This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
NFE2L2 antibody (M00078) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
Filter:
Immunofluorescent analysis of Hela cells, using Nrf2 Antibody.
Bundle Save
Anti-Nrf2 NFE2L2 Rabbit Monoclonal Antibody
NFE2L2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | IP, IF, IHC, ICC, WB |
Tested Applications
NFE2L2 antibody was positively detected by WB in:
293T cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
NFE2L2 antibody was positively detected by IHC in:
human kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
NFE2L2 antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:100-1:500
IP 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
IHC
IF
IP
Human
Tested
Recommend
Recommend
Mouse
Recommend
Product Information
Product Name
Anti-Nrf2 NFE2L2 Rabbit Monoclonal Antibody
View all NFE2L2 Antibodies
View all NFE2L2 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AGH-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Nrf2
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
90-110 kDa
Calculated Molecular Weight
67.8 kDa
Cite This Product
Anti-Nrf2 NFE2L2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00078)
Protocols
Western Blot protocol for NFE2L2 antibody
Immunohistochemistry (IHC) protocol for NFE2L2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for NFE2L2 antibody
Publications
Application:
Species:
Xin X,Yao D,Zhang K,Han S,Liu D,Wang H,Liu X,Li G,Huang J,Wang J.Protective effects of Rosavin on bleomycin-induced pulmonary fibrosis via suppressing fibrotic and inflammatory signaling pathways in mice.Biomed Pharmacother.2019 Jul;115:108870.doi:10.1016/j.biopha.2019.108870.Epub 2019 Apr 23.PMID:31026730.
Species: Mouse
PMID: 31026730
Chen M,Wang Z,Zhou W,Lu C,Ji T,Yang W,Jin Z,Tian Y,Lei W,Wu S,Fu Q,Wu Z,Wu X,Han M,Fang M,Yang Y.SIRT1/PGC-1α signaling activation by mangiferin attenuates cerebral hypoxia/reoxygenation injury in neuroblastoma cells.Eur J Pharmacol.2021 Jun 8:174236.doi:10.1016/j.ejphar.2021.174236.Epub ahead of print.PMID:34116043.
Species: Mouse
PMID: 34116043
Huanyue Cui,Xueying Liu,Jin Zhang,Ke Zhang,Dahong Yao,Shi Dong,Shushu Feng,Lu Yang, Yuyao Li,Hangyu Wang,Jian Huang,Jinhui Wang,"Rhodiola rosea L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways",Evidence-Based Complementary and Alternative Medicine,vol. 2021,Article ID 6103158,18 pages,2021.https://doi.org/10.1155/2021/6103158
Species: Human,Rat
PMID: -
Li D,Bai X,Jiang Y,Cheng Y.Butyrate alleviates PTZ-induced mitochondrial dysfunction, oxidative stress and neuron apoptosis in mice via Keap1/Nrf2/HO-1 pathway.Brain Res Bull.2020 Dec 28;168:25-35.doi:10.1016/j.brainresbull.2020.12.009.Epub ahead of print
Species: Mouse
PMID: 33359640
Li X,Zuo C,Sun D,Zhao T,Zhang Z.Arsenite Increases Linc-ROR in Human Bronchial Epithelial Cells that Can Be Inhibited by Antioxidant Factors.Biol Trace Elem Res.2020 Nov;198(1):131-141.doi:10.1007/s12011-020-02065-3.Epub 2020 Feb 6.PMID:32030632.
Species: Human
PMID: 32030632
Protective effects of berberine on renal injury in streptozotocin (STZ)-induced diabetic mice
PMID: 27529235
miR-155 mediates arsenic trioxide resistance by activating Nrf2 and suppressing apoptosis in lung cancer cells
PMID: 28939896
Protection of Nrf2 against arsenite-induced oxidative damage is regulated by the cyclic guanosine monophosphate-protein kinase G signaling pathway
PMID: 27774770
The protective role of vitamin D3 in a murine model of asthma via the suppression of TGF-?/Smad signaling and activation of the Nrf2/HO-1 pathway
PMID: 27484042
Review
Have you used Anti-Nrf2 NFE2L2 Rabbit Monoclonal Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
1 Review For Anti-Nrf2 NFE2L2 Rabbit Monoclonal Antibody
Using Anti‑NRF2 antibody (SKU: M00078), WB of normal, Aβ‑induced AD‑model and drug‑treated PC‑12 cells exhibits distinct target bands. Total NRF2 decreased upon Aβ stimulation and recovered after drug intervention.
| SKU | M00078 |
|---|---|
| Application | Western Blot |
| Sample | PC-12 (rat adrenal pheochromocytoma cells, highly differentiated) |
| Sample Processing Description | ① Normal PC12 cells (rat adrenal pheochromocytoma cells, highly differentiated)② PC12 cells are treated with β-amyloid (Aβ) to establish an in vitro Alzheimer's disease (AD) model.③ The AD in vitro model is treated with the therapeutic drug. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | Nrf2 NFE2L2 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated goat anti-rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in RT |
| Detection | Substrate: ECL substrate; Image system: ChemiDoc MP |
| Results Summary | ① Normal PC-12 cells (rat adrenal pheochromocytoma cells (highly differentiated)) ② PC12 cells were induced with β-amyloid protein (Aβ) to establish an in vitro model of Alzheimer's disease (AD) ③ The AD in vitro model was treated with therapeutic drugs. Experimental results showed that the total NRF2 protein expression level was downregulated after modeling, and the expression level rebounded after drug treatment. |
Yue Zhang, Chengdu University of Traditional Chinese Medicine
Verified customer
Submitted 2026-08-26
Product Promise
We promise Anti-Nrf2 NFE2L2 Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00078
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026