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CYP3A4 antibody (A00339-2) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of HL-60 cells using anti-Cytochrome P450 3A4/CYP3A4 antibody (A00339-2).
Overlay histogram showing HL-60 cells stained with A00339-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytochrome P450 3A4/CYP3A4 Antibody (A00339-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HL-60 cells stained with A00339-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytochrome P450 3A4/CYP3A4 Antibody (A00339-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Cytochrome P450 3A4/CYP3A4 Antibody Picoband®
CYP3A4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
CYP3A4 antibody was positively detected by WB in:
human SH-SY5Y whole cell, human HL-60 whole cell, human K562 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CYP3A4 antibody was positively detected by FC in:
HL-60 cell, U87 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-Cytochrome P450 3A4/CYP3A4 Antibody Picoband®
View all CYP3A4 Antibodies
View all CYP3A4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CYP3A4 recombinant protein (Position: L44-H267).
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
57 kDa
Calculated Molecular Weight
57.3 kDa
Background of CYP3A4
Cytochrome P450 3A4 (abbreviated CYP3A4), is an important enzyme in the body, mainly found in the liver and in the intestine. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified.
Cite This Product
Anti-Cytochrome P450 3A4/CYP3A4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00339-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
92%
High Homology
Dog
79%
Moderate Homology
Sheep
76%
Moderate Homology
Cow
75%
Moderate Homology
Rabbit
72%
Moderate Homology
Rat
72%
Moderate Homology
Mouse
70%
Moderate Homology
Zebrafish
53%
Low homology
Drosophila
30%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CYP3A4 antibody
Flow Cytometry (FC) protocol for CYP3A4 antibody
Review
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1 Review For Anti-Cytochrome P450 3A4/CYP3A4 Antibody Picoband®
The target band of this antibody is clear and at the correct position, and it performs better than antibodies from other brands.
| SKU | A00339-2 |
|---|---|
| Application | Western Blot |
| Sample | Human keratinocytes isolated from skin |
| Sample Processing Description | Keratinocytes isolated from normal skin tissue. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | Cytochrome P450 3A4/CYP3A4 Antibody |
| Primary Incubation | 1:5000, overnight at 4 ℃ |
| Secondary Antibody | HRP Goat Anti-Rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | CYP3A4 is a major enzyme responsible for drug metabolism in the body. More than 50% of commonly used drugs in humans are metabolized and cleared through CYP3A4. It catalyzes various chemical reactions, such as oxidation and reduction, converting drugs into more water-soluble forms that are easier to excrete. This experiment aimed to verify whether human skin keratinocytes synthesize CYP3A4 protein and to estimate its approximate level. The results show that skin keratinocytes do synthesize CYP3A4 protein, and its expression level is considerable. |
Xianyong Zhou, Hangzhou Jiexiao Testing Technology Co., Ltd.
Verified customer
Submitted 2025-12-08
Product Promise
We promise Anti-Cytochrome P450 3A4/CYP3A4 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00339-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026