Anti-Cytokeratin 17 (CK-17) KRT19 Rabbit Monoclonal Antibody, Clone#RM351

KRT17 antibody

Boster Bio Anti-Cytokeratin 17 (CK-17) KRT19 Rabbit Monoclonal Antibody, Clone#RM351 (Catalog # M02101-3). Tested in IHC, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: M02101-3
Size: 100uL
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-Cytokeratin 17 (CK-17) KRT19 Rabbit Monoclonal Antibody, Clone#RM351

View all KRT17 Antibodies

SKU/Catalog Number

M02101-3

Size

100uL

Form

Liquid

Description

Boster Bio Anti-Cytokeratin 17 (CK-17) KRT19 Rabbit Monoclonal Antibody, Clone#RM351 (Catalog # M02101-3). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cytokeratin 17 (CK-17) KRT19 Rabbit Monoclonal Antibody, Clone#RM351 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02101-3)

Host

Rabbit

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Clonality

Monoclonal

Clone Number

RM351

Isotype

Rabbit IgG

Immunogen

A peptide corresponding to residues in human Cytokeratin 17 (CK-17)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody reacts to human Cytokeratin 17 (CK-17)

Reactive Species

M02101-3 is reactive to KRT17 in Human

Applications

M02101-3 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

48.106kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:20000 dilution.

Validation Images & Assay Conditions

Gene/Protein Information For KRT17 (Source: Uniprot.org, NCBI)

Gene Name

KRT17

Full Name

Keratin, type I cytoskeletal 17

Weight

48.106kDa

Superfamily

intermediate filament family

Alternative Names

39.1; CK-17; cytokeratin-17; K17; keratin 17; keratin, type I cytoskeletal 17; keratin-17; PC; PC2; PCHC1 KRT17 39.1, CK-17, K17, PC, PC2, PCHC1 keratin 17 keratin, type I cytoskeletal 17|cytokeratin-17|keratin 17, type I

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on KRT17, check out the KRT17 Infographic

KRT17 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KRT17: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M02101-3 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Lu J, Gu Yp, Xu X, Liu Ml, Xie P, Song Hp. World J Gastroenterol. 2005 Jun 14;11(22):3426-30. Adult Islets Cultured In Collagen Gel Transdifferentiate Into Duct-Like Cells.

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15 Customer Q&As for Anti-Cytokeratin 17 (CK-17) KRT19 Rabbit Monoclonal Antibody, Clone#RM351

Question

Would M02101-3 anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-29

Answer

It shows on the product datasheet, M02101-3 anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-29

Question

My lab would like to test anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 M02101-3 on human lower esophagus mucosa for research purposes, then I may be interested in using anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 M02101-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-04-21

Answer

The products we sell, including anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 M02101-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-04-21

Question

I was wanting to use your anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 for WB for human lower esophagus mucosa on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lower esophagus mucosa identification?

Verified Customer

Verified customer

Asked: 2020-04-13

Answer

As indicated on the product datasheet, M02101-3 anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lower esophagus mucosa in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-13

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lower esophagus mucosa using anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 M02101-3. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-02-18

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-18

Question

Will anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 M02101-3 work for WB with lower esophagus mucosa?

Verified Customer

Verified customer

Asked: 2020-01-29

Answer

According to the expression profile of lower esophagus mucosa, KRT19 is highly expressed in lower esophagus mucosa. So, it is likely that anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 M02101-3 will work for WB with lower esophagus mucosa.

Boster Scientific Support

Answered: 2020-01-29

Question

My lab would like using your anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 for viral process studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-10-30

Answer

Thanks for your inquiry. This M02101-3 anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 is tested on hela cell lysate, human tonsil tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-10-30

Question

Is this M02101-3 anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 reactive to the isotypes of KRT19?

B. Wu

Verified customer

Asked: 2019-09-19

Answer

The immunogen of M02101-3 anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 is A peptide corresponding to residues in human Cytokeratin 17 (CK-17). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-09-19

Question

We are currently using anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 M02101-3 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-04

Answer

The anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 (M02101-3) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-04

Question

I see that the anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 M02101-3 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-12

Question

We have tried in the past anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

I took a look at the website and datasheets of our anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone

Boster Scientific Support

Answered: 2019-08-12

Question

Here is the WB image, lot number and protocol we used for lower esophagus mucosa using anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 M02101-3. Please let me know if you require anything else.

N. Anderson

Verified customer

Asked: 2019-07-09

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-09

Question

Is a blocking peptide available for product anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 (M02101-3)?

D. Zhao

Verified customer

Asked: 2019-06-26

Answer

We do provide the blocking peptide for product anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 (M02101-3). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-26

Question

We have observed staining in human peripheral blood leukocyte. What should we do? Is anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 supposed to stain peripheral blood leukocyte positively?

Verified Customer

Verified customer

Asked: 2019-05-14

Answer

From literature peripheral blood leukocyte does express KRT19. From Uniprot.org, KRT19 is expressed in lower esophagus mucosa, placenta, mammary gland, pancreas placenta, peripheral blood leukocyte, keratinocyte, lymph node, cervix carcinoma erythroleukemia, colon carcinoma, among other tissues. Regarding which tissues have KRT19 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Keratinocyte, Pubmed ID: 1286667
Lymph node, Pubmed ID: 10212274
Mammary gland, Pancreas, and Placenta, Pubmed ID: 15489334
Peripheral blood leukocyte, Pubmed ID: 11682035
Placenta, Pubmed ID: 2447559, 2469734, 14702039

Boster Scientific Support

Answered: 2019-05-14

Question

Can you help my question with product M02101-3, anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-10-10

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02101-3 anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-10-10

Question

Is there a BSA free version of anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 M02101-3 available?

J. Collins

Verified customer

Asked: 2013-12-13

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cytokeratin 17 (CK-17) Rabbit Monoclonal antibody, Clone#RM351 M02101-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-12-13

$431
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Lead time for this item is typically 1-2 weeks

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M02101-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$431.00

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