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Product Info Summary
| SKU: | M00598-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Cytochrome P450 1A2 (CYP1A2) Mouse Monoclonal Antibody [Clone ID: OTI6E2]
SKU/Catalog Number
M00598-1
Size
100 µl
Description
Boster Bio Anti-CYP1A2 (Cytochrome P450 1A2) mouse monoclonal antibody, clone OTI6E2 (formerly 6E2). Catalog# M00598-1. Tested in FC, IF, IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Cytochrome P450 1A2 (CYP1A2) Mouse Monoclonal Antibody [Clone ID: OTI6E2] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00598-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI6E2
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human CYP1A2 (NP_000752) produced in HEK293T cell.
Reactive Species
M00598-1 is reactive to CYP1A2 in Human
Calculated molecular weight
58.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00598-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
IHC 1:50
IF 1:100
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP1A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP1A2.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue N93ing anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded bladder tissue within the normal limits using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either CYP1A2 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CYP1A2 antibody (M00598-1)
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Flow cytometric Analysis of Jurkat cells
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Anti-CYP1A2 mouse monoclonal antibody (M00598-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP1A2.
Specific Publications For Anti-Cytochrome P450 1A2 (CYP1A2) Mouse Monoclonal Antibody [Clone ID: OTI6E2] (M00598-1)
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