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor that induces entry into mitosis and is universal among eukaryotes. In HeLa cells CDC2 is the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell cycle regulation. CDC2 gene is located on chromosome 10.

Cite This Product

Anti-CDK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9533)

Protocols

Western Blot protocol for CDK1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for CDK1 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CDK1 antibody

Review Protocol

Flow Cytometry (FC) protocol for CDK1 antibody

Review Protocol

Publications

ERK promotes miR34a/ISOC1 signaling to enhance all trans retinoic acid-induced myeloid differentiation in acute promyelocytic leukemia

Published: 2026-06-20

Journal: JOURNAL OF BIOLOGICAL CHEMISTRY

Application: WB

Impact Factor: 4.10

Species: Human

PMID: 42323085

SKU	PB9533
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	Samples were lysed in RIPA buffer containing PMSF protease inhibitor (100:1) for 10 min, centrifuged at 12,000 rpm for 15 min, and the supernatant was mixed with 5× loading buffer, boiled at 100 °C for 10 min, and loaded onto SDS-PAGE.
Other Reagents	5% Non-fat milk
Primary Antibody	CDK1 Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 h in RT
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The image shows WB results of CDK1 and the loading control Actin in rat colon across different groups; CDK1 expression was elevated in the model group and most effectively reduced in the high-dose herbal treatment group, with clear and distinct target bands.

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Anti-CDK1 Antibody Picoband®

CDK1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

ERK promotes miR34a/ISOC1 signaling to enhance all trans retinoic acid-induced myeloid differentiation in acute promyelocytic leukemia

tRF-31-PS5P4PW3FJHP inhibits hypoxia-induced proliferation of pulmonary artery endothelial cells by regulating EDN1 splicing via binding to LSM4

Bufadienolides from Chansu Injection Synergistically Enhances the Antitumor Effect of Erlotinib by Inhibiting the KRAS Pathway in Pancreatic Cancer

Dieting alleviates hyperuricemia and organ injuries in uricase-deficient rats via down-regulating cell cycle pathway

Administration of A. muciniphila ameliorates pulmonary arterial hypertension by targeting miR-208a-3p/NOVA1 axis

Genetic Deletion of FXR1 Reduces Intimal Hyperplasia and Induces Senescence in Vascular Smooth Muscle Cells

ATG10 overexpression is related to the dismal prognosis and promotes the growth and migration of hepatocellular carcinoma cells via cyclin B1/CDK1 and CDK2

Compound K is a potential clinical anticancer agent in prostate cancer by arresting cell cycle

Inhibitory Effect of Salvia miltiorrhiza Extract and Its Active Components on Cervical Intraepithelial Neoplastic Cells

Extract of Ganoderma sinensis spores induces cell cycle arrest of hepatoma cell via endoplasmic reticulum stress

Coxsackievirus A6 Induces Cell Cycle Arrest in G0/G1 Phase for Viral Production

Wall shear stress promotes intimal hyperplasia through the paracrine H2O2-mediated NOX-AKT-SVV axis

A novel cell cycle blocker extracted from Stellera chamaejasme L. inhibits the proliferation of hepatocarcinoma cells

Cucurbitacin B inhibits growth and induces apoptosis through the JAK2/STAT3 and MAPK pathways in SH‑SY5Y human neuroblastoma cells

XJW20, a novel oxoindole derivative, induces G2/M arrest and apoptosis selectively in K562 leukemia cell line

Review

Have you used Anti-CDK1 Antibody Picoband®?

1 Review For Anti-CDK1 Antibody Picoband®

In WB using Anti-CDK1 (Phospho-T450) antibody (Cat# PB9533-50 µL), CDK1 expression in rat colon was increased in the model group and most effectively reduced in the high-dose herbal treatment group, with clear and well-defined target bands.

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