Anti-CDK1 Antibody Picoband™

CDC2/CDK1 antibody

Boster Bio Anti-CDK1 Antibody Picoband™ catalog # PB9533. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 17 publication(s).

Product Info Summary

SKU: PB9533
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Product Name

Anti-CDK1 Antibody Picoband™

View all CDC2/CDK1 Antibodies

SKU/Catalog Number

PB9533

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CDK1 Antibody Picoband™ catalog # PB9533. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CDK1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9533)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human CDK1 recombinant protein (Position: L66-M297). Human CDK1 shares 97.8% and 98.3% amino acid (aa) sequence identity with mouse and rat CDK1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9533 is reactive to CDK1 in Human, Mouse, Rat

Applications

PB9533 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Background of CDC2/CDK1

CDC2, Cell Division Cycle 2, is also known as CDK1 (Cyclin-dependent Kinase 1). CDC2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor that induces entry into mitosis and is universal among eukaryotes. In HeLa cells CDC2 is the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell cycle regulation. CDC2 gene is located on chromosome 10.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For CDK1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P06493

Gene ID

983

Gene Name

CDK1

Full Name

Cyclin-dependent kinase 1

Weight

34.095kDa

Superfamily

protein kinase superfamily

Alternative Names

CDC2; CDC28A; CDC2MGC111195; CDK1; cell cycle controller CDC2; Cell division control protein 2 homolog; Cell division protein kinase 1; cyclin-dependent kinase 1; DKFZp686L20222; EC 2.7.11.22; EC 2.7.11.23; G1 to S and G2 to M; p34 protein kinase; P34CDC2 CDK1 CDC2, CDC28A, P34CDC2 cyclin dependent kinase 1 cyclin-dependent kinase 1|cell cycle controller CDC2|cell division control protein 2 homolog|cell division cycle 2, G1 to S and G2 to M|cell division protein kinase 1|p34 protein kinase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CDK1, check out the CDK1 Infographic

CDK1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CDK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9533 has been cited in 17 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

A novel cell cycle blocker extracted from Stellera chamaejasme L. inhibits the proliferation of hepatocarcinoma cells

Extract of Ganoderma sinensis spores induces cell cycle arrest of hepatoma cell via endoplasmic reticulum stress

Blakemore D,Vilaplana-Lopera N,Almaghrabi R,Gonzalez E,Moya M,Ward C,Murphy G,Gambus A,Petermann E,Stewart GS,García P.MYBL2 and ATM suppress replication stress in pluripotent stem cells.EMBO Rep.2021 Mar 28:e51120.doi:10.15252/embr.202051120.Epub ahead of print.PMID:33779025.
Species: Mouse
PB9533 usage in article: APP:WB, SAMPLE:ESCS, DILUTION:NA

A novel cell cycle blocker extracted from Stellera chamaejasme L. inhibits the proliferation of hepatocarcinoma cells

Human enterovirus 68 interferes with the host cell cycle to facilitate viral production

Coxsackievirus A6 induces cell cycle arrest in G0/G1 phase for viral production

Prostate cancer cell proliferation is suppressed by microRNA-3160-5p via targeting of F-box and WD repeat domain containing 8

APC/CCDC20 and APC/C play pivotal roles in the process of embryonic development in Artemia sinica

Dietary NiCl2 causes G2/M cell cycle arrest in the broiler's kidney

Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats

Zhang Q, Miao S, Li C, Cui K, Ge Q, Chen Z. Am J Transl Res. 2018 Mar 15;10(3):731-743. eCollection 2018. S-phase kinase-associated protein 2 impairs the inhibitory effects of miR-1236-3p on bladder tumors

Yang C, Yang J, Sun M, Yan J, Meng X, Ma T. Iubmb Life. 2013 May;65(5):435-44. Doi: 10.1002/Iub.1141. Epub 2013 Feb 26. Alantolactone Inhibits Growth Of K562/Adriamycin Cells By Downregulating Bcr/Abl And P-Glycoprotein Expression.

Zeng Zj, Xiang Sg, Xue Ww, Li Hd, Ma N, Ren Zj, Xu Zj, Jiao Ch, Wang Cy, Hu Wx. Biomed Pharmacother. 2014 Sep;68(7):887-92. Doi: 10.1016/J.Biopha.2014.07.018. Epub 2014 Jul 28. The Cell Death And Dna Damages Caused By The Tet-On Regulating Hsv-Tk/...

Ye Z, Chen Z, Chen W, Xie J, Yang H, Lou Y, Yu Y. Chem Biol Interact. 2010 Jan 5;183(1):133-41. Doi: 10.1016/J.Cbi.2009.10.015. Xjw20, A Novel Oxoindole Derivative, Induces G2/M Arrest And Apoptosis Selectively In K562 Leukemia Cell Line.

Zheng Q, Liu Y, Liu W, Ma F, Zhou Y, Chen M, Chang J, Wang Y, Yang G, He G. Mol Med Rep. 2014 Jul;10(1):89-94. Doi: 10.3892/Mmr.2014.2175. Epub 2014 Apr 24. Cucurbitacin B Inhibits Growth And Induces Apoptosis Through The Jak2/Stat3 And Mapk Pathw...

Lin P, Fu J, Zhao B, Lin F, Zou H, Liu L, Zhu C, Wang H, Yu X. Mol Biol Rep. 2011 Mar;38(3):1741-7. Doi: 10.1007/S11033-010-0288-7. Epub 2010 Sep 29. Fbxw8 Is Involved In The Proliferation Of Human Choriocarcinoma Jeg-3 Cells.

Ren J, Huang Q, Xu Y, Yang M, Yang J, Hu K. Anticancer Drugs. 2015 Jul;26(6):599-611. Doi: 10.1097/Cad.0000000000000224. Isoflavone Lupiwighteone Induces Cytotoxic, Apoptotic, And Antiangiogenic Activities In Du-145 Prostate Cancer Cells.

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6 Customer Q&As for Anti-CDK1 Antibody Picoband™

Question

We are currently using anti-CDK1 antibody PB9533 for mouse tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, monkey, mouse, rat. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

The anti-CDK1 antibody (PB9533) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-26

Question

Our lab used your anti-CDK1 antibody for WB on cervix carcinoma erythroleukemia in a previous experiment. I am using human, and We want to use the antibody for ICC next. I am interested in examining cervix carcinoma erythroleukemia as well as lymphoblast in our next experiment. Could give a recommendation on which antibody would work the best for ICC?

Verified Customer

Verified customer

Asked: 2019-08-08

Answer

I have checked the website and datasheets of our anti-CDK1 antibody and I see that PB9533 has been tested on human in both WB and ICC. Thus PB9533 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-08-08

Question

Has PB9533 been tested with human IHC-Fr sections and what is the suggested protocol for the testing?

Verified customer

Asked: 2019-05-19

Answer

The Anti-CDK1 Antibody Picoband™ (PB9533) has been tested for human IHC-Fr sections - Suggested Dilution: Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human.

Boster Scientific Support

Answered: 2019-05-20

Question

Our lab want to know about using your anti-CDK1 antibody for dna replication studies. Has this antibody been tested with western blotting on nih3t3 whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-10-04

Answer

Thank you for your inquiry. This PB9533 anti-CDK1 antibody is tested on rat thymus tissue, tissue lysate, spleen tissue, hela whole cell lysate, jurkat whole cell lysate, nih3t3 whole cell lysate, mouse testis tissue, mammary cancer tissue, u937 cells. It is guaranteed to work for Flow Cytometry, IF, IHC-P, ICC, WB in human, monkey, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-10-04

Question

Our lab were happy with the WB result of your anti-CDK1 antibody. However we have been able to see positive staining in cervix carcinoma erythroleukemia nucleus. cytoplasm. mitochondrion. using this antibody. Is that expected? Could you tell me where is CDK1 supposed to be expressed?

D. Edwards

Verified customer

Asked: 2017-03-27

Answer

From what I have seen in literature, cervix carcinoma erythroleukemia does express CDK1. Generally CDK1 expresses in nucleus. cytoplasm. mitochondrion. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-03-27

Question

We have been able to see staining in monkey mammary cancer. Are there any suggestions? Is anti-CDK1 antibody supposed to stain mammary cancer positively?

H. Moore

Verified customer

Asked: 2016-04-13

Answer

From literature mammary cancer does express CDK1. From Uniprot.org, CDK1 is expressed in intestine, mammary cancer, skin, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2016-04-13

Size

Total: $315

SKU:PB9533

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