Product Info Summary
SKU: | A03128 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Cytochrome P450 2J2 CYP2J2 Antibody
View all Cytochrome p450 2J2 Antibodies
SKU/Catalog Number
A03128
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Cytochrome P450 2J2 CYP2J2 Antibody (Catalog # A03128). Tested in WB, IHC, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome P450 2J2 CYP2J2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03128)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from inetrnal of human Cytochrome P450 2J2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03128 is reactive to CYP2J2 in Human
Reconstitution
Calculated molecular weight
57611 MW
Background of Cytochrome p450 2J2
This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools.
Wu S., J. Biol. Chem. 271:3460-3468(1996).
King L.M., Mol. Pharmacol. 61:840-852(2002).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03128 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human heart tissue using Cytochrome P450 2J2 antibody A03128.
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Western blot analysis of extracts from A549 cells, RAW264.7 cells and NIH-3T3 cells, using Cytochrome P450 2J2 antibody A03128.
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Immunofluorescence analysis of HepG2 cells, using Cytochrome P450 2J2 antibody A03128.
Protein Target Info & Infographic
Gene/Protein Information For CYP2J2 (Source: Uniprot.org, NCBI)
Gene Name
CYP2J2
Full Name
Cytochrome P450 2J2
Weight
57611 MW
Superfamily
cytochrome P450 family
Alternative Names
Cytochrome P450 2J2;1.14.14.1;Arachidonic acid epoxygenase;CYPIIJ2;CYP2J2; CYP2J2 CPJ2, CYPIIJ2 cytochrome P450 family 2 subfamily J member 2 cytochrome P450 2J2|albendazole monooxygenase (hydroxylating)|albendazole monooxygenase (sulfoxide-forming)|arachidonic acid epoxygenase|cytochrome P450, family 2, subfamily J, polypeptide 2|cytochrome P450, subfamily IIJ (arachidonic acid epoxygenase) polypeptide 2|flavoprotein-linked monooxygenase|hydroperoxy icosatetraenoate isomerase|microsomal monooxygenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP2J2, check out the CYP2J2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP2J2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytochrome P450 2J2 CYP2J2 Antibody (A03128)
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No publications found for A03128
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Customer Q&As
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3 Customer Q&As for Anti-Cytochrome P450 2J2 CYP2J2 Antibody
Question
We are currently using anti-Cytochrome P450 2J2 antibody A03128 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The anti-Cytochrome P450 2J2 antibody (A03128) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-29
Question
I was wanting to use your anti-Cytochrome P450 2J2 antibody for WB for human jejunal mucosa on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human jejunal mucosa identification?
L. Wu
Verified customer
Asked: 2018-10-08
Answer
As indicated on the product datasheet, A03128 anti-Cytochrome P450 2J2 antibody has been validated for IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human jejunal mucosa in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-08
Question
See below the WB image, lot number and protocol we used for jejunal mucosa using anti-Cytochrome P450 2J2 antibody A03128. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-04-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-25