Anti-FLIP/CFLAR Antibody Picoband™

FLIP antibody

Boster Bio Anti-FLIP/CFLAR Antibody Picoband™ catalog # A01295-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A01295-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

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Product Name

Anti-FLIP/CFLAR Antibody Picoband™

View all FLIP Antibodies

SKU/Catalog Number

A01295-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FLIP/CFLAR Antibody Picoband™ catalog # A01295-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FLIP/CFLAR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01295-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human FLIP recombinant protein (Position: M1-D376).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01295-1 is reactive to CFLAR in Human, Mouse, Rat

Applications

A01295-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

43 kDa

Calculated molecular weight

55.344kDa

Background of FLIP

CASP8 and FADD-like apoptosis regulator, also called c-FLIP (FLICE-like inhibitory protein), is a protein that in humans is encoded by the CFLAR gene. The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For CFLAR (Source: Uniprot.org, NCBI)

Gene Name

CFLAR

Full Name

CASP8 and FADD-like apoptosis regulator

Weight

55.344kDa

Superfamily

peptidase C14A family

Alternative Names

CASHFADD-like antiapoptotic molecule 1; CASP8 and FADD-like apoptosis regulator; CASP8AP1; Caspase homolog; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; caspase-related inducer of apoptosis; CASPER; CasperFLAME1; Cellular FLICE-like inhibitory protein; CFLAR; c-FLIPR; c-FLIPS; CLARPMACH-related inducer of toxicity; FADD-like apoptotic molecule; FLAME; FLAME-1; FLIP; I-FLICE; I-FLICEMRITc-FLIPFLIPc-FLIPL; Inhibitor of FLICE; usurpin beta; Usurpin CFLAR CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS, cFLIP CASP8 and FADD like apoptosis regulator CASP8 and FADD-like apoptosis regulator|FADD-like antiapoptotic molecule 1|MACH-related inducer of toxicity|caspase homolog|caspase-eight-related protein|caspase-like apoptosis regulatory protein|caspase-related inducer of apoptosis|cellular FLICE-like inhibitory protein|inhibitor of FLICE|testis secretory sperm-binding protein Li 233m|usurpin beta

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CFLAR, check out the CFLAR Infographic

CFLAR infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CFLAR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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2 Customer Q&As for Anti-FLIP/CFLAR Antibody Picoband™

Question

Hi, I am interested in your A01295-1 FLIP antibody, it seems FLIP should express at around 55-60 Kda, however, your A01295-1 showed at 45 kda. So may I know if you have any reference showed 45 kda is correct and do you know what kind of isoform it is? (Reference CST #56343)

Verified Customer

Verified customer

Asked: 2020-03-11

Answer

Please see the following reference. FLIP Cleaved into the following 2 chains: subunit p43 and p12 https://www.uniprot.org/uniprot/O15519

Boster Scientific Support

Answered: 2020-03-11

Question

We are currently using anti-FLIP/CFLAR antibody A01295-1 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2017-06-27

Answer

The anti-FLIP/CFLAR antibody (A01295-1) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-06-27

Order DetailsPrice
A01295-1

100μg

$370
A01295-1-10ug

10μg sample (liquid)

$99
A01295-1-Biotin

100 μg Biotin conjugated

$570
A01295-1-Cy3

100 μg Cy3 conjugated

$570
A01295-1-Dylight488

100 μg Dylight488 conjugated

$570
A01295-1-Dylight550

100 μg Dylight550 conjugated

$570
A01295-1-Dylight594

100 μg Dylight594 conjugated

$570
A01295-1-FITC

100 μg FITC conjugated

$570
A01295-1-HRP

100 μg HRP conjugated

$570
A01295-1-APC

100 μg APC conjugated

$670
A01295-1-PE

100 μg PE conjugated

$670
A01295-1-carrier-free

Carrier Free

$370

More conjugates/formats

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A01295-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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