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Product Info Summary
| SKU: | A07219-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-D54 TPD52L2 Antibody
SKU/Catalog Number
A07219-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-D54 TPD52L2 Antibody catalog # A07219-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-D54 TPD52L2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07219-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human TPD54. AA range:141-190
Reactive Species
A07219-1 is reactive to TPD52L2 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
22.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07219-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:5000
Validation Images & Assay Conditions
There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.
Specific Publications For Anti-D54 TPD52L2 Antibody (A07219-1)
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Customer Q&As
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6 Customer Q&As for Anti-D54 TPD52L2 Antibody
Question
I have a question about product A07219-1, anti-D54 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07219-1 anti-D54 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-28
Question
I see that the anti-D54 antibody A07219-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-22
Question
Will anti-D54 antibody A07219-1 work on primate WB with placenta retinoblastoma?
J. Kulkarni
Verified customer
Asked: 2019-05-02
Answer
Our lab technicians have not tested anti-D54 antibody A07219-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-D54 antibody A07219-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate placenta retinoblastoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-02
Question
We want to test anti-D54 antibody A07219-1 on rat cervix carcinoma for research purposes, then I may be interested in using anti-D54 antibody A07219-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Krishna
Verified customer
Asked: 2018-08-22
Answer
The products we sell, including anti-D54 antibody A07219-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-08-22
Question
Will A07219-1 anti-D54 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-07-18
Answer
You can see on the product datasheet, A07219-1 anti-D54 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-18
Question
We are currently using anti-D54 antibody A07219-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
P. Roberts
Verified customer
Asked: 2017-04-25
Answer
The anti-D54 antibody (A07219-1) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-04-25
