Anti-Cyclin B1/CCNB1 Antibody Picoband™

Cyclin B1 antibody

Boster Bio Anti-Cyclin B1/CCNB1 Antibody Picoband™ catalog # PB9104. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. Cited in 10 publication(s).

Product Info Summary

SKU: PB9104
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, ICC, WB

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Product Name

Anti-Cyclin B1/CCNB1 Antibody Picoband™

View all Cyclin B1 Antibodies

SKU/Catalog Number

PB9104

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cyclin B1/CCNB1 Antibody Picoband™ catalog # PB9104. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cyclin B1/CCNB1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9104)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Cyclin B1 recombinant protein (Position: M1-V433). Human Cyclin B1 shares 86% and 85% amino acid (aa) sequences identity with mouse and rat Cyclin B1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9104 is reactive to CCNB1 in Human

Applications

PB9104 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Background of Cyclin B1

CCNB also known as Cyclin B1, is a protein that in humans is encoded by the CCNB1 gene, and it is mapped to 5q13.2. The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. CCNB contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis. Its activation is well-regulated, and positive feedback loops ensure that once the cyclin B1-Cdk1 complex is activated, it is not deactivated.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For CCNB1 (Source: Uniprot.org, NCBI)

Uniprot ID

P14635

Gene ID

891

Gene Name

CCNB1

Full Name

G2/mitotic-specific cyclin-B1

Weight

48.337kDa

Superfamily

cyclin family

Alternative Names

CCNB; CCNB1; Cyclin B1; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1 CCNB1 CCNB cyclin B1 G2/mitotic-specific cyclin-B1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CCNB1, check out the CCNB1 Infographic

CCNB1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CCNB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

PB9104 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Knockdown of protein tyrosine phosphatase receptor U inhibits growth and motility of gastric cancer cells

Growth inhibitory effect and Chk1-dependent signaling involved in G2/M arrest on human gastric cancer cells induced by diallyl disulfide

Suppression of C-myc Expression Associates with Anti-Proliferation of Aloe-Emodin on Gastric Cancer Cells

LRIG2 promotes the proliferation and cell cycle progression of glioblastoma cells in vitro and in vivo through enhancing PDGFR? signaling

Dipalmitoylphosphatidic acid inhibits tumor growth in triple-negative breast cancer

mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation

The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum

Guo X, Guo N, Zhao J, Cai Y. Oncol Rep. 2017 Sep;38(3):1442-1450. doi: 10.3892/or.2017.5829. Epub 2017 Jul 18. Active targeting co-delivery system based on hollow mesoporous silica nanoparticles for antitumor therapy in ovarian cancer stem-like cells

Ren Y, Wang Q, Shi L, Yue W, Zhang C, Lei F. Anim Reprod Sci. 2011 Feb;123(3-4):187-91. Doi: 10.1016/J.Anireprosci.2011.01.002. Epub 2011 Jan 14. Effects Of Maternal And Dietary Selenium (Se-Enriched Yeast) On The Expression Of P34(Cdc2) And Cycli...

Ye Z, Chen Z, Chen W, Xie J, Yang H, Lou Y, Yu Y. Chem Biol Interact. 2010 Jan 5;183(1):133-41. Doi: 10.1016/J.Cbi.2009.10.015. Xjw20, A Novel Oxoindole Derivative, Induces G2/M Arrest And Apoptosis Selectively In K562 Leukemia Cell Line.

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1 Customer Q&As for Anti-Cyclin B1/CCNB1 Antibody Picoband™

Question

I am interested in using your anti-Cyclin B1/CCNB1 antibody for mitotic cell cycle phase transition studies. Has this antibody been tested with western blotting on colo320 whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

We appreciate your inquiry. This PB9104 anti-Cyclin B1/CCNB1 antibody is tested on hela whole cell lysate, 293t whole cell lysate, colo320 whole cell lysate. It is guaranteed to work for Flow Cytometry, IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-13

Size

Conjugation

Total: $315

SKU:PB9104

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PB9104
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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