Anti-Survivin Birc5 Rabbit Monoclonal Antibody

Survivin antibody

Boster Bio Anti-Survivin Birc5 Rabbit Monoclonal Antibody catalog # M00379-2. Tested in WB, IHC, IP applications. This antibody reacts with Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: M00379-2
Size: 100 μl
Reactive Species: Mouse, Rat
Host: Rabbit
Application: IP, IHC, WB

Product Name

Anti-Survivin Birc5 Rabbit Monoclonal Antibody

View all Survivin Antibodies

SKU/Catalog Number

M00379-2

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Survivin Birc5 Rabbit Monoclonal Antibody catalog # M00379-2. Tested in WB, IHC, IP applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Survivin Birc5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00379-2)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DGD-2

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Survivin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00379-2 is reactive to BIRC5 in Mouse, Rat

Applications

M00379-2 is guaranteed for IP, IHC, WB Boster Guarantee

Observed Molecular Weight

125 kDa

Calculated molecular weight

16.389kDa

Background of Survivin

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
IP 1:30

Validation Images & Assay Conditions

Gene/Protein Information For BIRC5 (Source: Uniprot.org, NCBI)

Gene Name

BIRC5

Full Name

Baculoviral IAP repeat-containing protein 5

Weight

16.389kDa

Superfamily

IAP family

Alternative Names

API4; apoptosis inhibitor 4; Apoptosis inhibitor survivin; baculoviral IAP repeat-containing 5; Baculoviral IAP Repeat-Containing Protein 5; BIRC5; EPR-1; IAP4; survivin variant 3 alpha; Survivin BIRC5 API4, EPR-1 baculoviral IAP repeat containing 5 baculoviral IAP repeat-containing protein 5|apoptosis inhibitor 4|apoptosis inhibitor survivin|survivin variant 3 alpha

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BIRC5, check out the BIRC5 Infographic

BIRC5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BIRC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00379-2 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Cai N, Liu Nn, Zhao N, Wan C, Hu Yd, Zhou Y, Chen L. Int J Ophthalmol. 2012;5(3):293-6. Doi: 10.3980/J.Issn.2222-3959.2012.03.08. Epub 2012 Jun 18. Expressions Of Survivin And Vascular Endothelial Growth Factor In A Murine Model Of Proliferative R...

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15 Customer Q&As for Anti-Survivin Birc5 Rabbit Monoclonal Antibody

Question

Is a blocking peptide available for product anti-Survivin Rabbit Monoclonal antibody (M00379-2)?

Verified Customer

Verified customer

Asked: 2020-02-12

Answer

We do provide the blocking peptide for product anti-Survivin Rabbit Monoclonal antibody (M00379-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-02-12

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for myeloid leukemia cell using anti-Survivin Rabbit Monoclonal antibody M00379-2. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-01-30

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-30

Question

I have a question about product M00379-2, anti-Survivin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-20

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00379-2 anti-Survivin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-20

Question

We have observed staining in mouse mammary cancer. Are there any suggestions? Is anti-Survivin Rabbit Monoclonal antibody supposed to stain mammary cancer positively?

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

Based on literature mammary cancer does express BIRC5. Based on Uniprot.org, BIRC5 is expressed in vagina, myeloid leukemia cell, mammary cancer, neuroblastoma, lung, mammary gland muscle, cervix carcinoma, among other tissues. Regarding which tissues have BIRC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Lung, Mammary gland, and Muscle, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 16329164
Myeloid leukemia cell, Pubmed ID: 14702039, 14741722

Boster Scientific Support

Answered: 2019-12-16

Question

We want to test anti-Survivin Rabbit Monoclonal antibody M00379-2 on mouse myeloid leukemia cell for research purposes, then I may be interested in using anti-Survivin Rabbit Monoclonal antibody M00379-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-11-27

Answer

The products we sell, including anti-Survivin Rabbit Monoclonal antibody M00379-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-27

Question

My boss were happy with the WB result of your anti-Survivin Rabbit Monoclonal antibody. However we have been able to see positive staining in mammary cancer cytoplasm using this antibody. Is that expected? Could you tell me where is BIRC5 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-10

Answer

Based on literature, mammary cancer does express BIRC5. Generally BIRC5 expresses in cytoplasm. Regarding which tissues have BIRC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Lung, Mammary gland, and Muscle, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 16329164
Myeloid leukemia cell, Pubmed ID: 14702039, 14741722

Boster Scientific Support

Answered: 2019-10-10

Question

We are currently using anti-Survivin Rabbit Monoclonal antibody M00379-2 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-23

Answer

The anti-Survivin Rabbit Monoclonal antibody (M00379-2) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-23

Question

Is this M00379-2 anti-Survivin Rabbit Monoclonal antibody reactive to the isotypes of BIRC5?

Verified Customer

Verified customer

Asked: 2019-07-09

Answer

The immunogen of M00379-2 anti-Survivin Rabbit Monoclonal antibody is A synthesized peptide derived from human Survivin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-07-09

Question

Is there a BSA free version of anti-Survivin Rabbit Monoclonal antibody M00379-2 available?

Verified Customer

Verified customer

Asked: 2018-08-14

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Survivin Rabbit Monoclonal antibody M00379-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-08-14

Question

Does M00379-2 anti-Survivin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-08-06

Answer

As indicated on the product datasheet, M00379-2 anti-Survivin Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-08-06

Question

Here is the WB image, lot number and protocol we used for myeloid leukemia cell using anti-Survivin Rabbit Monoclonal antibody M00379-2. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-04-23

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-04-23

Question

I see that the anti-Survivin Rabbit Monoclonal antibody M00379-2 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-01-18

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-01-18

Question

We ordered your anti-Survivin Rabbit Monoclonal antibody for IP on mammary cancer last year. I am using rat, and I plan to use the antibody for WB next. We are interested in examining mammary cancer as well as neuroblastoma in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2017-12-29

Answer

I have checked the website and datasheets of our anti-Survivin Rabbit Monoclonal antibody and it seems that M00379-2 has been validated on rat in both IP and WB. Thus M00379-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-12-29

Question

Will anti-Survivin Rabbit Monoclonal antibody M00379-2 work for WB with myeloid leukemia cell?

P. Williams

Verified customer

Asked: 2015-07-17

Answer

According to the expression profile of myeloid leukemia cell, BIRC5 is highly expressed in myeloid leukemia cell. So, it is likely that anti-Survivin Rabbit Monoclonal antibody M00379-2 will work for WB with myeloid leukemia cell.

Boster Scientific Support

Answered: 2015-07-17

Question

I was wanting to use your anti-Survivin Rabbit Monoclonal antibody for WB for mouse myeloid leukemia cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse myeloid leukemia cell identification?

A. Evans

Verified customer

Asked: 2013-06-05

Answer

As indicated on the product datasheet, M00379-2 anti-Survivin Rabbit Monoclonal antibody has been validated for IP, IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse myeloid leukemia cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-06-05

Order DetailsPrice
M00379-2

100μL

$370
M00379-2-30ul

30μL sample

$99
M00379-2-carrier-free

Carrier Free

$370

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M00379-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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