Anti-Cytochrome P450 2J2 CYP2J2 Antibody

Cytochrome p450 2J2 antibody

Boster Bio Anti-Cytochrome P450 2J2 CYP2J2 Antibody (Catalog # A03128). Tested in WB, IHC, IF applications. This antibody reacts with Human.

Product Info Summary

SKU: A03128
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: IF, IHC, WB

Product Name

Anti-Cytochrome P450 2J2 CYP2J2 Antibody

View all Cytochrome p450 2J2 Antibodies

SKU/Catalog Number

A03128

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Cytochrome P450 2J2 CYP2J2 Antibody (Catalog # A03128). Tested in WB, IHC, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cytochrome P450 2J2 CYP2J2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03128)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from inetrnal of human Cytochrome P450 2J2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03128 is reactive to CYP2J2 in Human

Applications

A03128 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

57.611kDa

Background of Cytochrome p450 2J2

This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools.

Wu S., J. Biol. Chem. 271:3460-3468(1996).
King L.M., Mol. Pharmacol. 61:840-852(2002).
The MGC Project Team, Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For CYP2J2 (Source: Uniprot.org, NCBI)

Gene Name

CYP2J2

Full Name

Cytochrome P450 2J2

Weight

57.611kDa

Superfamily

cytochrome P450 family

Alternative Names

Arachidonic acid epoxygenase; CPJ2; CYPIIJ2; cytochrome P450 2J2; cytochrome P450, family 2, subfamily J, polypeptide 2; cytochrome P450, subfamily IIJ (arachidonic acid epoxygenase) polypeptide 2; EC 1.14.14.1; flavoprotein-linked monooxygenase; microsomal monooxygenase CYP2J2 CPJ2, CYPIIJ2 cytochrome P450 family 2 subfamily J member 2 cytochrome P450 2J2|albendazole monooxygenase (hydroxylating)|albendazole monooxygenase (sulfoxide-forming)|arachidonic acid epoxygenase|cytochrome P450, family 2, subfamily J, polypeptide 2|cytochrome P450, subfamily IIJ (arachidonic acid epoxygenase) polypeptide 2|flavoprotein-linked monooxygenase|hydroperoxy icosatetraenoate isomerase|microsomal monooxygenase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CYP2J2, check out the CYP2J2 Infographic

CYP2J2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CYP2J2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-Cytochrome P450 2J2 CYP2J2 Antibody

Question

We are currently using anti-Cytochrome P450 2J2 antibody A03128 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

The anti-Cytochrome P450 2J2 antibody (A03128) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-29

Question

I was wanting to use your anti-Cytochrome P450 2J2 antibody for WB for human jejunal mucosa on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human jejunal mucosa identification?

L. Wu

Verified customer

Asked: 2018-10-08

Answer

As indicated on the product datasheet, A03128 anti-Cytochrome P450 2J2 antibody has been validated for IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human jejunal mucosa in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-10-08

Question

See below the WB image, lot number and protocol we used for jejunal mucosa using anti-Cytochrome P450 2J2 antibody A03128. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-04-25

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-04-25

Order DetailsPrice
A03128-100uL

100uL

$360

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