Anti-Cysteine protease ATG4D ATG4D Antibody

Product Name

Anti-Cysteine protease ATG4D ATG4D Antibody

View all ATG4D Antibodies

SKU/Catalog Number

A09793

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Cysteine protease ATG4D ATG4D Antibody (Catalog # A09793). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ATG4D antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Cysteine protease ATG4D ATG4D Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09793)

Host

Rabbit

Contents

ATG4D antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ATG4D antibody was raised against a 17 amino acid peptide near the amino terminus of human ATG4D. The immunogen is located within the first 50 amino acids of ATG4D.

Cross-reactivity

ATG4D antibody is human, mouse and rat reactive. At least two isoforms of ATG4D are known to exist; this antibody will detect only the larger isoform. ATG4D is predicted to not cross-react with other ATG4 proteins.

Reactive Species

A09793 is reactive to ATG4D in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

52.9 kDa

Background of ATG4D

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4D, also known as AUTL4, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3). ATG4 is required for ATG8 conjugation to phosphatidylethanolamine on autophagosomal membranes. In mammals, each ATG4 homolog shows a selective preference for the ATG8 homologs (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09793 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ATG4D antibody can be used for detection of ATG4D by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ATG4D

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