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Product Info Summary
| SKU: | PB9407 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Unrip/STRAP Antibody Picoband®
SKU/Catalog Number
PB9407
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Unrip/STRAP Antibody Picoband® catalog # PB9407. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Unrip/STRAP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9407)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Unrip, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9407 is reactive to STRAP in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
38.4 kDa
Background of STRAP
Unrip is also known as STRAP (Serine-threonine kinase receptor-associated protein). It is anenzyme that in humans is encoded by the STRAP gene. It is mapped to 12p12.3. UNRIP is integrated into a complex with UNR or with the SMN complex in vivo in a mutually exclusive manner. It is concluded that UNRIP is the first component of the uridine-rich snRNP assembly machinery that associates with the SMN complex in a compartment-specific way, and it may play a crucial role in the intracellular distribution of the SMN complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9407 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human HEK293 whole cell, human Jurkat whole cell, human PC-3 whole cell, human THP-1 whole cell, rat brain tissue, mouse HEPA1-6 whole cell, mouse RAW2467 whole cell,
IHC: human mammary cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Unrip using anti-Unrip antibody (PB9407).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates
Lane 2: human Jurkat whole cell lysates
Lane 3: human PC-3 whole cell lysates
Lane 4: human THP-1 whole cell lysates
Lane 5: rat brain tissue lysates
Lane 6: mouse HEPA1-6 whole cell lysates
Lane 7: mouse RAW246.7 whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Unrip antigen affinity purified polyclonal antibody (Catalog # PB9407) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Unrip at approximately 39KD. The expected band size for Unrip is at 39KD.
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IHC analysis of Unrip using anti-Unrip antibody (PB9407).
Unrip was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Unrip Antibody (PB9407) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Unrip/STRAP Antibody Picoband® (PB9407)
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Customer Q&As
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4 Customer Q&As for Anti-Unrip/STRAP Antibody Picoband®
Question
I see that the anti-Unrip/STRAP antibody PB9407 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-30
Question
Is this PB9407 anti-Unrip/STRAP antibody reactive to the isotypes of STRAP?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
The immunogen of PB9407 anti-Unrip/STRAP antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Unrip(78-104aa AADFTAKVWDAVSGDELMTLAHKHIVK), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-18
Question
Would anti-Unrip/STRAP antibody PB9407 work for IHC with brain cajal-retzius cell?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
According to the expression profile of brain cajal-retzius cell, STRAP is highly expressed in brain cajal-retzius cell. So, it is likely that anti-Unrip/STRAP antibody PB9407 will work for IHC with brain cajal-retzius cell.
Boster Scientific Support
Answered: 2019-07-11
Question
We are currently using anti-Unrip/STRAP antibody PB9407 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
D. Jones
Verified customer
Asked: 2014-10-20
Answer
The anti-Unrip/STRAP antibody (PB9407) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-20
